| pubmed-article:12540293 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12540293 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:12540293 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:12540293 | lifeskim:mentions | umls-concept:C0017755 | lld:lifeskim |
| pubmed-article:12540293 | lifeskim:mentions | umls-concept:C0040627 | lld:lifeskim |
| pubmed-article:12540293 | lifeskim:mentions | umls-concept:C0076925 | lld:lifeskim |
| pubmed-article:12540293 | lifeskim:mentions | umls-concept:C1417683 | lld:lifeskim |
| pubmed-article:12540293 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:12540293 | lifeskim:mentions | umls-concept:C1428751 | lld:lifeskim |
| pubmed-article:12540293 | lifeskim:mentions | umls-concept:C0179400 | lld:lifeskim |
| pubmed-article:12540293 | lifeskim:mentions | umls-concept:C1880177 | lld:lifeskim |
| pubmed-article:12540293 | lifeskim:mentions | umls-concept:C1511938 | lld:lifeskim |
| pubmed-article:12540293 | pubmed:issue | Pt 3 | lld:pubmed |
| pubmed-article:12540293 | pubmed:dateCreated | 2003-4-18 | lld:pubmed |
| pubmed-article:12540293 | pubmed:abstractText | Islet-specific glucose-6-phosphatase (G6Pase) catalytic-subunit-related protein (IGRP) is a homologue of the catalytic subunit of G6Pase, the enzyme that catalyses the final step of the gluconeogenic pathway. The analysis of IGRP-chloramphenicol acetyltransferase (CAT) fusion-gene expression through transient transfection of islet-derived beta TC-3 cells revealed that multiple promoter regions, located between -306 and -97, are required for maximal IGRP-CAT fusion-gene expression. These regions correlated with trans -acting factor-binding sites in the IGRP promoter that were identified in beta TC-3 cells in situ using the ligation-mediated PCR (LMPCR) footprinting technique. However, the LMPCR data also revealed additional trans -acting factor-binding sites located between -97 and +1 that overlap two E-box motifs, even though this region by itself conferred minimal fusion-gene expression. The data presented here show that these E-box motifs are important for IGRP promoter activity, but that their action is only manifest in the presence of distal promoter elements. Thus mutation of either E-box motif in the context of the -306 to +3 IGRP promoter region reduces fusion-gene expression. These two E-box motifs have distinct sequences and preferentially bind NeuroD/BETA2 (neurogenic differentiation/beta-cell E box transactivator 2) and upstream stimulatory factor (USF) in vitro, consistent with the binding of both factors to the IGRP promoter in situ, as determined using the chromatin-immunoprecipitation (ChIP) assay. Based on experiments using mutated IGRP promoter constructs, we propose a model to explain how the ubiquitously expressed USF could contribute to islet-specific IGRP gene expression. | lld:pubmed |
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| pubmed-article:12540293 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12540293 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12540293 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12540293 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:12540293 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12540293 | pubmed:month | May | lld:pubmed |
| pubmed-article:12540293 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:12540293 | pubmed:author | pubmed-author:SteinRolandR | lld:pubmed |
| pubmed-article:12540293 | pubmed:author | pubmed-author:SvitekChristi... | lld:pubmed |
| pubmed-article:12540293 | pubmed:author | pubmed-author:OeserJames... | lld:pubmed |
| pubmed-article:12540293 | pubmed:author | pubmed-author:O'BrienRichar... | lld:pubmed |
| pubmed-article:12540293 | pubmed:author | pubmed-author:HendersonEvaE | lld:pubmed |
| pubmed-article:12540293 | pubmed:author | pubmed-author:MartinCyrus... | lld:pubmed |
| pubmed-article:12540293 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12540293 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:12540293 | pubmed:volume | 371 | lld:pubmed |
| pubmed-article:12540293 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12540293 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12540293 | pubmed:pagination | 675-86 | lld:pubmed |
| pubmed-article:12540293 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:12540293 | pubmed:meshHeading | pubmed-meshheading:12540293... | lld:pubmed |
| pubmed-article:12540293 | pubmed:meshHeading | pubmed-meshheading:12540293... | lld:pubmed |
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| pubmed-article:12540293 | pubmed:meshHeading | pubmed-meshheading:12540293... | lld:pubmed |
| pubmed-article:12540293 | pubmed:year | 2003 | lld:pubmed |
