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pubmed-article:12765521pubmed:abstractTextUsing electromobility shift assay the interaction of fragments of two paralogous rat estrogen sulfotransferase (Ste) genes with proteins of nuclear extracts from male and female rat liver was studied. Male-specific DNA-protein complexes were revealed with labeled oligonucleotides corresponding to fragments +1150/+1449, +1358/+1449, +1397/+1449, and +1417/+1449 of intron 1 of the Ste1 gene. The removal of a 20 bp region corresponding to the sequence +1430/+1449, or even either 5;- or 3;-terminal 5 bp of this region abolished the selective interaction of the oligonucleotides with the male-specific protein(s). According to the results of the experiments on mutual competition of the oligonucleotides, the fragment of the Ste2 gene corresponding to the sequence +1397/+1449 of the Ste1 gene formed complexes with the same male-specific protein(s) as the fragment of the Ste1 gene did. The data suggest the mapped element to participate in gender differentiation of the expression of the Ste1 and Ste2 genes.lld:pubmed
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pubmed-article:12765521pubmed:pagination399-404lld:pubmed
pubmed-article:12765521pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:12765521pubmed:articleTitleLocalization of a site for interaction with hepatic male-specific proteins in two rat estrogen sulfotransferase genes.lld:pubmed
pubmed-article:12765521pubmed:affiliationEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.lld:pubmed
pubmed-article:12765521pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:12765521pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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