| pubmed-article:12827307 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12827307 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
| pubmed-article:12827307 | lifeskim:mentions | umls-concept:C1513095 | lld:lifeskim |
| pubmed-article:12827307 | lifeskim:mentions | umls-concept:C0003968 | lld:lifeskim |
| pubmed-article:12827307 | lifeskim:mentions | umls-concept:C1704259 | lld:lifeskim |
| pubmed-article:12827307 | lifeskim:mentions | umls-concept:C1705987 | lld:lifeskim |
| pubmed-article:12827307 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:12827307 | lifeskim:mentions | umls-concept:C0591833 | lld:lifeskim |
| pubmed-article:12827307 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:12827307 | pubmed:dateCreated | 2003-10-2 | lld:pubmed |
| pubmed-article:12827307 | pubmed:abstractText | L-ascorbic acid (vitamin C) has been reported to play a role in the treatment and prevention of cancer. However, its specific mechanistic pathways remain obscure. This study was carried out to identify the sodium ascorbate-induced apoptotic pathway in B16F10 murine melanoma cells. Sodium ascorbate was found to induce the apoptosis of B16F10 murine melanoma in a time- and dose-dependent manner, and this was prevented by pretreatment with N-acetyl- L-cysteine (NAC), a well-known antioxidant. In fact, sodium ascorbate-treated B16F10 melanoma cells showed increased intracellular reactive oxygen species generation (ROS) levels. These results indicate that sodium ascorbate induced apoptosis in B16F10 murine melanoma cells by acting as a prooxidant. We examined the involvement of caspase-8 using a specific caspase-8 inhibitor (z-IETD-fmk) on the sodium ascorbate-induced apoptotic pathway. Cell death was found not to be inhibited by z-IETD-fmk treatment, indicating that sodium ascorbate-induced apoptosis is not mediated by caspase-8. In addition, we detected a reduction in the mitochondrial membrane potential during apoptosis and confirmed cytochrome-c release from mitochondria by immunoblotting. Taken together, it appears that the induction of a prooxidant state by sodium ascorbate and a subsequent reduction in mitochondrial membrane potential are involved in the apoptotic pathway of B16F10 murine melanoma cells, and that this occurs in a caspase-8-independent manner. | lld:pubmed |
| pubmed-article:12827307 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12827307 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12827307 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12827307 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12827307 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12827307 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12827307 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12827307 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12827307 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12827307 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12827307 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12827307 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12827307 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12827307 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:12827307 | pubmed:issn | 0340-7004 | lld:pubmed |
| pubmed-article:12827307 | pubmed:author | pubmed-author:LeeWang JaeWJ | lld:pubmed |
| pubmed-article:12827307 | pubmed:author | pubmed-author:ChoDaehoD | lld:pubmed |
| pubmed-article:12827307 | pubmed:author | pubmed-author:KimYoung-InYI | lld:pubmed |
| pubmed-article:12827307 | pubmed:author | pubmed-author:KimDaejinD | lld:pubmed |
| pubmed-article:12827307 | pubmed:author | pubmed-author:KangJae... | lld:pubmed |
| pubmed-article:12827307 | pubmed:author | pubmed-author:HahmEunsilE | lld:pubmed |
| pubmed-article:12827307 | pubmed:author | pubmed-author:YangYoolheeY | lld:pubmed |
| pubmed-article:12827307 | pubmed:author | pubmed-author:ParkHyunjeong... | lld:pubmed |
| pubmed-article:12827307 | pubmed:author | pubmed-author:HurDaeyoungD | lld:pubmed |
| pubmed-article:12827307 | pubmed:author | pubmed-author:BangSaicS | lld:pubmed |
| pubmed-article:12827307 | pubmed:author | pubmed-author:HwangYoung... | lld:pubmed |
| pubmed-article:12827307 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12827307 | pubmed:volume | 52 | lld:pubmed |
| pubmed-article:12827307 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12827307 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12827307 | pubmed:pagination | 693-8 | lld:pubmed |
| pubmed-article:12827307 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12827307 | pubmed:meshHeading | pubmed-meshheading:12827307... | lld:pubmed |
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| pubmed-article:12827307 | pubmed:meshHeading | pubmed-meshheading:12827307... | lld:pubmed |
| pubmed-article:12827307 | pubmed:meshHeading | pubmed-meshheading:12827307... | lld:pubmed |
| pubmed-article:12827307 | pubmed:meshHeading | pubmed-meshheading:12827307... | lld:pubmed |
| pubmed-article:12827307 | pubmed:meshHeading | pubmed-meshheading:12827307... | lld:pubmed |
| pubmed-article:12827307 | pubmed:meshHeading | pubmed-meshheading:12827307... | lld:pubmed |
| pubmed-article:12827307 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12827307 | pubmed:articleTitle | L-ascorbic acid (vitamin C) induces the apoptosis of B16 murine melanoma cells via a caspase-8-independent pathway. | lld:pubmed |
| pubmed-article:12827307 | pubmed:affiliation | Department of Anatomy and Tumor Immunity Medical Research Center, Seoul National University College of Medicine, 110-799 Seoul, South Korea. | lld:pubmed |
| pubmed-article:12827307 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12827307 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12827307 | lld:pubmed |
