| pubmed-article:12612069 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12612069 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:12612069 | lifeskim:mentions | umls-concept:C0005456 | lld:lifeskim |
| pubmed-article:12612069 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:12612069 | lifeskim:mentions | umls-concept:C0851827 | lld:lifeskim |
| pubmed-article:12612069 | lifeskim:mentions | umls-concept:C1701901 | lld:lifeskim |
| pubmed-article:12612069 | lifeskim:mentions | umls-concept:C0971887 | lld:lifeskim |
| pubmed-article:12612069 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:12612069 | pubmed:dateCreated | 2003-3-3 | lld:pubmed |
| pubmed-article:12612069 | pubmed:abstractText | Methylation of cytosine in CpG dinucleotides promotes transcriptional repression in mammals by blocking transcription factor binding and recruiting methyl-binding proteins that initiate chromatin remodeling. Here, we use a novel cell-based system to show that retrovirally expressed Pax-5 protein activates endogenous early B-cell-specific mb-1 genes in plasmacytoma cells, but only when the promoter is hypomethylated. CpG methylation does not directly affect binding of the promoter by Pax-5. Instead, methylation of an adjacent CpG interferes with assembly of ternary complexes comprising Pax-5 and Ets proteins. In electrophoretic mobility shift assays, recruitment of Ets-1 is blocked by methylation of the Ets site (5'CCGGAG) on the antisense strand. In transfection assays, selective methylation of a single CpG within the Pax-5-dependent Ets site greatly reduces mb-1 promoter activity. Prior demethylation of the endogenous mb-1 promoter is required for its activation by Pax-5 in transduced cells. Although B-lineage cells have only unmethylated mb-1 genes and do not modulate methylation of the mb-1 promoter during development, other tissues feature high percentages of methylated alleles. Together, these studies demonstrate a novel DNA methylation-dependent mechanism for regulating transcriptional activity through the inhibition of DNA-dependent protein-protein interactions. | lld:pubmed |
| pubmed-article:12612069 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12612069 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12612069 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12612069 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12612069 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:12612069 | pubmed:issn | 0270-7306 | lld:pubmed |
| pubmed-article:12612069 | pubmed:author | pubmed-author:ClarkDawn RDR | lld:pubmed |
| pubmed-article:12612069 | pubmed:author | pubmed-author:FitzsimmonsDa... | lld:pubmed |
| pubmed-article:12612069 | pubmed:author | pubmed-author:HagmanJamesJ | lld:pubmed |
| pubmed-article:12612069 | pubmed:author | pubmed-author:MaierHollyH | lld:pubmed |
| pubmed-article:12612069 | pubmed:author | pubmed-author:ColbertJeffJ | lld:pubmed |
| pubmed-article:12612069 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12612069 | pubmed:volume | 23 | lld:pubmed |
| pubmed-article:12612069 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12612069 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12612069 | pubmed:pagination | 1946-60 | lld:pubmed |
| pubmed-article:12612069 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:12612069 | pubmed:meshHeading | pubmed-meshheading:12612069... | lld:pubmed |
| pubmed-article:12612069 | pubmed:meshHeading | pubmed-meshheading:12612069... | lld:pubmed |
| pubmed-article:12612069 | pubmed:meshHeading | pubmed-meshheading:12612069... | lld:pubmed |
| pubmed-article:12612069 | pubmed:meshHeading | pubmed-meshheading:12612069... | lld:pubmed |
| pubmed-article:12612069 | pubmed:meshHeading | pubmed-meshheading:12612069... | lld:pubmed |
| pubmed-article:12612069 | pubmed:meshHeading | pubmed-meshheading:12612069... | lld:pubmed |
| pubmed-article:12612069 | pubmed:meshHeading | pubmed-meshheading:12612069... | lld:pubmed |
| pubmed-article:12612069 | pubmed:meshHeading | pubmed-meshheading:12612069... | lld:pubmed |
