| pubmed-article:12615322 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12615322 | lifeskim:mentions | umls-concept:C0032148 | lld:lifeskim |
| pubmed-article:12615322 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:12615322 | lifeskim:mentions | umls-concept:C0032150 | lld:lifeskim |
| pubmed-article:12615322 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:12615322 | lifeskim:mentions | umls-concept:C1145667 | lld:lifeskim |
| pubmed-article:12615322 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:12615322 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:12615322 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:12615322 | pubmed:dateCreated | 2003-3-4 | lld:pubmed |
| pubmed-article:12615322 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12615322 | pubmed:abstractText | Plasmodium falciparum iron regulatory-like protein (PfIRPa, accession AJ012289) has homology to a family of iron-responsive element (IRE)-binding proteins (IRPs) found in different species. We have previously demonstrated that erythrocyte P. falciparum PfIRPa binds a mammalian consensus IRE and that the binding activity is regulated by iron status. In the work we now report, we have cloned a C-terminus histidine-tagged PfIRPa and overexpressed it in a bacterial expression system in soluble form capable of binding IREs. To overexpress PfIRPa, we used the T7 promoter-driven vector, pET28a(+), in conjunction with the Rosetta(DE3)pLysS strain of E. coli, which carries extra copies of tRNA genes usually found in organisms such as P. falciparum whose genome is (A+T)-rich. The histidine-tagged recombinant protein (rPfIRPa) in soluble form was partially purified using His-bind resin. We searched the plasmodial database, plasmoDB, to identify sequences capable of forming IRE loops using a specially developed algorithm, and found three plasmodial sequences matching the search criteria. In gel retardation assays, rPfIRPa bound three 32P-labeled putative plasmodial IREs with affinity exceeding the affinity for the mammalian consensus IRE. The binding was concentration-dependent and was not inhibited by heparin, an inhibitor of non-specific binding. Immunodepletion of rPfIRPa resulted in substantial inhibition of the signal intensity in the gel retardation assays and in Western blot-determinations of rPfIRPa protein levels. Endogenous PfIRPa retained all three putative 32P-IREs at the same position on the gel as the recombinant PfIRPa. | lld:pubmed |
| pubmed-article:12615322 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12615322 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12615322 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12615322 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12615322 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12615322 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12615322 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12615322 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12615322 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12615322 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12615322 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12615322 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12615322 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12615322 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12615322 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12615322 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12615322 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:12615322 | pubmed:issn | 0166-6851 | lld:pubmed |
| pubmed-article:12615322 | pubmed:author | pubmed-author:GordeukVictor... | lld:pubmed |
| pubmed-article:12615322 | pubmed:author | pubmed-author:YikilmazEmine... | lld:pubmed |
| pubmed-article:12615322 | pubmed:author | pubmed-author:WoottonJohn... | lld:pubmed |
| pubmed-article:12615322 | pubmed:author | pubmed-author:RouaultTracey... | lld:pubmed |
| pubmed-article:12615322 | pubmed:author | pubmed-author:AltschulSteph... | lld:pubmed |
| pubmed-article:12615322 | pubmed:author | pubmed-author:LoyevskyMarkM | lld:pubmed |
| pubmed-article:12615322 | pubmed:author | pubmed-author:MompointFarah... | lld:pubmed |
| pubmed-article:12615322 | pubmed:author | pubmed-author:MaddenThomasT | lld:pubmed |
| pubmed-article:12615322 | pubmed:author | pubmed-author:Kurantsin-Mil... | lld:pubmed |
| pubmed-article:12615322 | pubmed:author | pubmed-author:KassimOlakunl... | lld:pubmed |
| pubmed-article:12615322 | pubmed:copyrightInfo | Copyright 2002 Elsevier Science B.V. | lld:pubmed |
| pubmed-article:12615322 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12615322 | pubmed:volume | 126 | lld:pubmed |
| pubmed-article:12615322 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12615322 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12615322 | pubmed:pagination | 231-8 | lld:pubmed |
| pubmed-article:12615322 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:12615322 | pubmed:meshHeading | pubmed-meshheading:12615322... | lld:pubmed |
| pubmed-article:12615322 | pubmed:meshHeading | pubmed-meshheading:12615322... | lld:pubmed |
| pubmed-article:12615322 | pubmed:meshHeading | pubmed-meshheading:12615322... | lld:pubmed |
| pubmed-article:12615322 | pubmed:meshHeading | pubmed-meshheading:12615322... | lld:pubmed |
| pubmed-article:12615322 | pubmed:meshHeading | pubmed-meshheading:12615322... | lld:pubmed |
| pubmed-article:12615322 | pubmed:meshHeading | pubmed-meshheading:12615322... | lld:pubmed |
| pubmed-article:12615322 | pubmed:meshHeading | pubmed-meshheading:12615322... | lld:pubmed |
| pubmed-article:12615322 | pubmed:meshHeading | pubmed-meshheading:12615322... | lld:pubmed |
| pubmed-article:12615322 | pubmed:meshHeading | pubmed-meshheading:12615322... | lld:pubmed |
| pubmed-article:12615322 | pubmed:meshHeading | pubmed-meshheading:12615322... | lld:pubmed |
| pubmed-article:12615322 | pubmed:meshHeading | pubmed-meshheading:12615322... | lld:pubmed |
| pubmed-article:12615322 | pubmed:meshHeading | pubmed-meshheading:12615322... | lld:pubmed |
| pubmed-article:12615322 | pubmed:meshHeading | pubmed-meshheading:12615322... | lld:pubmed |
| pubmed-article:12615322 | pubmed:meshHeading | pubmed-meshheading:12615322... | lld:pubmed |
| pubmed-article:12615322 | pubmed:meshHeading | pubmed-meshheading:12615322... | lld:pubmed |
| pubmed-article:12615322 | pubmed:meshHeading | pubmed-meshheading:12615322... | lld:pubmed |
| pubmed-article:12615322 | pubmed:meshHeading | pubmed-meshheading:12615322... | lld:pubmed |
| pubmed-article:12615322 | pubmed:meshHeading | pubmed-meshheading:12615322... | lld:pubmed |
| pubmed-article:12615322 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12615322 | pubmed:articleTitle | Expression of a recombinant IRP-like Plasmodium falciparum protein that specifically binds putative plasmodial IREs. | lld:pubmed |
| pubmed-article:12615322 | pubmed:affiliation | Center for Sickle Cell Disease, Howard University, 2121 Georgia Avenue, NW, Washington, DC 20059, USA. mloyevsky@howard.edu | lld:pubmed |
| pubmed-article:12615322 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12615322 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12615322 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12615322 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12615322 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12615322 | lld:pubmed |
