pubmed-article:12771230 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:12771230 | lifeskim:mentions | umls-concept:C0026032 | lld:lifeskim |
pubmed-article:12771230 | lifeskim:mentions | umls-concept:C1330957 | lld:lifeskim |
pubmed-article:12771230 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
pubmed-article:12771230 | lifeskim:mentions | umls-concept:C0017428 | lld:lifeskim |
pubmed-article:12771230 | lifeskim:mentions | umls-concept:C0443286 | lld:lifeskim |
pubmed-article:12771230 | lifeskim:mentions | umls-concept:C0205281 | lld:lifeskim |
pubmed-article:12771230 | lifeskim:mentions | umls-concept:C0752046 | lld:lifeskim |
pubmed-article:12771230 | lifeskim:mentions | umls-concept:C1285573 | lld:lifeskim |
pubmed-article:12771230 | lifeskim:mentions | umls-concept:C0596311 | lld:lifeskim |
pubmed-article:12771230 | pubmed:issue | 11 | lld:pubmed |
pubmed-article:12771230 | pubmed:dateCreated | 2003-5-28 | lld:pubmed |
pubmed-article:12771230 | pubmed:abstractText | Here we report proof-of-principle for a microsphere-based genotyping assay that detects single nucleotide polymorphisms (SNPs) directly from human genomic DNA samples. This assay is based on a structure-specific cleavage reaction that achieves single base discrimination with a 5'-nuclease which recognizes a tripartite substrate formed upon hybridization of target DNA with probe and upstream oligonucleotides. The assay is simple with two easy steps: a cleavage reaction, which generates fluorescent signal on microsphere surfaces, followed by flow cytometry analysis of the microspheres. Genomic DNA samples were genotyped for the SNP in the Apolipoprotein E gene at amino acid position 158. The assay successfully scored wild type, heterozygous and homozygous mutants. To our knowledge, this is the first report of a solid-support assay for detection of SNPs directly from genomic DNA without PCR amplification of the target. | lld:pubmed |
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pubmed-article:12771230 | pubmed:language | eng | lld:pubmed |
pubmed-article:12771230 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12771230 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:12771230 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:12771230 | pubmed:month | Jun | lld:pubmed |
pubmed-article:12771230 | pubmed:issn | 1362-4962 | lld:pubmed |
pubmed-article:12771230 | pubmed:author | pubmed-author:HallJeff GJG | lld:pubmed |
pubmed-article:12771230 | pubmed:author | pubmed-author:StevensPrisci... | lld:pubmed |
pubmed-article:12771230 | pubmed:author | pubmed-author:KelsoDavid... | lld:pubmed |
pubmed-article:12771230 | pubmed:author | pubmed-author:LyamichevVict... | lld:pubmed |
pubmed-article:12771230 | pubmed:author | pubmed-author:RaoKakuturu... | lld:pubmed |
pubmed-article:12771230 | pubmed:author | pubmed-author:NeriBruce PBP | lld:pubmed |
pubmed-article:12771230 | pubmed:issnType | Electronic | lld:pubmed |
pubmed-article:12771230 | pubmed:day | 1 | lld:pubmed |
pubmed-article:12771230 | pubmed:volume | 31 | lld:pubmed |
pubmed-article:12771230 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:12771230 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:12771230 | pubmed:pagination | e66 | lld:pubmed |
pubmed-article:12771230 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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pubmed-article:12771230 | pubmed:meshHeading | pubmed-meshheading:12771230... | lld:pubmed |
pubmed-article:12771230 | pubmed:year | 2003 | lld:pubmed |
pubmed-article:12771230 | pubmed:articleTitle | Genotyping single nucleotide polymorphisms directly from genomic DNA by invasive cleavage reaction on microspheres. | lld:pubmed |
pubmed-article:12771230 | pubmed:affiliation | Department of Biomedical Engineering, Robert R. McCormick School of Engineering and Applied Science, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3107, USA. | lld:pubmed |
pubmed-article:12771230 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:12771230 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
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