Linked Life Data

12810045

Source:http://linkedlifedata.com/resource/pubmed/id/12810045

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pubmed-article:12810045pubmed:dateCreated2003-6-17lld:pubmed
pubmed-article:12810045pubmed:abstractTextSome isolates of Trichomonas vaginalis, the number one, non-viral sexually transmitted disease agent, are infected with one or several distinct double stranded (ds)-RNA virus. Immune rabbit anti-capsid serum (IRS) reacted with the capsid protein of purified dsRNA virus of a subset of the virus-infected T. vaginalis isolates. A monoclonal antibody (mAb) that recognized the capsid protein reactive with the IRS was generated. Analysis of the virus capsid protein of virus-infected isolates by probing nitrocellulose blots with mAb revealed diversity among immunoreactivity and in the size of the reactive capsid protein. Despite difficulties in visualizing virus within parasites by cross-section electron microscopy, gold-conjugated mAb readily labeled the cytoplasm of virus-positive trichomonads. Finally and importantly, isolates infecting patients attending an STD clinic, 75% of which were virus-positive isolates, had capsid protein of the same size detected by mAb present in all dsRNA viruses.lld:pubmed
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pubmed-article:12810045pubmed:authorpubmed-author:AldereteJ FJFlld:pubmed
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pubmed-article:12810045pubmed:volume103lld:pubmed
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pubmed-article:12810045pubmed:pagination44-50lld:pubmed
pubmed-article:12810045pubmed:dateRevised2008-10-9lld:pubmed
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pubmed-article:12810045pubmed:articleTitleTrichomonas vaginalis: evaluating capsid proteins of dsRNA viruses and the dsRNA virus within patients attending a sexually transmitted disease clinic.lld:pubmed
pubmed-article:12810045pubmed:affiliationDepartment of Microbiology, University of Texas Health Science Center, MC7758, 7703 Floyd Curl Dr., San Antonio, TX 78229-3900, USA. alderete@uthscsa.edulld:pubmed
pubmed-article:12810045pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:12810045pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:12810045pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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