| pubmed-article:12879258 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12879258 | lifeskim:mentions | umls-concept:C0032098 | lld:lifeskim |
| pubmed-article:12879258 | lifeskim:mentions | umls-concept:C0522462 | lld:lifeskim |
| pubmed-article:12879258 | lifeskim:mentions | umls-concept:C0003069 | lld:lifeskim |
| pubmed-article:12879258 | lifeskim:mentions | umls-concept:C0024742 | lld:lifeskim |
| pubmed-article:12879258 | lifeskim:mentions | umls-concept:C0036576 | lld:lifeskim |
| pubmed-article:12879258 | lifeskim:mentions | umls-concept:C1417244 | lld:lifeskim |
| pubmed-article:12879258 | lifeskim:mentions | umls-concept:C1823304 | lld:lifeskim |
| pubmed-article:12879258 | lifeskim:mentions | umls-concept:C1524063 | lld:lifeskim |
| pubmed-article:12879258 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:12879258 | pubmed:dateCreated | 2003-9-23 | lld:pubmed |
| pubmed-article:12879258 | pubmed:abstractText | A new method for obtaining transgenic sweet orange plants was developed in which positive selection (Positech) based on the Escherichia coli phosphomannose-isomerase (PMI) gene as the selectable marker gene and mannose as the selective agent was used. Epicotyl segments from in vitro-germinated plants of Valencia, Hamlin, Natal and Pera sweet oranges were inoculated with Agrobacterium tumefaciens EHA101-pNOV2116 and subsequently selected on medium supplemented with different concentrations of mannose or with a combination of mannose and sucrose as a carbon source. Genetic transformation was confirmed by PCR and Southern blot. The transgene expression was evaluated using a chlorophenol red assay and isoenzymes. The transformation efficiency rate ranged from 3% to 23.8%, depending on cultivar. This system provides an efficient manner for selecting transgenic sweet orange plants without using antibiotics or herbicides. | lld:pubmed |
| pubmed-article:12879258 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12879258 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12879258 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12879258 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12879258 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12879258 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12879258 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12879258 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12879258 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12879258 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:12879258 | pubmed:issn | 0721-7714 | lld:pubmed |
| pubmed-article:12879258 | pubmed:author | pubmed-author:BoscariolR... | lld:pubmed |
| pubmed-article:12879258 | pubmed:author | pubmed-author:AlmeidaW A... | lld:pubmed |
| pubmed-article:12879258 | pubmed:author | pubmed-author:DerbyshireM... | lld:pubmed |
| pubmed-article:12879258 | pubmed:author | pubmed-author:Mourão... | lld:pubmed |
| pubmed-article:12879258 | pubmed:author | pubmed-author:MendesB M JBM | lld:pubmed |
| pubmed-article:12879258 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12879258 | pubmed:volume | 22 | lld:pubmed |
| pubmed-article:12879258 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12879258 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12879258 | pubmed:pagination | 122-8 | lld:pubmed |
| pubmed-article:12879258 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12879258 | pubmed:meshHeading | pubmed-meshheading:12879258... | lld:pubmed |
| pubmed-article:12879258 | pubmed:meshHeading | pubmed-meshheading:12879258... | lld:pubmed |
| pubmed-article:12879258 | pubmed:meshHeading | pubmed-meshheading:12879258... | lld:pubmed |
| pubmed-article:12879258 | pubmed:meshHeading | pubmed-meshheading:12879258... | lld:pubmed |
| pubmed-article:12879258 | pubmed:meshHeading | pubmed-meshheading:12879258... | lld:pubmed |
| pubmed-article:12879258 | pubmed:meshHeading | pubmed-meshheading:12879258... | lld:pubmed |
| pubmed-article:12879258 | pubmed:meshHeading | pubmed-meshheading:12879258... | lld:pubmed |
| pubmed-article:12879258 | pubmed:meshHeading | pubmed-meshheading:12879258... | lld:pubmed |
| pubmed-article:12879258 | pubmed:meshHeading | pubmed-meshheading:12879258... | lld:pubmed |
| pubmed-article:12879258 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12879258 | pubmed:articleTitle | The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck). | lld:pubmed |
| pubmed-article:12879258 | pubmed:affiliation | Laboratório de Biotecnologia Vegetal, Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba 13 400-970 São Paulo, Brazil. | lld:pubmed |
| pubmed-article:12879258 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12879258 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
