| pubmed-article:12856169 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12856169 | lifeskim:mentions | umls-concept:C0012634 | lld:lifeskim |
| pubmed-article:12856169 | lifeskim:mentions | umls-concept:C0022650 | lld:lifeskim |
| pubmed-article:12856169 | lifeskim:mentions | umls-concept:C0206745 | lld:lifeskim |
| pubmed-article:12856169 | lifeskim:mentions | umls-concept:C0026336 | lld:lifeskim |
| pubmed-article:12856169 | lifeskim:mentions | umls-concept:C1335132 | lld:lifeskim |
| pubmed-article:12856169 | lifeskim:mentions | umls-concept:C1420135 | lld:lifeskim |
| pubmed-article:12856169 | lifeskim:mentions | umls-concept:C1704827 | lld:lifeskim |
| pubmed-article:12856169 | lifeskim:mentions | umls-concept:C0221099 | lld:lifeskim |
| pubmed-article:12856169 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:12856169 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:12856169 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:12856169 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:12856169 | pubmed:dateCreated | 2003-8-25 | lld:pubmed |
| pubmed-article:12856169 | pubmed:abstractText | The imprinted multimembrane-spanning polyspecific transporter-like gene 1 ( IMPT1) encodes a predicted protein with organic cation transport capabilities. As a first step in understanding the function of IMPT1, we identified the renal structures expressing this gene in knockout mice with adenine phosphoribosyltransferase (APRT) deficiency and 2,8-dihydroxyadenine (DHA) nephrolithiasis. IMPT1 mRNA was not detected using a standard in situ hybridization (ISH) protocol, but we observed intense staining in cortico-medullary tubules and glomeruli in wild-type mice using an improved reverse transcription-polymerase chain reaction (RT-PCR) ISH procedure. IMPT1 mRNA expression was significantly decreased in the cortical region in kidney sections from APRT-deficient male mice. APRT-deficient female mice are less severely affected by DHA-induced kidney stone disease, and we observed only a modest reduction in IMPT1 expression in kidneys from these mice. IMPT1 expression in APRT heterozygous mice was comparable to that in wild-type mice, suggesting imprinting of one of the parental alleles. These findings suggest that decreased IMPT1 mRNA expression may contribute to the impaired renal function in APRT-deficient male mice, and that RT-PCR ISH is a valuable tool for localizing the site of expression of transcripts that are not detectable using standard ISH procedures. | lld:pubmed |
| pubmed-article:12856169 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12856169 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12856169 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12856169 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12856169 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12856169 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12856169 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12856169 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12856169 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12856169 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12856169 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12856169 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12856169 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:12856169 | pubmed:issn | 0300-5623 | lld:pubmed |
| pubmed-article:12856169 | pubmed:author | pubmed-author:FassR JRJ | lld:pubmed |
| pubmed-article:12856169 | pubmed:author | pubmed-author:YangMinM | lld:pubmed |
| pubmed-article:12856169 | pubmed:author | pubmed-author:EvanAndrew... | lld:pubmed |
| pubmed-article:12856169 | pubmed:author | pubmed-author:TischfieldJay... | lld:pubmed |
| pubmed-article:12856169 | pubmed:author | pubmed-author:StambrookPete... | lld:pubmed |
| pubmed-article:12856169 | pubmed:author | pubmed-author:SahotaAmrikA | lld:pubmed |
| pubmed-article:12856169 | pubmed:author | pubmed-author:TzortzakiElen... | lld:pubmed |
| pubmed-article:12856169 | pubmed:author | pubmed-author:GlassDaynaD | lld:pubmed |
| pubmed-article:12856169 | pubmed:author | pubmed-author:BledsoeSharon... | lld:pubmed |
| pubmed-article:12856169 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12856169 | pubmed:volume | 31 | lld:pubmed |
| pubmed-article:12856169 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12856169 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12856169 | pubmed:pagination | 257-61 | lld:pubmed |
| pubmed-article:12856169 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:12856169 | pubmed:meshHeading | pubmed-meshheading:12856169... | lld:pubmed |
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| pubmed-article:12856169 | pubmed:meshHeading | pubmed-meshheading:12856169... | lld:pubmed |
| pubmed-article:12856169 | pubmed:meshHeading | pubmed-meshheading:12856169... | lld:pubmed |
| pubmed-article:12856169 | pubmed:meshHeading | pubmed-meshheading:12856169... | lld:pubmed |
| pubmed-article:12856169 | pubmed:meshHeading | pubmed-meshheading:12856169... | lld:pubmed |
| pubmed-article:12856169 | pubmed:meshHeading | pubmed-meshheading:12856169... | lld:pubmed |
| pubmed-article:12856169 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12856169 | pubmed:articleTitle | Impaired expression of an organic cation transporter, IMPT1, in a knockout mouse model for kidney stone disease. | lld:pubmed |
| pubmed-article:12856169 | pubmed:affiliation | Department of Genetics, Rutgers University, 604 Allison Road, NJ 08854-8082, Piscataway, USA. | lld:pubmed |
| pubmed-article:12856169 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12856169 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12856169 | lld:pubmed |
