| pubmed-article:12659833 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12659833 | lifeskim:mentions | umls-concept:C0026030 | lld:lifeskim |
| pubmed-article:12659833 | lifeskim:mentions | umls-concept:C1882726 | lld:lifeskim |
| pubmed-article:12659833 | lifeskim:mentions | umls-concept:C0014417 | lld:lifeskim |
| pubmed-article:12659833 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:12659833 | lifeskim:mentions | umls-concept:C0025513 | lld:lifeskim |
| pubmed-article:12659833 | lifeskim:mentions | umls-concept:C0720298 | lld:lifeskim |
| pubmed-article:12659833 | lifeskim:mentions | umls-concept:C0046439 | lld:lifeskim |
| pubmed-article:12659833 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:12659833 | pubmed:dateCreated | 2003-3-27 | lld:pubmed |
| pubmed-article:12659833 | pubmed:abstractText | In this study, the metabolic activation of 2-nitrofluorene (NF) to estrogenic compounds was examined. NF was negative in estrogen reporter assays using estrogen-responsive yeast and human breast cancer cell line MCF-7. However, the compound exhibited estrogenic activity after incubation with liver microsomes of 3-methylcholanthrene-treated rats in the presence of NADPH. Minor estrogenic activity was observed when liver microsomes of untreated or phenobarbital-treated rats were used instead of those from 3-methylcholanthrene-treated rats. When the compound was incubated with the liver microsomes of 3-methylcholanthrene-treated rats in the presence of NADPH, 7-hydroxy-2-nitrofluorene (7-OH-NF) was formed as a major metabolite. However, little of the metabolite was formed by liver microsomes of untreated or phenobarbital-treated rats. Rat recombinant cytochrome P450 1A1 exhibited a significant oxidase activity toward NF, affording 7-OH-NF. Liver microsomes of phenobarbital-treated rats also enhanced oxidase activity toward NF. In this case, 9-hydroxy-2-nitrofluorene was formed. 7-OH-NF exhibited a significant estrogenic activity, while the activity of 9-hydroxy-2-nitrofluorene was much lower. These results suggest that the estrogenic activity of NF was due to formation of the 7-hydroxylated metabolite by liver microsomes. | lld:pubmed |
| pubmed-article:12659833 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12659833 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12659833 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12659833 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12659833 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12659833 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12659833 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12659833 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12659833 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12659833 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12659833 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:12659833 | pubmed:issn | 0006-291X | lld:pubmed |
| pubmed-article:12659833 | pubmed:author | pubmed-author:KitamuraShige... | lld:pubmed |
| pubmed-article:12659833 | pubmed:author | pubmed-author:SugiharaKazum... | lld:pubmed |
| pubmed-article:12659833 | pubmed:author | pubmed-author:OhtaShigeruS | lld:pubmed |
| pubmed-article:12659833 | pubmed:author | pubmed-author:SanohSeigoS | lld:pubmed |
| pubmed-article:12659833 | pubmed:author | pubmed-author:FujimotoTakas... | lld:pubmed |
| pubmed-article:12659833 | pubmed:author | pubmed-author:FujimotoNaria... | lld:pubmed |
| pubmed-article:12659833 | pubmed:author | pubmed-author:YoshiharaShin... | lld:pubmed |
| pubmed-article:12659833 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12659833 | pubmed:day | 4 | lld:pubmed |
| pubmed-article:12659833 | pubmed:volume | 303 | lld:pubmed |
| pubmed-article:12659833 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12659833 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12659833 | pubmed:pagination | 419-26 | lld:pubmed |
| pubmed-article:12659833 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12659833 | pubmed:meshHeading | pubmed-meshheading:12659833... | lld:pubmed |
| pubmed-article:12659833 | pubmed:meshHeading | pubmed-meshheading:12659833... | lld:pubmed |
| pubmed-article:12659833 | pubmed:meshHeading | pubmed-meshheading:12659833... | lld:pubmed |
| pubmed-article:12659833 | pubmed:meshHeading | pubmed-meshheading:12659833... | lld:pubmed |
| pubmed-article:12659833 | pubmed:meshHeading | pubmed-meshheading:12659833... | lld:pubmed |
| pubmed-article:12659833 | pubmed:meshHeading | pubmed-meshheading:12659833... | lld:pubmed |
| pubmed-article:12659833 | pubmed:meshHeading | pubmed-meshheading:12659833... | lld:pubmed |
| pubmed-article:12659833 | pubmed:meshHeading | pubmed-meshheading:12659833... | lld:pubmed |
| pubmed-article:12659833 | pubmed:meshHeading | pubmed-meshheading:12659833... | lld:pubmed |
| pubmed-article:12659833 | pubmed:meshHeading | pubmed-meshheading:12659833... | lld:pubmed |
| pubmed-article:12659833 | pubmed:meshHeading | pubmed-meshheading:12659833... | lld:pubmed |
| pubmed-article:12659833 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12659833 | pubmed:articleTitle | Estrogenic activity of an environmental pollutant, 2-nitrofluorene, after metabolic activation by rat liver microsomes. | lld:pubmed |
| pubmed-article:12659833 | pubmed:affiliation | Graduate School of Biomedical Sciences, Programs for Pharmaceutical Sciences, Hiroshima University, Kasumi 1-2-3, Minami-ku, Hiroshima, Japan. | lld:pubmed |
| pubmed-article:12659833 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12659833 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12659833 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12659833 | lld:pubmed |
