| pubmed-article:12677525 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12677525 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:12677525 | lifeskim:mentions | umls-concept:C1681400 | lld:lifeskim |
| pubmed-article:12677525 | lifeskim:mentions | umls-concept:C0282549 | lld:lifeskim |
| pubmed-article:12677525 | lifeskim:mentions | umls-concept:C1136319 | lld:lifeskim |
| pubmed-article:12677525 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:12677525 | lifeskim:mentions | umls-concept:C1511939 | lld:lifeskim |
| pubmed-article:12677525 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:12677525 | pubmed:dateCreated | 2003-4-4 | lld:pubmed |
| pubmed-article:12677525 | pubmed:abstractText | Differentiation therapy for myeloid leukemia offers great potential as a supplement to the current treatment modalities. In the present report, we investigated if the pyranocoumarins, (+/-)-4'- O-acetyl-3'- O-angeloyl- cis-khellactone (or angular pyranocoumarin, APC) isolated from the medicinal plant Peucedanum praeruptorum Dunn, could induce human acute myeloid leukemic HL-60 cells to differentiate and elucidated the molecular mechanism(s) involved. The ability of HL-60 cells to reduce nitroblue tetrazolium (NBT) was significantly increased after APC treatment for 72 h. In these differentiating HL-60 cells, cell surface differentiation markers CD11b (for myeloid cells) and CD14 (for monocytic cells) were detected in 90.3 % and 70.1 % of the cells, respectively. The differentiation inducing effect of APC was time- and dose-dependent. Treatment with 20 microg/mL APC for 72 h inhibited cell growth by 90 % and cell cycle analysis revealed an increase in the proportion of G1 phase cells. In these growth-inhibited cells the expression of the cyclin-dependent kinase inhibitor p27 kip1, but not p21 WAF1, was up-regulated as shown by Western blotting. Differentiation inducing signal pathways were investigated and it was shown that phospho-MEK and phospho-ERK were elevated shortly after the addition of APC. Pre-incubation of the cells with MEK1 inhibitor PD98059 blocked this APC-induced differentiation. Our results suggest that APC are potent inducers of HL-60 cell differentiation along both the myelocytic and monocytic lineages and are potential agents for differentiation-treatment of leukemia. | lld:pubmed |
| pubmed-article:12677525 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12677525 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12677525 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12677525 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12677525 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12677525 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12677525 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12677525 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12677525 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12677525 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12677525 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:12677525 | pubmed:issn | 0032-0943 | lld:pubmed |
| pubmed-article:12677525 | pubmed:author | pubmed-author:YangMengsuM | lld:pubmed |
| pubmed-article:12677525 | pubmed:author | pubmed-author:FongWang-FunW... | lld:pubmed |
| pubmed-article:12677525 | pubmed:author | pubmed-author:ZhangJin-XiaJ... | lld:pubmed |
| pubmed-article:12677525 | pubmed:author | pubmed-author:WuJimmy... | lld:pubmed |
| pubmed-article:12677525 | pubmed:author | pubmed-author:CheungHon-Yeu... | lld:pubmed |
| pubmed-article:12677525 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12677525 | pubmed:volume | 69 | lld:pubmed |
| pubmed-article:12677525 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12677525 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12677525 | pubmed:pagination | 223-9 | lld:pubmed |
| pubmed-article:12677525 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12677525 | pubmed:meshHeading | pubmed-meshheading:12677525... | lld:pubmed |
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| pubmed-article:12677525 | pubmed:meshHeading | pubmed-meshheading:12677525... | lld:pubmed |
| pubmed-article:12677525 | pubmed:meshHeading | pubmed-meshheading:12677525... | lld:pubmed |
| pubmed-article:12677525 | pubmed:meshHeading | pubmed-meshheading:12677525... | lld:pubmed |
| pubmed-article:12677525 | pubmed:meshHeading | pubmed-meshheading:12677525... | lld:pubmed |
| pubmed-article:12677525 | pubmed:meshHeading | pubmed-meshheading:12677525... | lld:pubmed |
| pubmed-article:12677525 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12677525 | pubmed:articleTitle | Pyranocoumarins isolated from Peucedanum praeruptorum as differentiation inducers in human leukemic HL-60 cells. | lld:pubmed |
| pubmed-article:12677525 | pubmed:affiliation | Bioactive Products Research Group, City University of Hong Kong, Kowloon, Hong Kong SAR, China. | lld:pubmed |
| pubmed-article:12677525 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12677525 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
