| pubmed-article:12533671 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12533671 | lifeskim:mentions | umls-concept:C1167117 | lld:lifeskim |
| pubmed-article:12533671 | lifeskim:mentions | umls-concept:C0205177 | lld:lifeskim |
| pubmed-article:12533671 | lifeskim:mentions | umls-concept:C1155291 | lld:lifeskim |
| pubmed-article:12533671 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:12533671 | lifeskim:mentions | umls-concept:C0077640 | lld:lifeskim |
| pubmed-article:12533671 | lifeskim:mentions | umls-concept:C0851827 | lld:lifeskim |
| pubmed-article:12533671 | lifeskim:mentions | umls-concept:C1701901 | lld:lifeskim |
| pubmed-article:12533671 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:12533671 | pubmed:dateCreated | 2003-1-20 | lld:pubmed |
| pubmed-article:12533671 | pubmed:abstractText | Adozelesin is an alkylating minor groove DNA binder that is capable of rapidly inhibiting DNA replication in treated cells through a trans-acting mechanism and preferentially arrests cells in S phase. It has been shown previously that in cells treated with adozelesin, replication protein A (RPA) activity is deficient, and the middle subunit of RPA is hyperphosphorylated. The adozelesin-induced RPA hyperphosphorylation can be blocked by the replicative DNA polymerase inhibitor, aphidicolin, suggesting that adozelesin-triggered cellular DNA damage responses require active DNA replication forks. These data imply that cellular DNA damage responses to adozelesin treatment are preferentially induced in S phase. Here, we show that RPA hyperphosphorylation, RPA intranuclear focalization, and gamma-H2AX intranuclear focalization induced by adozelesin treatment are all dependent on DNA replication fork progression, and focalization is only induced in S phase cells. These findings are similar to those seen with the S phase-specific DNA-damaging agent, camptothecin. Conversely, all three DNA damage responses are independent of either S phase or replication fork progression when induced by treatment with the DNA strand scission agent, C-1027. Furthermore, we demonstrate that adozelesin-induced RPA and gamma-H2AX intranuclear foci appear to colocalize within the nuclei of S phase cells. | lld:pubmed |
| pubmed-article:12533671 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12533671 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12533671 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12533671 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12533671 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12533671 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12533671 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12533671 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12533671 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12533671 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12533671 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12533671 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12533671 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12533671 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12533671 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12533671 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12533671 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12533671 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12533671 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:12533671 | pubmed:issn | 1535-7163 | lld:pubmed |
| pubmed-article:12533671 | pubmed:author | pubmed-author:BeermanTerry... | lld:pubmed |
| pubmed-article:12533671 | pubmed:author | pubmed-author:LiuJen-SingJS | lld:pubmed |
| pubmed-article:12533671 | pubmed:author | pubmed-author:KuoShu-RuSR | lld:pubmed |
| pubmed-article:12533671 | pubmed:author | pubmed-author:MelendyThomas... | lld:pubmed |
| pubmed-article:12533671 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12533671 | pubmed:volume | 2 | lld:pubmed |
| pubmed-article:12533671 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12533671 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12533671 | pubmed:pagination | 41-7 | lld:pubmed |
| pubmed-article:12533671 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:12533671 | pubmed:meshHeading | pubmed-meshheading:12533671... | lld:pubmed |
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| pubmed-article:12533671 | pubmed:meshHeading | pubmed-meshheading:12533671... | lld:pubmed |
| pubmed-article:12533671 | pubmed:meshHeading | pubmed-meshheading:12533671... | lld:pubmed |
| pubmed-article:12533671 | pubmed:meshHeading | pubmed-meshheading:12533671... | lld:pubmed |
| pubmed-article:12533671 | pubmed:meshHeading | pubmed-meshheading:12533671... | lld:pubmed |
| pubmed-article:12533671 | pubmed:meshHeading | pubmed-meshheading:12533671... | lld:pubmed |
| pubmed-article:12533671 | pubmed:meshHeading | pubmed-meshheading:12533671... | lld:pubmed |
| pubmed-article:12533671 | pubmed:meshHeading | pubmed-meshheading:12533671... | lld:pubmed |
| pubmed-article:12533671 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12533671 | pubmed:articleTitle | Induction of DNA damage responses by adozelesin is S phase-specific and dependent on active replication forks. | lld:pubmed |
| pubmed-article:12533671 | pubmed:affiliation | Departments of Microbiology and Biochemistry and the Witebsky Center for Microbial Pathogenesis and Immunology, University at Buffalo, SUNY, School of Medicine and Biomedical Sciences, Buffalo, New York 14214, USA. | lld:pubmed |
| pubmed-article:12533671 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12533671 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:12533671 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:12533671 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:12533671 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12533671 | lld:pubmed |
