| pubmed-article:12649158 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12649158 | lifeskim:mentions | umls-concept:C0026764 | lld:lifeskim |
| pubmed-article:12649158 | lifeskim:mentions | umls-concept:C0205918 | lld:lifeskim |
| pubmed-article:12649158 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:12649158 | pubmed:dateCreated | 2003-7-1 | lld:pubmed |
| pubmed-article:12649158 | pubmed:abstractText | Multiple myeloma is a disseminated neoplasm of terminally differentiated plasma cells that is incurable with currently available therapies. Although the disease is radiosensitive, external beam radiation leads to significant toxicity due to sensitive end-organ damage. Thus, genetic approaches for therapy are required. We hypothesized that the incorporation of immunoglobulin promoter and enhancer elements in a self-inactivating (SIN) lentiviral vector should lead to specific and high-level transgene expression in myeloma cells. A SIN lentivector with enhanced green fluorescent protein (EGFP) expression under the control of a minimal immunoglobulin promoter as well as the Kappa light chain intronic and 3' enhancers transduced myeloma cell lines with high efficiency (30%-90%). EGFP was expressed at a high level in myeloma cells but silent in all nonmyeloma cell lines tested compared with the cytomegalovirus (CMV) promoter/enhancer. Transduction of myeloma cells with the targeted vector coding for the human sodiumiodide symporter (hNIS) led to hNIS expression by these cells allowing them to concentrate radioiodine up to 18-fold compared with controls. Tumor xenografts in severe combined immunodeficiency mice expressing hNIS could be imaged using iodine-123 (123I) and shown to retain iodide for up to 48 hours. These tumor xenografts were completely eradicated by a single dose of the therapeutic isotope iodine-131 (131I) without evidence of recurrence up to 5 months after therapy. We conclude that lentivectors can be transcriptionally targeted for myeloma cells and the use of hNIS as a therapeutic gene for myeloma in combination with 131I needs further exploration. | lld:pubmed |
| pubmed-article:12649158 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12649158 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12649158 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12649158 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:12649158 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12649158 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12649158 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12649158 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12649158 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12649158 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12649158 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12649158 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12649158 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12649158 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12649158 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:12649158 | pubmed:issn | 0006-4971 | lld:pubmed |
| pubmed-article:12649158 | pubmed:author | pubmed-author:MorrisJohn... | lld:pubmed |
| pubmed-article:12649158 | pubmed:author | pubmed-author:RussellStephe... | lld:pubmed |
| pubmed-article:12649158 | pubmed:author | pubmed-author:DiazRosa... | lld:pubmed |
| pubmed-article:12649158 | pubmed:author | pubmed-author:O'ConnorMicha... | lld:pubmed |
| pubmed-article:12649158 | pubmed:author | pubmed-author:DingliDavidD | lld:pubmed |
| pubmed-article:12649158 | pubmed:author | pubmed-author:BergertElizab... | lld:pubmed |
| pubmed-article:12649158 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12649158 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:12649158 | pubmed:volume | 102 | lld:pubmed |
| pubmed-article:12649158 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12649158 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12649158 | pubmed:pagination | 489-96 | lld:pubmed |
| pubmed-article:12649158 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:12649158 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12649158 | pubmed:articleTitle | Genetically targeted radiotherapy for multiple myeloma. | lld:pubmed |
| pubmed-article:12649158 | pubmed:affiliation | Mayo Clinic and Foundation, Rochester, MN, USA. | lld:pubmed |
| pubmed-article:12649158 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12649158 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:12649158 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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