| pubmed-article:12625533 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12625533 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
| pubmed-article:12625533 | lifeskim:mentions | umls-concept:C0013935 | lld:lifeskim |
| pubmed-article:12625533 | lifeskim:mentions | umls-concept:C0005712 | lld:lifeskim |
| pubmed-article:12625533 | lifeskim:mentions | umls-concept:C1522384 | lld:lifeskim |
| pubmed-article:12625533 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:12625533 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:12625533 | lifeskim:mentions | umls-concept:C1834741 | lld:lifeskim |
| pubmed-article:12625533 | lifeskim:mentions | umls-concept:C0376249 | lld:lifeskim |
| pubmed-article:12625533 | lifeskim:mentions | umls-concept:C0205171 | lld:lifeskim |
| pubmed-article:12625533 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:12625533 | pubmed:dateCreated | 2003-3-10 | lld:pubmed |
| pubmed-article:12625533 | pubmed:abstractText | Assessment of nuclear status is important when a biopsied single blastomere is used for embryo sexing. In this study we investigated the nuclear status of blastomeres derived from 8- to 16-cell stage in vitro fertilised bovine embryos to determine the representativeness of a single blastomere for embryo sexing. In 24 embryos analysed, the agreement in sex determination between a biopsied single blastomere and a matched blastocyst by polymerase chain reaction (PCR) was 83.3%. To clarify the discrepancies, karyotypes of blastomeres in 8- to 16-cell stage bovine embryos were analysed. We applied vinblastine sulfate at various concentrations and for different exposure times for metaphase plate induction in 8- to 16-cell stage bovine embryos. The 1.0 mg/ml vinblastine sulfate treatment for 15 h was selected as the most effective condition for induction of a metaphase plate (> 45%). Among 22 embryos under these conditions, only 8 of 10 that had a normal diploid chromosome complement showed a sex chromosomal composition of XX or XY (36.4%) and 2 diploid embryos showed mosaicism of the opposite sex of XX and XY in blastomeres of the embryo (9.1%). One haploid embryo contained only one X-chromosome (4.5%). Four of another 11 embryos with a mixoploid chromosomal complement contained a haploid blastomere with a wrong sex chromosome (18.2%). In conclusion, assessment of nuclear status of 8- to 16-cell stage bovine embryos revealed that morphologically normal embryos had a considerable proportion of mixoploid blastomeres and sex chromosomal mosaicism; these could be the cause of discrepancies in the sex between biopsied single blastomeres and matched blastocysts by PCR. | lld:pubmed |
| pubmed-article:12625533 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12625533 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12625533 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12625533 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12625533 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12625533 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:12625533 | pubmed:issn | 0967-1994 | lld:pubmed |
| pubmed-article:12625533 | pubmed:author | pubmed-author:LeeJong HoJH | lld:pubmed |
| pubmed-article:12625533 | pubmed:author | pubmed-author:LeeSeong HoSH | lld:pubmed |
| pubmed-article:12625533 | pubmed:author | pubmed-author:ParkJoong... | lld:pubmed |
| pubmed-article:12625533 | pubmed:author | pubmed-author:ChoiEun JooEJ | lld:pubmed |
| pubmed-article:12625533 | pubmed:author | pubmed-author:YoonJong... | lld:pubmed |
| pubmed-article:12625533 | pubmed:author | pubmed-author:ParkChang... | lld:pubmed |
| pubmed-article:12625533 | pubmed:author | pubmed-author:ImKyung... | lld:pubmed |
| pubmed-article:12625533 | pubmed:author | pubmed-author:JinDong IlDI | lld:pubmed |
| pubmed-article:12625533 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12625533 | pubmed:volume | 11 | lld:pubmed |
| pubmed-article:12625533 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12625533 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12625533 | pubmed:pagination | 87-93 | lld:pubmed |
| pubmed-article:12625533 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
| pubmed-article:12625533 | pubmed:meshHeading | pubmed-meshheading:12625533... | lld:pubmed |
| pubmed-article:12625533 | pubmed:meshHeading | pubmed-meshheading:12625533... | lld:pubmed |
| pubmed-article:12625533 | pubmed:meshHeading | pubmed-meshheading:12625533... | lld:pubmed |
| pubmed-article:12625533 | pubmed:meshHeading | pubmed-meshheading:12625533... | lld:pubmed |
| pubmed-article:12625533 | pubmed:meshHeading | pubmed-meshheading:12625533... | lld:pubmed |
| pubmed-article:12625533 | pubmed:meshHeading | pubmed-meshheading:12625533... | lld:pubmed |
| pubmed-article:12625533 | pubmed:meshHeading | pubmed-meshheading:12625533... | lld:pubmed |
| pubmed-article:12625533 | pubmed:meshHeading | pubmed-meshheading:12625533... | lld:pubmed |
| pubmed-article:12625533 | pubmed:meshHeading | pubmed-meshheading:12625533... | lld:pubmed |
| pubmed-article:12625533 | pubmed:meshHeading | pubmed-meshheading:12625533... | lld:pubmed |
| pubmed-article:12625533 | pubmed:meshHeading | pubmed-meshheading:12625533... | lld:pubmed |
| pubmed-article:12625533 | pubmed:meshHeading | pubmed-meshheading:12625533... | lld:pubmed |
| pubmed-article:12625533 | pubmed:meshHeading | pubmed-meshheading:12625533... | lld:pubmed |
| pubmed-article:12625533 | pubmed:meshHeading | pubmed-meshheading:12625533... | lld:pubmed |
| pubmed-article:12625533 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12625533 | pubmed:articleTitle | Frequency of sex chromosomal mosaicism in bovine embryos and its effects on sexing using a single blastomere by PCR. | lld:pubmed |
| pubmed-article:12625533 | pubmed:affiliation | Institute of Animal Science and Technology, College of Agriculture and Life Sciences, Seoul National University, Suwon, Korea. | lld:pubmed |
| pubmed-article:12625533 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12625533 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
