| pubmed-article:1281309 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1281309 | lifeskim:mentions | umls-concept:C0282639 | lld:lifeskim |
| pubmed-article:1281309 | lifeskim:mentions | umls-concept:C0001455 | lld:lifeskim |
| pubmed-article:1281309 | lifeskim:mentions | umls-concept:C0022023 | lld:lifeskim |
| pubmed-article:1281309 | lifeskim:mentions | umls-concept:C0038333 | lld:lifeskim |
| pubmed-article:1281309 | lifeskim:mentions | umls-concept:C0055363 | lld:lifeskim |
| pubmed-article:1281309 | lifeskim:mentions | umls-concept:C0241938 | lld:lifeskim |
| pubmed-article:1281309 | lifeskim:mentions | umls-concept:C0312418 | lld:lifeskim |
| pubmed-article:1281309 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:1281309 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:1281309 | lifeskim:mentions | umls-concept:C0036667 | lld:lifeskim |
| pubmed-article:1281309 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:1281309 | pubmed:dateCreated | 1993-1-11 | lld:pubmed |
| pubmed-article:1281309 | pubmed:abstractText | Previous studies in HT29 cells utilizing the cell-attached nystatin (CAN) method [Greger R, Kunzelmann K (1991) Pflügers Arch 419:209-211] have revealed that the Cl- channels induced by cAMP or by increasing cytosolic Ca2+, e.g. by addition of ATP, and by hypotonic cell swelling share in common their conductance, which was so small in our studies [Kunzelmann et al. (1992) Pflügers Arch (in press)] that we could not resolve it at the single-channel level. This prompted the question whether these Cl- conductances can be distinguished in terms of their ion selectivity and sensitivity towards inhibitors. Whether these pathways are additive or not was also examined. The present study utilized the whole-cell patch-clamp and the CAN methods. A total of 160 patches were studied. In whole-cell patches 8-(4-chlorophenylthio)-cAMP (cAMP, 0.1 +/- 1 mmol/l) induced a significant depolarization by 5 mV and a twofold increase in conductance (G) from 6.2 +/- 1.5 nS to 11.7 +/- 3.2 nS (n = 15). Total replacement of Cl- by Br- and I- in cAMP-treated cells hyperpolarized the membrane voltage (V) significantly from -35 +/- 2.8 to -39 +/- 3.4 and -45 +/- 3.3 mV respectively, but had no detectable effect on G, which was 11.9 +/- 3.3 nS in the case of Br- and 11.8 +/- 3.3 nS in the case of I-. Hence, the permselectivity of the cAMP pathway was I- > Br- > Cl-, but the conductances for these anions were all indistinguishable.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:1281309 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1281309 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1281309 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1281309 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1281309 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:1281309 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1281309 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1281309 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:1281309 | pubmed:issn | 0031-6768 | lld:pubmed |
| pubmed-article:1281309 | pubmed:author | pubmed-author:GregerRR | lld:pubmed |
| pubmed-article:1281309 | pubmed:author | pubmed-author:WarthRR | lld:pubmed |
| pubmed-article:1281309 | pubmed:author | pubmed-author:AllersMM | lld:pubmed |
| pubmed-article:1281309 | pubmed:author | pubmed-author:KubitzRR | lld:pubmed |
| pubmed-article:1281309 | pubmed:author | pubmed-author:KunzelmannKK | lld:pubmed |
| pubmed-article:1281309 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1281309 | pubmed:volume | 421 | lld:pubmed |
| pubmed-article:1281309 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1281309 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1281309 | pubmed:pagination | 447-54 | lld:pubmed |
| pubmed-article:1281309 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:1281309 | pubmed:meshHeading | pubmed-meshheading:1281309-... | lld:pubmed |
| pubmed-article:1281309 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1281309 | pubmed:articleTitle | Small-conductance chloride channels induced by cAMP, Ca2+, and hypotonicity in HT29 cells: ion selectivity, additivity and stilbene sensitivity. | lld:pubmed |
| pubmed-article:1281309 | pubmed:affiliation | Physiologisches Institut der Albert-Ludwigs-Universität Freiburg, Federal Republic of Germany. | lld:pubmed |
| pubmed-article:1281309 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1281309 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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