Linked Life Data

12723033

Source:http://linkedlifedata.com/resource/pubmed/id/12723033

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pubmed-article:12723033pubmed:abstractTextAn oriented expression library was constructed from the mycelia of the symbiotic model fungus Hebeloma cylindrosporum in the high-level yeast expression vector pDR196. DNA sequencing of approximately 500 expressed sequence tags (ESTs) showed that 15% correspond to known genes, two thirds contain sequences with unknown function, andthe remaining 20% showed no significant similarity to any known genes. The ESTs had a GC content between 44 and 56%, with most of them having a GC content of 52-54%, which could be correlated with GC contents of fungal genes. The library was successfully used to identify the Hebeloma HIS4 gene by functional complementation of a yeast his4 mutant. Thus, the library may serve as a powerful tool for identification and characterization of mycorrhizal genes by EST analysis and for the identification of ectomycorrhizal genes by means of suppression cloning.lld:pubmed
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pubmed-article:12723033pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:12723033pubmed:articleTitleAn expression cDNA library for suppression cloning in yeast mutants, complementation of a yeast his4 mutant, and EST analysis from the symbiotic basidiomycete Hebeloma cylindrosporum.lld:pubmed
pubmed-article:12723033pubmed:affiliationZMBP, Plant Physiology, Auf der Morgenstelle 1, D-72076 Tübingen, Germany. daniel.wipf@zmbp.uni-tuebingen.delld:pubmed
pubmed-article:12723033pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:12723033pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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