| pubmed-article:12934081 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12934081 | lifeskim:mentions | umls-concept:C0043393 | lld:lifeskim |
| pubmed-article:12934081 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:12934081 | lifeskim:mentions | umls-concept:C0026237 | lld:lifeskim |
| pubmed-article:12934081 | lifeskim:mentions | umls-concept:C1704675 | lld:lifeskim |
| pubmed-article:12934081 | lifeskim:mentions | umls-concept:C1261322 | lld:lifeskim |
| pubmed-article:12934081 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:12934081 | pubmed:dateCreated | 2003-8-22 | lld:pubmed |
| pubmed-article:12934081 | pubmed:abstractText | Because of structural homology with the transmembrane domain of Bcl-2, the proapoptotic protein Bax has been proposed to be anchored to the outer membrane of mitochondria through its carboxy-terminal end (CT). We took advantage of the absence of Bcl-2 family members in yeast to further investigate the role of Bax CT in its mitochondrial association and function. The complete deletion or the addition of a C-terminal c-myc tag as well as the replacement of CT by a random coiled sequence enhanced membrane insertion of Bax. It has previously been suggested that conformational change in the N-terminal end of Bax would allow the C-terminal end to play its anchoring function. We found that a mutant truncated in both N- and C-termini still exhibited a strong binding activity to mitochondria. In mammals, Bax interaction with the caspase-8-generated truncated form of Bid (tc-Bid) is believed to promote a conformational change necessary for the insertion of Bax into mitochondria. We coexpressed Bax and tc-Bid in yeast and found that native Bax functions are not stimulated by tc-Bid, whereas functions of an active variant with a modified CT are. We propose that Bax CT has to undergo a conformational change to allow the insertion of Bax in mitochondria but, contrary to current views, is not a bona fide membrane anchor. | lld:pubmed |
| pubmed-article:12934081 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12934081 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12934081 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12934081 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12934081 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12934081 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12934081 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12934081 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12934081 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12934081 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12934081 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12934081 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12934081 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:12934081 | pubmed:issn | 1350-9047 | lld:pubmed |
| pubmed-article:12934081 | pubmed:author | pubmed-author:AntonssonBrun... | lld:pubmed |
| pubmed-article:12934081 | pubmed:author | pubmed-author:CartronPierre... | lld:pubmed |
| pubmed-article:12934081 | pubmed:author | pubmed-author:ValletteFranç... | lld:pubmed |
| pubmed-article:12934081 | pubmed:author | pubmed-author:ManonStéphenS | lld:pubmed |
| pubmed-article:12934081 | pubmed:author | pubmed-author:CamougrandNad... | lld:pubmed |
| pubmed-article:12934081 | pubmed:author | pubmed-author:PriaultMuriel... | lld:pubmed |
| pubmed-article:12934081 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12934081 | pubmed:volume | 10 | lld:pubmed |
| pubmed-article:12934081 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12934081 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12934081 | pubmed:pagination | 1068-77 | lld:pubmed |
| pubmed-article:12934081 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12934081 | pubmed:meshHeading | pubmed-meshheading:12934081... | lld:pubmed |
| pubmed-article:12934081 | pubmed:meshHeading | pubmed-meshheading:12934081... | lld:pubmed |
| pubmed-article:12934081 | pubmed:meshHeading | pubmed-meshheading:12934081... | lld:pubmed |
| pubmed-article:12934081 | pubmed:meshHeading | pubmed-meshheading:12934081... | lld:pubmed |
| pubmed-article:12934081 | pubmed:meshHeading | pubmed-meshheading:12934081... | lld:pubmed |
| pubmed-article:12934081 | pubmed:meshHeading | pubmed-meshheading:12934081... | lld:pubmed |
| pubmed-article:12934081 | pubmed:meshHeading | pubmed-meshheading:12934081... | lld:pubmed |
| pubmed-article:12934081 | pubmed:meshHeading | pubmed-meshheading:12934081... | lld:pubmed |
| pubmed-article:12934081 | pubmed:meshHeading | pubmed-meshheading:12934081... | lld:pubmed |
| pubmed-article:12934081 | pubmed:meshHeading | pubmed-meshheading:12934081... | lld:pubmed |
| pubmed-article:12934081 | pubmed:meshHeading | pubmed-meshheading:12934081... | lld:pubmed |
| pubmed-article:12934081 | pubmed:meshHeading | pubmed-meshheading:12934081... | lld:pubmed |
| pubmed-article:12934081 | pubmed:meshHeading | pubmed-meshheading:12934081... | lld:pubmed |
| pubmed-article:12934081 | pubmed:meshHeading | pubmed-meshheading:12934081... | lld:pubmed |
| pubmed-article:12934081 | pubmed:meshHeading | pubmed-meshheading:12934081... | lld:pubmed |
| pubmed-article:12934081 | pubmed:meshHeading | pubmed-meshheading:12934081... | lld:pubmed |
| pubmed-article:12934081 | pubmed:meshHeading | pubmed-meshheading:12934081... | lld:pubmed |
| pubmed-article:12934081 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12934081 | pubmed:articleTitle | Investigation of the role of the C-terminus of Bax and of tc-Bid on Bax interaction with yeast mitochondria. | lld:pubmed |
| pubmed-article:12934081 | pubmed:affiliation | UMR 5095 CNRS/Université de Bordeaux 2, 1 Rue Camille Saint-Saëns, F-33077 Bordeaux, France. | lld:pubmed |
| pubmed-article:12934081 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12934081 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12934081 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12934081 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12934081 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12934081 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12934081 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12934081 | lld:pubmed |
