| pubmed-article:12877656 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12877656 | lifeskim:mentions | umls-concept:C1383501 | lld:lifeskim |
| pubmed-article:12877656 | lifeskim:mentions | umls-concept:C0806987 | lld:lifeskim |
| pubmed-article:12877656 | lifeskim:mentions | umls-concept:C0007745 | lld:lifeskim |
| pubmed-article:12877656 | lifeskim:mentions | umls-concept:C0031180 | lld:lifeskim |
| pubmed-article:12877656 | lifeskim:mentions | umls-concept:C0441889 | lld:lifeskim |
| pubmed-article:12877656 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:12877656 | lifeskim:mentions | umls-concept:C1517945 | lld:lifeskim |
| pubmed-article:12877656 | pubmed:issue | Pt 3 | lld:pubmed |
| pubmed-article:12877656 | pubmed:dateCreated | 2003-10-22 | lld:pubmed |
| pubmed-article:12877656 | pubmed:abstractText | Ceramide (a sphingolipid) and reactive oxygen species are each partly responsible for intracellular signal transduction in response to a variety of agents. It has been reported that ceramide and reactive oxygen species are intimately linked and show reciprocal regulation [Liu, Andreieu-Abadie, Levade, Zhang, Obeid and Hannun (1998) J. Biol. Chem. 273, 11313-11320]. Utilizing synthetic, short-chain ceramide to mimic the cellular responses to fluctuations in natural endogenous ceramide formation or using stimulation of CD95 to induce ceramide formation, we found that the principal redox-altering property of ceramide is to lower the [peroxide](cyt) (cytosolic peroxide concentration). Apoptosis of Jurkat T-cells, primary resting and phytohaemagglutinin-activated human peripheral blood T-lymphocytes was preceded by a loss in [peroxide](cyt), as measured by the peroxide-sensitive probe 2',7'-dichlorofluorescein diacetate (also reflected in a lower rate of superoxide dismutase-inhibitable cytochrome c reduction), and this was not associated with a loss of membrane integrity. Where growth arrest of U937 monocytes was observed without a loss of membrane integrity, the decrease in [peroxide](cyt) was of a lower magnitude when compared with that preceding the onset of apoptosis in T-cells. Furthermore, decreasing the cytosolic peroxide level in U937 monocytes before the application of synthetic ceramide by pretreatment with either of the antioxidants N -acetyl cysteine or glutathione conferred apoptosis. However, N -acetyl cysteine or glutathione did not affect the kinetics or magnitude of ceramide-induced apoptosis of Jurkat T-cells. Therefore the primary redox effect of cellular ceramide accumulation is to lower the [peroxide](cyt) of both primary and immortalized cells, the magnitude of which dictates the cellular response. | lld:pubmed |
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| pubmed-article:12877656 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12877656 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12877656 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12877656 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12877656 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:12877656 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12877656 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:12877656 | pubmed:issn | 1470-8728 | lld:pubmed |
| pubmed-article:12877656 | pubmed:author | pubmed-author:GriffithsHele... | lld:pubmed |
| pubmed-article:12877656 | pubmed:author | pubmed-author:PhillipsDarre... | lld:pubmed |
| pubmed-article:12877656 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:12877656 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:12877656 | pubmed:volume | 375 | lld:pubmed |
| pubmed-article:12877656 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12877656 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12877656 | pubmed:pagination | 567-79 | lld:pubmed |
| pubmed-article:12877656 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:12877656 | pubmed:meshHeading | pubmed-meshheading:12877656... | lld:pubmed |
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| pubmed-article:12877656 | pubmed:meshHeading | pubmed-meshheading:12877656... | lld:pubmed |
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| pubmed-article:12877656 | pubmed:meshHeading | pubmed-meshheading:12877656... | lld:pubmed |
| pubmed-article:12877656 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12877656 | pubmed:articleTitle | Ceramide induces a loss in cytosolic peroxide levels in mononuclear cells. | lld:pubmed |
| pubmed-article:12877656 | pubmed:affiliation | Molecular Biosciences Group, School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, UK. | lld:pubmed |
| pubmed-article:12877656 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12877656 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12877656 | lld:pubmed |
