pubmed-article:12827219 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:12827219 | lifeskim:mentions | umls-concept:C0521009 | lld:lifeskim |
pubmed-article:12827219 | lifeskim:mentions | umls-concept:C0699900 | lld:lifeskim |
pubmed-article:12827219 | lifeskim:mentions | umls-concept:C0243125 | lld:lifeskim |
pubmed-article:12827219 | lifeskim:mentions | umls-concept:C1311768 | lld:lifeskim |
pubmed-article:12827219 | lifeskim:mentions | umls-concept:C0052420 | lld:lifeskim |
pubmed-article:12827219 | pubmed:issue | 2 | lld:pubmed |
pubmed-article:12827219 | pubmed:dateCreated | 2003-8-4 | lld:pubmed |
pubmed-article:12827219 | pubmed:abstractText | Microorganisms from Mytilus edulis (marine mussel) degraded arsenobetaine, with the formation of trimethylarsine oxide, dimethylarsinate and methylarsonate. Four bacterial isolates from these mixed-cultures were shown by HPLC/hydride generation-atomic fluorescence spectroscopy (HPLC/HG-AFS) analysis to degrade arsenobetaine to dimethylarsinate in pure culture; there was no evidence of trimethylarsine oxide formation. Two of the isolates ( Paenibacillussp. strain 13943 and Pseudomonas sp. strain 13944) were shown by HPLC/inductively coupled plasma-mass spectrometry (HPLC/ICPMS) analysis to degrade arsenobetaine by initial cleavage of a methyl-arsenic bond to form dimethylarsinoylacetate, with subsequent cleavage of the carboxymethyl-arsenic bond to yield dimethylarsinate. Arsenobetaine biodegradation by pure cultures was biphasic, with dimethylarsinoylacetate accumulating in culture supernatants during the culture growth phase and its removal accompanying dimethylarsinate formation during a carbon-limited stationary phase. The Paenibacillus sp. also converted exogenously supplied dimethylarsinoylacetate to dimethylarsinate only under carbon-limited conditions. Lysed-cell extracts of the Paenibacillus sp. showed constitutive expression of enzyme(s) capable of arsenobetaine degradation through methyl-arsenic and carboxymethyl-arsenic bond cleavage. The work establishes the capability of particular bacteria to cleave both types of arsenic-carbon bonds of arsenobetaine and demonstrates that mixed-community functioning is not an obligate requirement for arsenobetaine biodegradation. | lld:pubmed |
pubmed-article:12827219 | pubmed:language | eng | lld:pubmed |
pubmed-article:12827219 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12827219 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:12827219 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12827219 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12827219 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12827219 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12827219 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:12827219 | pubmed:month | Aug | lld:pubmed |
pubmed-article:12827219 | pubmed:issn | 0302-8933 | lld:pubmed |
pubmed-article:12827219 | pubmed:author | pubmed-author:GoesslerWalte... | lld:pubmed |
pubmed-article:12827219 | pubmed:author | pubmed-author:EdmondsJohn... | lld:pubmed |
pubmed-article:12827219 | pubmed:author | pubmed-author:JenkinsRichar... | lld:pubmed |
pubmed-article:12827219 | pubmed:author | pubmed-author:KuehneltDoris... | lld:pubmed |
pubmed-article:12827219 | pubmed:author | pubmed-author:RitchieAlisda... | lld:pubmed |
pubmed-article:12827219 | pubmed:author | pubmed-author:MolenatNathal... | lld:pubmed |
pubmed-article:12827219 | pubmed:author | pubmed-author:HarringtonChr... | lld:pubmed |
pubmed-article:12827219 | pubmed:author | pubmed-author:SuttonPeter... | lld:pubmed |
pubmed-article:12827219 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:12827219 | pubmed:volume | 180 | lld:pubmed |
pubmed-article:12827219 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:12827219 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:12827219 | pubmed:pagination | 142-50 | lld:pubmed |
pubmed-article:12827219 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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pubmed-article:12827219 | pubmed:year | 2003 | lld:pubmed |
pubmed-article:12827219 | pubmed:articleTitle | Bacterial degradation of arsenobetaine via dimethylarsinoylacetate. | lld:pubmed |
pubmed-article:12827219 | pubmed:affiliation | School of Molecular Science, De Montfort University, The Gateway, LE1 9BH, Leicester, UK. roj@dmu.ac.uk | lld:pubmed |
pubmed-article:12827219 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:12827219 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |