| pubmed-article:12603212 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12603212 | lifeskim:mentions | umls-concept:C1709854 | lld:lifeskim |
| pubmed-article:12603212 | lifeskim:mentions | umls-concept:C0229671 | lld:lifeskim |
| pubmed-article:12603212 | lifeskim:mentions | umls-concept:C0225336 | lld:lifeskim |
| pubmed-article:12603212 | lifeskim:mentions | umls-concept:C0022646 | lld:lifeskim |
| pubmed-article:12603212 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
| pubmed-article:12603212 | lifeskim:mentions | umls-concept:C0022144 | lld:lifeskim |
| pubmed-article:12603212 | lifeskim:mentions | umls-concept:C1444748 | lld:lifeskim |
| pubmed-article:12603212 | lifeskim:mentions | umls-concept:C0205332 | lld:lifeskim |
| pubmed-article:12603212 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:12603212 | pubmed:dateCreated | 2003-2-26 | lld:pubmed |
| pubmed-article:12603212 | pubmed:abstractText | The vascular endothelium of transplanted organs represents an important target for allograft-directed immune responses. Although HLA antigens expressed on graft endothelial cells (EC) can become targets of the host immune response, the role of other, non-HLA-encoded EC antigens has been proposed but is still unclear. The aim of this study was to investigate the presence of and to characterize anti-EC antibodies (AECA) in 57 kidney transplant recipients according to their HLA-immunization status. Flow cytometry in pretransplant sera was used to detect AECA reactive with surface antigens on ABO and HLA-typed primary cultures of arterial ECs, stimulated or not with tumor necrosis factor-alpha (TNFalpha) or interferon-gamma (IFNgamma). FACS analysis revealed the presence of AECA in 47% of HLA-sensitized (PRA = 10%: mostly IgG) vs. 16.0% in nonsensitized patients (PRA < 10%) (p < 0.02). No significant correlation was found between the presence of AECA and acute rejection occurrence and graft outcome. Non-HLA reactive AECA are directed against TNFalpha- and IFNgamma-inducible membrane molecule(s), and react with two predominant antigens of approximately 35 kDa and approximately 50 kDa expressed on ECs but not on B cells. Binding of AECA decreases in vitro EC viability by 50-60% by promoting EC apoptosis, as demonstrated by DNA fragmentation assays. | lld:pubmed |
| pubmed-article:12603212 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12603212 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12603212 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12603212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12603212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12603212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12603212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12603212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12603212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12603212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12603212 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12603212 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:12603212 | pubmed:issn | 1600-6135 | lld:pubmed |
| pubmed-article:12603212 | pubmed:author | pubmed-author:SoulillouJean... | lld:pubmed |
| pubmed-article:12603212 | pubmed:author | pubmed-author:HourmantMaryv... | lld:pubmed |
| pubmed-article:12603212 | pubmed:author | pubmed-author:CharreauBéatr... | lld:pubmed |
| pubmed-article:12603212 | pubmed:author | pubmed-author:CoupelStéphan... | lld:pubmed |
| pubmed-article:12603212 | pubmed:author | pubmed-author:BignonJean-De... | lld:pubmed |
| pubmed-article:12603212 | pubmed:author | pubmed-author:Le... | lld:pubmed |
| pubmed-article:12603212 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12603212 | pubmed:volume | 3 | lld:pubmed |
| pubmed-article:12603212 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12603212 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12603212 | pubmed:pagination | 167-77 | lld:pubmed |
| pubmed-article:12603212 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:12603212 | pubmed:meshHeading | pubmed-meshheading:12603212... | lld:pubmed |
| pubmed-article:12603212 | pubmed:meshHeading | pubmed-meshheading:12603212... | lld:pubmed |
| pubmed-article:12603212 | pubmed:meshHeading | pubmed-meshheading:12603212... | lld:pubmed |
| pubmed-article:12603212 | pubmed:meshHeading | pubmed-meshheading:12603212... | lld:pubmed |
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| pubmed-article:12603212 | pubmed:meshHeading | pubmed-meshheading:12603212... | lld:pubmed |
| pubmed-article:12603212 | pubmed:meshHeading | pubmed-meshheading:12603212... | lld:pubmed |
| pubmed-article:12603212 | pubmed:meshHeading | pubmed-meshheading:12603212... | lld:pubmed |
| pubmed-article:12603212 | pubmed:meshHeading | pubmed-meshheading:12603212... | lld:pubmed |
| pubmed-article:12603212 | pubmed:meshHeading | pubmed-meshheading:12603212... | lld:pubmed |
| pubmed-article:12603212 | pubmed:meshHeading | pubmed-meshheading:12603212... | lld:pubmed |
| pubmed-article:12603212 | pubmed:meshHeading | pubmed-meshheading:12603212... | lld:pubmed |
| pubmed-article:12603212 | pubmed:meshHeading | pubmed-meshheading:12603212... | lld:pubmed |
| pubmed-article:12603212 | pubmed:meshHeading | pubmed-meshheading:12603212... | lld:pubmed |
| pubmed-article:12603212 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12603212 | pubmed:articleTitle | Non-HLA-type endothelial cell reactive alloantibodies in pre-transplant sera of kidney recipients trigger apoptosis. | lld:pubmed |
| pubmed-article:12603212 | pubmed:affiliation | Institut National de la Santé et de la Recherche Médicale Unité 437 'Immunointervention en Allo et Xénotransplantation', Nantes, France. | lld:pubmed |
| pubmed-article:12603212 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12603212 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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