| pubmed-article:12655101 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12655101 | lifeskim:mentions | umls-concept:C0319489 | lld:lifeskim |
| pubmed-article:12655101 | lifeskim:mentions | umls-concept:C0026649 | lld:lifeskim |
| pubmed-article:12655101 | lifeskim:mentions | umls-concept:C0014239 | lld:lifeskim |
| pubmed-article:12655101 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:12655101 | lifeskim:mentions | umls-concept:C1135842 | lld:lifeskim |
| pubmed-article:12655101 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
| pubmed-article:12655101 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:12655101 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
| pubmed-article:12655101 | lifeskim:mentions | umls-concept:C0004083 | lld:lifeskim |
| pubmed-article:12655101 | lifeskim:mentions | umls-concept:C0026022 | lld:lifeskim |
| pubmed-article:12655101 | lifeskim:mentions | umls-concept:C0013879 | lld:lifeskim |
| pubmed-article:12655101 | lifeskim:mentions | umls-concept:C1514583 | lld:lifeskim |
| pubmed-article:12655101 | pubmed:issue | Pt 4 | lld:pubmed |
| pubmed-article:12655101 | pubmed:dateCreated | 2003-3-25 | lld:pubmed |
| pubmed-article:12655101 | pubmed:abstractText | The subcellular localization of the hydrophobic TGBp3 protein of Poa semilatent virus (PSLV, genus Hordeivirus) was studied in transgenic plants using fluorescent microscopy to detect green fluorescent protein (GFP)-tagged protein and immunodetection with monoclonal antibodies (mAbs) raised against the GFP-based fusion expressed in E. coli. In Western blot analysis, mAbs efficiently recognized the wild-type and GFP-fused PSLV TGBp3 proteins expressed in transgenic Nicotiana benthamiana, but failed to detect TGBp3 in hordeivirus-infected plants. It was found that PSLV TGBp3 and GFP-TGBp3 had a tendency to form large protein complexes of an unknown nature. Fractionation studies revealed that TGBp3 represented an integral membrane protein and probably co-localized with an endoplasmic reticulum-derived domain. Microscopy of epidermal cells in transgenic plants demonstrated that GFP-TGBp3 localized to cell wall-associated punctate bodies, which often formed pairs of opposing discrete structures that co-localized with callose, indicating their association with the plasmodesmata-enriched cell wall fields. After mannitol-induced plasmolysis of the leaf epidermal cells in the transgenic plants, TGBp3 appeared within the cytoplasm and not at cell walls. Although TGBp3-induced bodies were normally static, most of them became motile after plasmolysis and displayed stochastic motion in the cytoplasm. | lld:pubmed |
| pubmed-article:12655101 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12655101 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12655101 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12655101 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12655101 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12655101 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12655101 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12655101 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12655101 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12655101 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12655101 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:12655101 | pubmed:issn | 0022-1317 | lld:pubmed |
| pubmed-article:12655101 | pubmed:author | pubmed-author:KalininaN ONO | lld:pubmed |
| pubmed-article:12655101 | pubmed:author | pubmed-author:SchiemannJJ | lld:pubmed |
| pubmed-article:12655101 | pubmed:author | pubmed-author:SolovyevA GAG | lld:pubmed |
| pubmed-article:12655101 | pubmed:author | pubmed-author:StroganovaT... | lld:pubmed |
| pubmed-article:12655101 | pubmed:author | pubmed-author:ErokhinaT NTN | lld:pubmed |
| pubmed-article:12655101 | pubmed:author | pubmed-author:ZamyatninA... | lld:pubmed |
| pubmed-article:12655101 | pubmed:author | pubmed-author:YelinaN ENE | lld:pubmed |
| pubmed-article:12655101 | pubmed:author | pubmed-author:MorozovS YuSY | lld:pubmed |
| pubmed-article:12655101 | pubmed:author | pubmed-author:GorshkovaE... | lld:pubmed |
| pubmed-article:12655101 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12655101 | pubmed:volume | 84 | lld:pubmed |
| pubmed-article:12655101 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12655101 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12655101 | pubmed:pagination | 985-94 | lld:pubmed |
| pubmed-article:12655101 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12655101 | pubmed:meshHeading | pubmed-meshheading:12655101... | lld:pubmed |
| pubmed-article:12655101 | pubmed:meshHeading | pubmed-meshheading:12655101... | lld:pubmed |
| pubmed-article:12655101 | pubmed:meshHeading | pubmed-meshheading:12655101... | lld:pubmed |
| pubmed-article:12655101 | pubmed:meshHeading | pubmed-meshheading:12655101... | lld:pubmed |
| pubmed-article:12655101 | pubmed:meshHeading | pubmed-meshheading:12655101... | lld:pubmed |
| pubmed-article:12655101 | pubmed:meshHeading | pubmed-meshheading:12655101... | lld:pubmed |
| pubmed-article:12655101 | pubmed:meshHeading | pubmed-meshheading:12655101... | lld:pubmed |
| pubmed-article:12655101 | pubmed:meshHeading | pubmed-meshheading:12655101... | lld:pubmed |
| pubmed-article:12655101 | pubmed:meshHeading | pubmed-meshheading:12655101... | lld:pubmed |
| pubmed-article:12655101 | pubmed:meshHeading | pubmed-meshheading:12655101... | lld:pubmed |
| pubmed-article:12655101 | pubmed:meshHeading | pubmed-meshheading:12655101... | lld:pubmed |
| pubmed-article:12655101 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12655101 | pubmed:articleTitle | Immunodetection and fluorescent microscopy of transgenically expressed hordeivirus TGBp3 movement protein reveals its association with endoplasmic reticulum elements in close proximity to plasmodesmata. | lld:pubmed |
| pubmed-article:12655101 | pubmed:affiliation | Department of Virology and A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russia. | lld:pubmed |
| pubmed-article:12655101 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12655101 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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