| pubmed-article:12585481 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12585481 | lifeskim:mentions | umls-concept:C1185738 | lld:lifeskim |
| pubmed-article:12585481 | lifeskim:mentions | umls-concept:C0020291 | lld:lifeskim |
| pubmed-article:12585481 | lifeskim:mentions | umls-concept:C0001128 | lld:lifeskim |
| pubmed-article:12585481 | lifeskim:mentions | umls-concept:C0014898 | lld:lifeskim |
| pubmed-article:12585481 | lifeskim:mentions | umls-concept:C0030827 | lld:lifeskim |
| pubmed-article:12585481 | lifeskim:mentions | umls-concept:C0037098 | lld:lifeskim |
| pubmed-article:12585481 | lifeskim:mentions | umls-concept:C0008562 | lld:lifeskim |
| pubmed-article:12585481 | lifeskim:mentions | umls-concept:C0243071 | lld:lifeskim |
| pubmed-article:12585481 | lifeskim:mentions | umls-concept:C1611588 | lld:lifeskim |
| pubmed-article:12585481 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:12585481 | pubmed:dateCreated | 2003-2-14 | lld:pubmed |
| pubmed-article:12585481 | pubmed:abstractText | A technique based on liquid chromatography has been developed to facilitate studies of enantioselectivity in penicillin G acylase (PGA)-catalyzed hydrolysis of some 2-aryloxyalkanoic acid methyl esters and isosteric analogues. PGA was covalently immobilized on an aminopropyl monolithic silica support to create an immobilized HPLC-enzyme reactor. Two sets of experimental data were drawn to calculate the enantioselectivity (E) of the kinetically controlled enantiomer-differentiating reaction, the degree of substrate conversion and the enantiomeric excess of the product. The developed enzymatic reactor was coupled through a switching valve to an achiral analytical column for separation and quantitation of the hydrolysis products. The enantiomeric excess was determined off-line on a PGA-chiral stationary phase. In this way, highly precise E values were determined. A computational study related to the hydrolysis of the considered racemic esters was also carried out in order to unambiguously clarify both the substrate specificity and the enantioselectivity displayed by PGA. | lld:pubmed |
| pubmed-article:12585481 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12585481 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12585481 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12585481 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12585481 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12585481 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12585481 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12585481 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:12585481 | pubmed:issn | 0003-2700 | lld:pubmed |
| pubmed-article:12585481 | pubmed:author | pubmed-author:TortorellaPao... | lld:pubmed |
| pubmed-article:12585481 | pubmed:author | pubmed-author:MassoliniGabr... | lld:pubmed |
| pubmed-article:12585481 | pubmed:author | pubmed-author:CaccialanzaGa... | lld:pubmed |
| pubmed-article:12585481 | pubmed:author | pubmed-author:NovellinoEtto... | lld:pubmed |
| pubmed-article:12585481 | pubmed:author | pubmed-author:LubdaDieterD | lld:pubmed |
| pubmed-article:12585481 | pubmed:author | pubmed-author:FracchiollaGi... | lld:pubmed |
| pubmed-article:12585481 | pubmed:author | pubmed-author:LoiodiceFulvi... | lld:pubmed |
| pubmed-article:12585481 | pubmed:author | pubmed-author:LavecchiaAnto... | lld:pubmed |
| pubmed-article:12585481 | pubmed:author | pubmed-author:CalleriEnrica... | lld:pubmed |
| pubmed-article:12585481 | pubmed:author | pubmed-author:TemporiniCate... | lld:pubmed |
| pubmed-article:12585481 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12585481 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:12585481 | pubmed:volume | 75 | lld:pubmed |
| pubmed-article:12585481 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12585481 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12585481 | pubmed:pagination | 535-42 | lld:pubmed |
| pubmed-article:12585481 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12585481 | pubmed:meshHeading | pubmed-meshheading:12585481... | lld:pubmed |
| pubmed-article:12585481 | pubmed:meshHeading | pubmed-meshheading:12585481... | lld:pubmed |
| pubmed-article:12585481 | pubmed:meshHeading | pubmed-meshheading:12585481... | lld:pubmed |
| pubmed-article:12585481 | pubmed:meshHeading | pubmed-meshheading:12585481... | lld:pubmed |
| pubmed-article:12585481 | pubmed:meshHeading | pubmed-meshheading:12585481... | lld:pubmed |
| pubmed-article:12585481 | pubmed:meshHeading | pubmed-meshheading:12585481... | lld:pubmed |
| pubmed-article:12585481 | pubmed:meshHeading | pubmed-meshheading:12585481... | lld:pubmed |
| pubmed-article:12585481 | pubmed:meshHeading | pubmed-meshheading:12585481... | lld:pubmed |
| pubmed-article:12585481 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12585481 | pubmed:articleTitle | Enantioselective hydrolysis of some 2-aryloxyalkanoic acid methyl esters and isosteric analogues using a penicillin G acylase-based HPLC monolithic silica column. | lld:pubmed |
| pubmed-article:12585481 | pubmed:affiliation | Dipartimento di Chimica Farmaceutica, Universita' di Pavia, Via Taramani 12, 1-27100 Pavia, Italy. g.massolini@unipv.it | lld:pubmed |
| pubmed-article:12585481 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12585481 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12585481 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12585481 | lld:pubmed |
