pubmed-article:12857854 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:12857854 | lifeskim:mentions | umls-concept:C0521119 | lld:lifeskim |
pubmed-article:12857854 | lifeskim:mentions | umls-concept:C0005758 | lld:lifeskim |
pubmed-article:12857854 | lifeskim:mentions | umls-concept:C0015127 | lld:lifeskim |
pubmed-article:12857854 | lifeskim:mentions | umls-concept:C2936239 | lld:lifeskim |
pubmed-article:12857854 | lifeskim:mentions | umls-concept:C0001480 | lld:lifeskim |
pubmed-article:12857854 | lifeskim:mentions | umls-concept:C1314792 | lld:lifeskim |
pubmed-article:12857854 | lifeskim:mentions | umls-concept:C1522492 | lld:lifeskim |
pubmed-article:12857854 | pubmed:issue | 7 | lld:pubmed |
pubmed-article:12857854 | pubmed:dateCreated | 2003-7-14 | lld:pubmed |
pubmed-article:12857854 | pubmed:abstractText | The P2X7 ATP receptor mediates the cytotoxic effect of extracellular ATP. P2X7-dependent cell death is heralded by dramatic plasma membrane bleb formation. Membrane blebbing is a complex phenomenon involving as yet poorly characterized intracellular pathways. We have investigated the effect of extracellular ATP on HEK293 cells transfected with the cytotoxic/pore-forming P2X7 receptor. Addition of ATP to P2X7-transfected, but not to wt P2X7-less, HEK293 cells caused massive membrane blebbing within 1-2 min. UTP, a nucleotide incapable of activating P2X7, had no early effects on cell shape and bleb formation. Bleb formation triggered by ATP was reversible and required extracellular Ca2+ and an intact cytoskeleton. Furthermore, it was completely prevented by preincubation with the P2X blocker oxidized ATP. It was recently observed that the ROCK protein is a key determinant of bleb formation. Preincubation of HEK293-P2X7 cells with the ROCK blocker Y-27632 completely prevented P2X7-dependent blebbing. Although ATP triggered cleavage of the ROCK I isoform in P2X7-transfected HEK293 cells, the wide range caspase inhibitor z-VAD-fluoromethylketone had no effect. These observations suggest that P2X7-dependent plasma membrane blebbing depends on the activation of the serine/threonine kinase ROCK I. | lld:pubmed |
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pubmed-article:12857854 | pubmed:language | eng | lld:pubmed |
pubmed-article:12857854 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12857854 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:12857854 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:12857854 | pubmed:month | Jul | lld:pubmed |
pubmed-article:12857854 | pubmed:issn | 1059-1524 | lld:pubmed |
pubmed-article:12857854 | pubmed:author | pubmed-author:FalzoniSimone... | lld:pubmed |
pubmed-article:12857854 | pubmed:author | pubmed-author:ChiozziPaolaP | lld:pubmed |
pubmed-article:12857854 | pubmed:author | pubmed-author:MorelliAnnaA | lld:pubmed |
pubmed-article:12857854 | pubmed:author | pubmed-author:Di... | lld:pubmed |
pubmed-article:12857854 | pubmed:author | pubmed-author:FerrariDavide... | lld:pubmed |
pubmed-article:12857854 | pubmed:author | pubmed-author:PintonPaoloP | lld:pubmed |
pubmed-article:12857854 | pubmed:author | pubmed-author:RizzutoRosari... | lld:pubmed |
pubmed-article:12857854 | pubmed:author | pubmed-author:OlsonMichael... | lld:pubmed |
pubmed-article:12857854 | pubmed:author | pubmed-author:ChiesaAnnaA | lld:pubmed |
pubmed-article:12857854 | pubmed:author | pubmed-author:SanzJuana MJM | lld:pubmed |
pubmed-article:12857854 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:12857854 | pubmed:volume | 14 | lld:pubmed |
pubmed-article:12857854 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:12857854 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:12857854 | pubmed:pagination | 2655-64 | lld:pubmed |
pubmed-article:12857854 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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