| pubmed-article:12819078 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12819078 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:12819078 | lifeskim:mentions | umls-concept:C0035584 | lld:lifeskim |
| pubmed-article:12819078 | lifeskim:mentions | umls-concept:C1332717 | lld:lifeskim |
| pubmed-article:12819078 | lifeskim:mentions | umls-concept:C1706438 | lld:lifeskim |
| pubmed-article:12819078 | lifeskim:mentions | umls-concept:C0085358 | lld:lifeskim |
| pubmed-article:12819078 | lifeskim:mentions | umls-concept:C0282580 | lld:lifeskim |
| pubmed-article:12819078 | lifeskim:mentions | umls-concept:C1413244 | lld:lifeskim |
| pubmed-article:12819078 | lifeskim:mentions | umls-concept:C2698600 | lld:lifeskim |
| pubmed-article:12819078 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:12819078 | pubmed:dateCreated | 2003-6-23 | lld:pubmed |
| pubmed-article:12819078 | pubmed:abstractText | The 1.2-kb DNA fragment of the Rickettsia conorii outer membrane protein B gene (OmpB(451-846)) was subcloned using site-specific PCR primers and expressed as six smaller fragments: OmpB(458-652), OmpB(595-744), OmpB(595-654), OmpB(645-692), OmpB(689-744), and OmpB(739-848). NCTC cells transfected with a mammalian expression vector expressing the fragments OmpB(689-744) and OmpB(739-848) stimulated immune anti-R. conorii CD8 T lymphocytes, suggesting the presence of CD8 T-lymphocyte-stimulating epitopes on these fragments. In order to further characterize the CD8 T-lymphocyte-stimulatory elements, CD8 T-lymphocyte epitopes on OmpB(689-744) and OmpB(739-848) were mapped by overlapping synthetic peptides. The ability of these synthetic peptides to stimulate immune CD8 T lymphocytes was determined by gamma interferon (IFN-gamma) production and cell proliferation after incubation with simian virus 40-transformed murine vascular endothelial cells in the presence of a 20 micro M solution of each synthetic peptide. Five synthetic peptides, SKGVNVDTV (OmpB(708-716)), ANVGSFVFN (OmpB(735-743)), IVSGTVGGQ (OmpB(749-757)), ANSTLQIGG (OmpB(789-797)), and IVEFVNTGP (OmpB(812-820)), induced secretion of IFN-gamma at significantly higher levels than the controls. Three of these five peptides, SKGVNVDTV (OmpB(708-716)), ANSTLQIGG (OmpB(789-797)), and IVEFVNTGP (OmpB(812-820)), also stimulated the proliferation of immune CD8 T lymphocytes. Significantly higher levels of specific cytotoxic T-lymphocyte killing were observed with the same three synthetic peptides, SKGVNVDTV (OmpB(708-716)), ANSTLQIGG (OmpB(789-797)), and IVEFVNTGP (OmpB(812-820)). | lld:pubmed |
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| pubmed-article:12819078 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12819078 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12819078 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:12819078 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12819078 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:12819078 | pubmed:issn | 0019-9567 | lld:pubmed |
| pubmed-article:12819078 | pubmed:author | pubmed-author:WalkerDavid... | lld:pubmed |
| pubmed-article:12819078 | pubmed:author | pubmed-author:YuXue-JieXJ | lld:pubmed |
| pubmed-article:12819078 | pubmed:author | pubmed-author:LiZhenZ | lld:pubmed |
| pubmed-article:12819078 | pubmed:author | pubmed-author:OlanoJuan PJP | lld:pubmed |
| pubmed-article:12819078 | pubmed:author | pubmed-author:FengHui-MinHM | lld:pubmed |
| pubmed-article:12819078 | pubmed:author | pubmed-author:ValbuenaGusta... | lld:pubmed |
| pubmed-article:12819078 | pubmed:author | pubmed-author:Díaz-MonteroC... | lld:pubmed |
| pubmed-article:12819078 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12819078 | pubmed:volume | 71 | lld:pubmed |
| pubmed-article:12819078 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12819078 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12819078 | pubmed:pagination | 3920-6 | lld:pubmed |
| pubmed-article:12819078 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:12819078 | pubmed:meshHeading | pubmed-meshheading:12819078... | lld:pubmed |
| pubmed-article:12819078 | pubmed:meshHeading | pubmed-meshheading:12819078... | lld:pubmed |
| pubmed-article:12819078 | pubmed:meshHeading | pubmed-meshheading:12819078... | lld:pubmed |
| pubmed-article:12819078 | pubmed:meshHeading | pubmed-meshheading:12819078... | lld:pubmed |
| pubmed-article:12819078 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12819078 | pubmed:articleTitle | Identification of CD8 T-lymphocyte epitopes in OmpB of Rickettsia conorii. | lld:pubmed |
| pubmed-article:12819078 | pubmed:affiliation | Department of Pathology, WHO Collaborating Center for Tropical Diseases, University of Texas Medical Branch at Galveston, Galveston, Texas 77555-0609, USA. | lld:pubmed |
| pubmed-article:12819078 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12819078 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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