| pubmed-article:12718934 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12718934 | lifeskim:mentions | umls-concept:C0431085 | lld:lifeskim |
| pubmed-article:12718934 | lifeskim:mentions | umls-concept:C0439640 | lld:lifeskim |
| pubmed-article:12718934 | lifeskim:mentions | umls-concept:C0301872 | lld:lifeskim |
| pubmed-article:12718934 | lifeskim:mentions | umls-concept:C0302350 | lld:lifeskim |
| pubmed-article:12718934 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:12718934 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:12718934 | pubmed:dateCreated | 2003-4-29 | lld:pubmed |
| pubmed-article:12718934 | pubmed:abstractText | To elicit a therapeutic antitumor immune response, dendritic cells (DCs) have been employed as a cellular adjuvant. Among various DC-based approaches, fusion of DCs and tumor cells potentially confers not only DC functionality, but also a continuous source of unaltered tumor antigens. We have recently demonstrated successful generation of fusion hybrids by a large-scale electrofusion technique. The immunogenicity and therapeutic potential of fusion hybrids were further analyzed in a model system of a murine melanoma cell line expressing beta-galactosidase (beta-gal) as a surrogate tumor antigen. A single vaccination with fusion hybrids plus IL-12 induced a therapeutic immune response against 3-day established pulmonary metastases. This immunotherapy was beta-gal specific and involved both CD4 and CD8 T cells. In vitro, fusion hybrids stimulated specific IFN-gamma secretion from both CD4 and CD8 immune T cells. They also nonspecifically induced IL-10 secretion from CD4 but not CD8 T cells. Compared to other DC loadings, our results demonstrate the superior immunogenicity of fusion. The current technique of electrofusion is adequately developed for clinical use in cancer immunotherapy. | lld:pubmed |
| pubmed-article:12718934 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12718934 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12718934 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12718934 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12718934 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12718934 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12718934 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12718934 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12718934 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12718934 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12718934 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12718934 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12718934 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12718934 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:12718934 | pubmed:issn | 0008-8749 | lld:pubmed |
| pubmed-article:12718934 | pubmed:author | pubmed-author:TillD GDG | lld:pubmed |
| pubmed-article:12718934 | pubmed:author | pubmed-author:TanakaHiroshi... | lld:pubmed |
| pubmed-article:12718934 | pubmed:author | pubmed-author:HayashiTakash... | lld:pubmed |
| pubmed-article:12718934 | pubmed:author | pubmed-author:ShimizuKeijiK | lld:pubmed |
| pubmed-article:12718934 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12718934 | pubmed:volume | 220 | lld:pubmed |
| pubmed-article:12718934 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12718934 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12718934 | pubmed:pagination | 1-12 | lld:pubmed |
| pubmed-article:12718934 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:12718934 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12718934 | pubmed:articleTitle | Therapeutic immune response induced by electrofusion of dendritic and tumor cells. | lld:pubmed |
| pubmed-article:12718934 | pubmed:affiliation | Center for Surgery Research-FF50, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA. | lld:pubmed |
| pubmed-article:12718934 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12718934 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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