| pubmed-article:12576053 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12576053 | lifeskim:mentions | umls-concept:C0302908 | lld:lifeskim |
| pubmed-article:12576053 | lifeskim:mentions | umls-concept:C0043220 | lld:lifeskim |
| pubmed-article:12576053 | lifeskim:mentions | umls-concept:C0002520 | lld:lifeskim |
| pubmed-article:12576053 | lifeskim:mentions | umls-concept:C0030956 | lld:lifeskim |
| pubmed-article:12576053 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:12576053 | lifeskim:mentions | umls-concept:C0004922 | lld:lifeskim |
| pubmed-article:12576053 | lifeskim:mentions | umls-concept:C1555029 | lld:lifeskim |
| pubmed-article:12576053 | lifeskim:mentions | umls-concept:C0205390 | lld:lifeskim |
| pubmed-article:12576053 | lifeskim:mentions | umls-concept:C0034760 | lld:lifeskim |
| pubmed-article:12576053 | lifeskim:mentions | umls-concept:C0037813 | lld:lifeskim |
| pubmed-article:12576053 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:12576053 | lifeskim:mentions | umls-concept:C0303920 | lld:lifeskim |
| pubmed-article:12576053 | lifeskim:mentions | umls-concept:C0059757 | lld:lifeskim |
| pubmed-article:12576053 | lifeskim:mentions | umls-concept:C0332324 | lld:lifeskim |
| pubmed-article:12576053 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:12576053 | pubmed:dateCreated | 2003-2-10 | lld:pubmed |
| pubmed-article:12576053 | pubmed:abstractText | A new method for the sensitive determination of amino acids and peptides using the tagging reagent 2-(9-carbazole)-ethyl chloroformate (CEOC) with fluorescence (FL) detection has been developed. Identification of derivatives was carried out by liquid chromotography mass spectrometry. The chromophore in the 2-(9-fluorenyl)-ethyl chloroformate (FMOC) reagent was replaced by carbazole, which resulted in a sensitive fluorescence lerivatizing agent CEOC. CEOC can easily and quickly label peptides and amino acids. Derivatives are stable enough to be efficiently analyzed by high-performance liquid chromatography. Studies on derivatization demonstrate excellent derivative yields over the pH range 8.8-10.0. Maximal yields close to 100% are observed with three- to fourfold molar reagent excess. Derivatives exhibit strong fluorescence and allow direct injection of the reaction mixture with no significant disturbance from the major fluorescent reagent degradation by-products, such as 2-(9-carbazole)-ethanol and bis-(2-(9-carbazole)-ethyl) carbonate. In addition, the detection responses for CEOC derivatives are compared to those obtained with FMOC. The ratios AC(CEOC)/AC(FMOC) = 1.00-1.82 for fluorescence (FL) response and AC'(CEOC)/AC'(FMOC) = 1.00-1.21 for ultraviolet (UV) response are observed (here, AC and AC' are, respectively, FL and UV response). Separation of the derivatized peptides and amino acids has been optimized on a Hypersil BDS C18 column. Excellent linear responses are observed. This method was used successfully to analyze protein hydrolysates from wool and from direct-derivatized beer. | lld:pubmed |
| pubmed-article:12576053 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12576053 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12576053 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12576053 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12576053 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12576053 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12576053 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12576053 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:12576053 | pubmed:issn | 0003-2697 | lld:pubmed |
| pubmed-article:12576053 | pubmed:author | pubmed-author:ZhangLinL | lld:pubmed |
| pubmed-article:12576053 | pubmed:author | pubmed-author:YouJinmaoJ | lld:pubmed |
| pubmed-article:12576053 | pubmed:author | pubmed-author:ZhangYukuiY | lld:pubmed |
| pubmed-article:12576053 | pubmed:author | pubmed-author:ShanYichuY | lld:pubmed |
| pubmed-article:12576053 | pubmed:author | pubmed-author:ZhenLiangL | lld:pubmed |
| pubmed-article:12576053 | pubmed:copyrightInfo | Copyright 2003 Elsevier Science (USA) | lld:pubmed |
| pubmed-article:12576053 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12576053 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:12576053 | pubmed:volume | 313 | lld:pubmed |
| pubmed-article:12576053 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12576053 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12576053 | pubmed:pagination | 17-27 | lld:pubmed |
| pubmed-article:12576053 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12576053 | pubmed:meshHeading | pubmed-meshheading:12576053... | lld:pubmed |
| pubmed-article:12576053 | pubmed:meshHeading | pubmed-meshheading:12576053... | lld:pubmed |
| pubmed-article:12576053 | pubmed:meshHeading | pubmed-meshheading:12576053... | lld:pubmed |
| pubmed-article:12576053 | pubmed:meshHeading | pubmed-meshheading:12576053... | lld:pubmed |
| pubmed-article:12576053 | pubmed:meshHeading | pubmed-meshheading:12576053... | lld:pubmed |
| pubmed-article:12576053 | pubmed:meshHeading | pubmed-meshheading:12576053... | lld:pubmed |
| pubmed-article:12576053 | pubmed:meshHeading | pubmed-meshheading:12576053... | lld:pubmed |
| pubmed-article:12576053 | pubmed:meshHeading | pubmed-meshheading:12576053... | lld:pubmed |
| pubmed-article:12576053 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12576053 | pubmed:articleTitle | Determination of peptides and amino acids from wool and beer with sensitive fluorescent reagent 2-(9-carbazole)-ethyl chloroformate by reverse phase high-performance liquid chromotography and liquid chromotography mass spectrometry. | lld:pubmed |
| pubmed-article:12576053 | pubmed:affiliation | Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 161 Zhongshan Road, Dalian 116011, China. | lld:pubmed |
| pubmed-article:12576053 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12576053 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
