| pubmed-article:12620339 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12620339 | lifeskim:mentions | umls-concept:C1018544 | lld:lifeskim |
| pubmed-article:12620339 | lifeskim:mentions | umls-concept:C0599874 | lld:lifeskim |
| pubmed-article:12620339 | lifeskim:mentions | umls-concept:C0250805 | lld:lifeskim |
| pubmed-article:12620339 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:12620339 | pubmed:dateCreated | 2003-3-6 | lld:pubmed |
| pubmed-article:12620339 | pubmed:abstractText | Flavonol synthase was classified as a 2-oxoglutarate-dependent dioxygenase converting natural (2R,3R)-dihydroflavonols, i.e. dihydrokaempferol, to the corresponding flavonols (kaempferol). Flavonol synthase from Citrus unshiu (Satsuma mandarin), expressed in Escherichia coli and purified to homogeneity, was shown to accept also (2S)-naringenin as a substrate, producing kaempferol in high yield and assigning sequential flavanone 3beta-hydroxylase and flavonol synthase activities to the enzyme. In contrast, dihydrokaempferol was identified as the predominant product from assays performed with the unnatural (2R)-naringenin as substrate. The product which was not converted any further on repeated incubations was identified by 1H NMR and CD spectroscopies as (-)-trans-dihydrokaempferol. The data demonstrate that Citrus flavonol synthase encompasses an additional non-specific activity trans-hydroxylating the flavanones (2S)-naringenin as well as the unnatural (2R)-naringenin at C-3. | lld:pubmed |
| pubmed-article:12620339 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12620339 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12620339 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12620339 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12620339 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12620339 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12620339 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12620339 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12620339 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12620339 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12620339 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12620339 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12620339 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12620339 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12620339 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12620339 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12620339 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:12620339 | pubmed:issn | 0031-9422 | lld:pubmed |
| pubmed-article:12620339 | pubmed:author | pubmed-author:WellmannFrank... | lld:pubmed |
| pubmed-article:12620339 | pubmed:author | pubmed-author:LukacinRichar... | lld:pubmed |
| pubmed-article:12620339 | pubmed:author | pubmed-author:BritschLothar... | lld:pubmed |
| pubmed-article:12620339 | pubmed:author | pubmed-author:MaternUlrichU | lld:pubmed |
| pubmed-article:12620339 | pubmed:author | pubmed-author:MartensStefan... | lld:pubmed |
| pubmed-article:12620339 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12620339 | pubmed:volume | 62 | lld:pubmed |
| pubmed-article:12620339 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12620339 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12620339 | pubmed:pagination | 287-92 | lld:pubmed |
| pubmed-article:12620339 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12620339 | pubmed:meshHeading | pubmed-meshheading:12620339... | lld:pubmed |
| pubmed-article:12620339 | pubmed:meshHeading | pubmed-meshheading:12620339... | lld:pubmed |
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| pubmed-article:12620339 | pubmed:meshHeading | pubmed-meshheading:12620339... | lld:pubmed |
| pubmed-article:12620339 | pubmed:meshHeading | pubmed-meshheading:12620339... | lld:pubmed |
| pubmed-article:12620339 | pubmed:meshHeading | pubmed-meshheading:12620339... | lld:pubmed |
| pubmed-article:12620339 | pubmed:meshHeading | pubmed-meshheading:12620339... | lld:pubmed |
| pubmed-article:12620339 | pubmed:meshHeading | pubmed-meshheading:12620339... | lld:pubmed |
| pubmed-article:12620339 | pubmed:meshHeading | pubmed-meshheading:12620339... | lld:pubmed |
| pubmed-article:12620339 | pubmed:meshHeading | pubmed-meshheading:12620339... | lld:pubmed |
| pubmed-article:12620339 | pubmed:meshHeading | pubmed-meshheading:12620339... | lld:pubmed |
| pubmed-article:12620339 | pubmed:meshHeading | pubmed-meshheading:12620339... | lld:pubmed |
| pubmed-article:12620339 | pubmed:meshHeading | pubmed-meshheading:12620339... | lld:pubmed |
| pubmed-article:12620339 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12620339 | pubmed:articleTitle | Flavonol synthase from Citrus unshiu is a bifunctional dioxygenase. | lld:pubmed |
| pubmed-article:12620339 | pubmed:affiliation | Institut für Pharmazeutische Biologie, Philipps-Universität Marburg, Deutschhausstrasse 17 A, D-35037 Marburg, Germany. | lld:pubmed |
| pubmed-article:12620339 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12620339 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12620339 | lld:pubmed |
