| pubmed-article:12676721 | rdf:type | pubmed:Citation | lld:pubmed |
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| pubmed-article:12676721 | lifeskim:mentions | umls-concept:C0446125 | lld:lifeskim |
| pubmed-article:12676721 | lifeskim:mentions | umls-concept:C0162741 | lld:lifeskim |
| pubmed-article:12676721 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:12676721 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
| pubmed-article:12676721 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:12676721 | lifeskim:mentions | umls-concept:C0079429 | lld:lifeskim |
| pubmed-article:12676721 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
| pubmed-article:12676721 | lifeskim:mentions | umls-concept:C0679058 | lld:lifeskim |
| pubmed-article:12676721 | lifeskim:mentions | umls-concept:C1547699 | lld:lifeskim |
| pubmed-article:12676721 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:12676721 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:12676721 | pubmed:dateCreated | 2003-4-4 | lld:pubmed |
| pubmed-article:12676721 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12676721 | pubmed:abstractText | Alternaria species are considered some of the most important fungi responsible for allergenic morbidity in humans. The Alternaria protein that elicits the most intense allergic reaction in humans is Alt a 1, yet no biological function has been identified for this protein. In this study, suppression subtractive hybridization and virtual Northern blots were used to identify and characterize an Alt a 1 homolog in the phytopathogenic fungus Alternaria brassicicola. RNA was extracted from A. brassicicola spores germinated in water and on leaf surfaces of the Arabidopsis ecotype Landsberg for 24 h and used to create cDNA by PCR. Double-stranded cDNA was then used in suppression subtractive hybridization to identify differentially expressed genes. mRNA transcript levels were assessed by virtual Northern blotting. A sequence with significant homology (90% amino acid, 92% cDNA) to the Alt a 1 subunit from Alternaria alternata was identified. Virtual Northern blots demonstrated that this homolog, designated Alt b 1 precursor, was highly up-regulated during the infection process of A. brassicicola on Arabidopsis: The full-length cDNA sequence of Alt b 1 was 815 bp, with an open reading frame of 477 bp. In this preliminary study, we identified a homolog of the major Alternaria allergen precursor, Alt a 1, in A. brassicicola, designated Alt b 1. This isoallergen is differentially expressed during fungal pathogenesis on Arabidopsis, suggesting a possible biological role in pathogenesis. | lld:pubmed |
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| pubmed-article:12676721 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12676721 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12676721 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:12676721 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12676721 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:12676721 | pubmed:issn | 0099-2240 | lld:pubmed |
| pubmed-article:12676721 | pubmed:author | pubmed-author:LawrenceChris... | lld:pubmed |
| pubmed-article:12676721 | pubmed:author | pubmed-author:CramerRobert... | lld:pubmed |
| pubmed-article:12676721 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12676721 | pubmed:volume | 69 | lld:pubmed |
| pubmed-article:12676721 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12676721 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12676721 | pubmed:pagination | 2361-4 | lld:pubmed |
| pubmed-article:12676721 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:12676721 | pubmed:meshHeading | pubmed-meshheading:12676721... | lld:pubmed |
| pubmed-article:12676721 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12676721 | pubmed:articleTitle | Cloning of a gene encoding an Alt a 1 isoallergen differentially expressed by the necrotrophic fungus Alternaria brassicicola during Arabidopsis infection. | lld:pubmed |
| pubmed-article:12676721 | pubmed:affiliation | Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins 80523-1177, USA. | lld:pubmed |
| pubmed-article:12676721 | pubmed:publicationType | Journal Article | lld:pubmed |
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