| pubmed-article:12814345 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12814345 | lifeskim:mentions | umls-concept:C1123019 | lld:lifeskim |
| pubmed-article:12814345 | lifeskim:mentions | umls-concept:C0036536 | lld:lifeskim |
| pubmed-article:12814345 | lifeskim:mentions | umls-concept:C0036537 | lld:lifeskim |
| pubmed-article:12814345 | lifeskim:mentions | umls-concept:C0032005 | lld:lifeskim |
| pubmed-article:12814345 | lifeskim:mentions | umls-concept:C0227893 | lld:lifeskim |
| pubmed-article:12814345 | lifeskim:mentions | umls-concept:C1521761 | lld:lifeskim |
| pubmed-article:12814345 | lifeskim:mentions | umls-concept:C0392756 | lld:lifeskim |
| pubmed-article:12814345 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:12814345 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:12814345 | pubmed:dateCreated | 2003-6-19 | lld:pubmed |
| pubmed-article:12814345 | pubmed:abstractText | The decline in pulsatile LH secretion and pituitary responsiveness to GnRH as pregnancy advances may be due to non-steroidal factors secreted by the ovine corpus luteum of pregnancy. Corpora lutea from ten ewes on days 70-80 of gestation were homogenized, charcoal-treated and, together with charcoal-treated follicular fluid from superovulated women, were subjected to inhibin immunoaffinity chromatography, reducing dimeric inhibin A and B by >90% and abolishing inhibin bioactivity. These preparations were investigated using cultures of rat pituitary cells. GnRH-induced LH and FSH secretion in vitro was reduced by ovine corpus luteum extract and human follicular fluid by 47+/-5% and 42+/-5% of control LH and by 37+/-5% and 50+/-10% of control FSH, respectively (P<0.001). Extracts prepared from corpora lutea and placentae that were collected on days 50, 90 and 120 of pregnancy (five ewes per stage of pregnancy) showed increased GnRH-induced LH-suppressing bioactivity, particularly in the case of the placental extracts, with a threefold increase in activity. When partially purified by pseudochromatofocusing, GnRH-induced LH-suppressing bioactivity in extracts of ovine corpora lutea was identified at pH 5.40 and 5.77. Although these values are similar to published gonadotrophin surge-attenuating factor (GnSAF) bioactivity pI values, a GnSAF-blocking antiserum had no consistent effect on ovine corpus luteum extract GnRH-induced LH-suppressing bioactivity. It was concluded that the ovine corpus luteum of pregnancy contains a non-steroidal, non-inhibin factor, probably not GnSAF, that has the ability to reduce pituitary responsiveness to GnRH in vitro. | lld:pubmed |
| pubmed-article:12814345 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12814345 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12814345 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12814345 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12814345 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12814345 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12814345 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12814345 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12814345 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12814345 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12814345 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12814345 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12814345 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:12814345 | pubmed:issn | 1470-1626 | lld:pubmed |
| pubmed-article:12814345 | pubmed:author | pubmed-author:GroomeN PNP | lld:pubmed |
| pubmed-article:12814345 | pubmed:author | pubmed-author:FowlerP APA | lld:pubmed |
| pubmed-article:12814345 | pubmed:author | pubmed-author:Al-GuboryK... | lld:pubmed |
| pubmed-article:12814345 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12814345 | pubmed:volume | 126 | lld:pubmed |
| pubmed-article:12814345 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12814345 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12814345 | pubmed:pagination | 35-42 | lld:pubmed |
| pubmed-article:12814345 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12814345 | pubmed:meshHeading | pubmed-meshheading:12814345... | lld:pubmed |
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| pubmed-article:12814345 | pubmed:meshHeading | pubmed-meshheading:12814345... | lld:pubmed |
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| pubmed-article:12814345 | pubmed:meshHeading | pubmed-meshheading:12814345... | lld:pubmed |
| pubmed-article:12814345 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12814345 | pubmed:articleTitle | Demonstration of a non-steroidal, non-inhibin factor in the ovine corpus luteum of pregnancy that reduces pituitary responsiveness to GnRH-induced LH secretion in vitro. | lld:pubmed |
| pubmed-article:12814345 | pubmed:affiliation | Department of Obstetrics and Gynaecology, University of Aberdeen, Aberdeen AB25 2ZD, UK. p.a.fowler@abdn.ac.uk | lld:pubmed |
| pubmed-article:12814345 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12814345 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
