pubmed-article:12574392 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:12574392 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
pubmed-article:12574392 | lifeskim:mentions | umls-concept:C0033085 | lld:lifeskim |
pubmed-article:12574392 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
pubmed-article:12574392 | lifeskim:mentions | umls-concept:C0039195 | lld:lifeskim |
pubmed-article:12574392 | lifeskim:mentions | umls-concept:C0085247 | lld:lifeskim |
pubmed-article:12574392 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
pubmed-article:12574392 | lifeskim:mentions | umls-concept:C0030956 | lld:lifeskim |
pubmed-article:12574392 | lifeskim:mentions | umls-concept:C1705822 | lld:lifeskim |
pubmed-article:12574392 | lifeskim:mentions | umls-concept:C0348011 | lld:lifeskim |
pubmed-article:12574392 | lifeskim:mentions | umls-concept:C0037791 | lld:lifeskim |
pubmed-article:12574392 | lifeskim:mentions | umls-concept:C0205225 | lld:lifeskim |
pubmed-article:12574392 | lifeskim:mentions | umls-concept:C0205232 | lld:lifeskim |
pubmed-article:12574392 | pubmed:issue | 4 | lld:pubmed |
pubmed-article:12574392 | pubmed:dateCreated | 2003-2-7 | lld:pubmed |
pubmed-article:12574392 | pubmed:abstractText | TCR with known antitumor reactivity can be genetically introduced into primary human T lymphocytes and provide promising tools for immunogene therapy of tumors. We molecularly characterized two distinct TCRs specific for the same HLA-A2-restricted peptide derived from the melanocyte differentiation Ag gp100, yet exhibiting different stringencies in peptide requirements. The existence of these two distinct gp100-specific TCRs allowed us to study the preservation of peptide fine specificity of native TCRalphabeta when engineered for TCR gene transfer into human T lymphocytes. Retroviral transduction of primary human T lymphocytes with either one of the two sets of TCRalphabeta constructs enabled T lymphocytes to specifically kill and produce TNF-alpha when triggered by native gp100(pos)/HLA-A2(pos) tumor target cells as well as gp100 peptide-loaded HLA-A2(pos) tumor cells. Peptide titration studies revealed that the cytolytic efficiencies of the T lymphocyte transductants were in the same range as those of the parental CTL clones. Moreover, primary human T lymphocytes expressing either one of the two engineered gp100-specific TCRs show cytolytic activities in response to a large panel of peptide mutants that are identical with those of the parental CTL. The finding that two gp100-specific TCR, derived from two different CTL, can be functionally introduced into primary human T lymphocytes without loss of the Ag reactivity and peptide fine specificity, holds great promise for the application of TCR gene transfer in cancer immunotherapy. | lld:pubmed |
pubmed-article:12574392 | pubmed:language | eng | lld:pubmed |
pubmed-article:12574392 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12574392 | pubmed:citationSubset | AIM | lld:pubmed |
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pubmed-article:12574392 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:12574392 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:12574392 | pubmed:month | Feb | lld:pubmed |
pubmed-article:12574392 | pubmed:issn | 0022-1767 | lld:pubmed |
pubmed-article:12574392 | pubmed:author | pubmed-author:AdemaGosse... | lld:pubmed |
pubmed-article:12574392 | pubmed:author | pubmed-author:FigdorCarl... | lld:pubmed |
pubmed-article:12574392 | pubmed:author | pubmed-author:WillemsenRalp... | lld:pubmed |
pubmed-article:12574392 | pubmed:author | pubmed-author:DebetsRenoR | lld:pubmed |
pubmed-article:12574392 | pubmed:author | pubmed-author:GratamaJan-Wi... | lld:pubmed |
pubmed-article:12574392 | pubmed:author | pubmed-author:SchaftNielsN | lld:pubmed |
pubmed-article:12574392 | pubmed:author | pubmed-author:de... | lld:pubmed |
pubmed-article:12574392 | pubmed:author | pubmed-author:LankiewiczBir... | lld:pubmed |
pubmed-article:12574392 | pubmed:author | pubmed-author:EssersBram... | lld:pubmed |
pubmed-article:12574392 | pubmed:author | pubmed-author:BolhuisReinie... | lld:pubmed |
pubmed-article:12574392 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:12574392 | pubmed:day | 15 | lld:pubmed |
pubmed-article:12574392 | pubmed:volume | 170 | lld:pubmed |
pubmed-article:12574392 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:12574392 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:12574392 | pubmed:pagination | 2186-94 | lld:pubmed |
pubmed-article:12574392 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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pubmed-article:12574392 | pubmed:year | 2003 | lld:pubmed |
pubmed-article:12574392 | pubmed:articleTitle | Peptide fine specificity of anti-glycoprotein 100 CTL is preserved following transfer of engineered TCR alpha beta genes into primary human T lymphocytes. | lld:pubmed |
pubmed-article:12574392 | pubmed:affiliation | Clinical and Tumor Immunology, Department of Medical Oncology, Erasmus Medical Center-Daniel den Hoed, Groene Hilledijk 301, 3075 EA Rotterdam, The Netherlands. | lld:pubmed |
pubmed-article:12574392 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:12574392 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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