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pubmed-article:12609733pubmed:abstractTextAn approach for rapid differentiation between short-chain-length (scl) and medium-chain-length (mcl) polyhydroxyalkanoate (PHA) producers was developed. Polyhydroxyalkanoate-accumulated bacterial cells stained with Nile red were suspended in water and subjected to fluorescence spectroscopy at a fixed excitation wavelength of 488 nm. The scl-PHA-accumulated bacteria revealed a maximum emission wavelength at 590 nm, and for mcl-PHA producers were seen at a wavelength of 575 nm. Combining Nile red staining and fluorescence spectroscopy, the accumulated PHA granules could be rapidly differentiated into scl-PHA and mcl-PHA from the intact cells.lld:pubmed
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pubmed-article:12609733pubmed:authorpubmed-author:LeeChia-YinCYlld:pubmed
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pubmed-article:12609733pubmed:pagination131-5lld:pubmed
pubmed-article:12609733pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:12609733pubmed:articleTitleRapid differentiation between short-chain-length and medium-chain-length polyhydroxyalkanoate-accumulating bacteria with spectrofluorometry.lld:pubmed
pubmed-article:12609733pubmed:affiliationDepartment of Agricultural Chemistry, National Taiwan University, 1, Sec. 4, Roosevelt Rd., Taipei 106, Taiwan.lld:pubmed
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