| pubmed-article:12524423 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12524423 | lifeskim:mentions | umls-concept:C0030956 | lld:lifeskim |
| pubmed-article:12524423 | lifeskim:mentions | umls-concept:C1545588 | lld:lifeskim |
| pubmed-article:12524423 | lifeskim:mentions | umls-concept:C1514562 | lld:lifeskim |
| pubmed-article:12524423 | lifeskim:mentions | umls-concept:C1883221 | lld:lifeskim |
| pubmed-article:12524423 | lifeskim:mentions | umls-concept:C1160185 | lld:lifeskim |
| pubmed-article:12524423 | lifeskim:mentions | umls-concept:C1171350 | lld:lifeskim |
| pubmed-article:12524423 | lifeskim:mentions | umls-concept:C1883204 | lld:lifeskim |
| pubmed-article:12524423 | lifeskim:mentions | umls-concept:C0444498 | lld:lifeskim |
| pubmed-article:12524423 | lifeskim:mentions | umls-concept:C1880389 | lld:lifeskim |
| pubmed-article:12524423 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:12524423 | pubmed:dateCreated | 2003-3-10 | lld:pubmed |
| pubmed-article:12524423 | pubmed:abstractText | We have previously demonstrated that adenoviral gene transfer of the NF-kappaB inhibitor IkappaB to human islets results in protection from interleukin (IL)-1beta-mediated dysfunction and apoptosis. Here we report that human and mouse islets can be efficiently transduced by a cationic peptide transduction domain (PTD-5) without impairment of islet function. PTD mediated delivery of a peptide inhibitor of the IL-1beta-induced IkappaB kinase (IKK), derived from IKKbeta (NBD; Nemo-binding domain), and completely blocked the detrimental effects of IL-1beta on islet function and NF-kappaB activity, in a similar manner to Ad-IkappaB. We also demonstrate that mouse islets can be transduced in situ by infusion of the transduction peptide through the bile duct prior to isolation, resulting in 40% peptide transduction of the beta-cells. Delivery of the IKK inhibitor transduction fusion peptide (PTD-5-NBD) in situ to mouse islets resulted in improved islet function and viability after isolation. These results demonstrate the feasibility of using PTD-mediated delivery to transiently modify islets in situ to improve their viability and function during isolation, prior to transplantation. | lld:pubmed |
| pubmed-article:12524423 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12524423 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12524423 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12524423 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12524423 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12524423 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12524423 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12524423 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12524423 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12524423 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12524423 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12524423 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12524423 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12524423 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12524423 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12524423 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12524423 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12524423 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12524423 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12524423 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:12524423 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:12524423 | pubmed:author | pubmed-author:BalamuruganA... | lld:pubmed |
| pubmed-article:12524423 | pubmed:author | pubmed-author:TruccoMassimo... | lld:pubmed |
| pubmed-article:12524423 | pubmed:author | pubmed-author:BerteraSuzann... | lld:pubmed |
| pubmed-article:12524423 | pubmed:author | pubmed-author:RobbinsPaul... | lld:pubmed |
| pubmed-article:12524423 | pubmed:author | pubmed-author:MaiJeffrey... | lld:pubmed |
| pubmed-article:12524423 | pubmed:author | pubmed-author:BottinoRitaR | lld:pubmed |
| pubmed-article:12524423 | pubmed:author | pubmed-author:MiZhibaoZ | lld:pubmed |
| pubmed-article:12524423 | pubmed:author | pubmed-author:RehmanKhaja... | lld:pubmed |
| pubmed-article:12524423 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12524423 | pubmed:day | 14 | lld:pubmed |
| pubmed-article:12524423 | pubmed:volume | 278 | lld:pubmed |
| pubmed-article:12524423 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12524423 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12524423 | pubmed:pagination | 9862-8 | lld:pubmed |
| pubmed-article:12524423 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:12524423 | pubmed:meshHeading | pubmed-meshheading:12524423... | lld:pubmed |
| pubmed-article:12524423 | pubmed:meshHeading | pubmed-meshheading:12524423... | lld:pubmed |
| pubmed-article:12524423 | pubmed:meshHeading | pubmed-meshheading:12524423... | lld:pubmed |
| pubmed-article:12524423 | pubmed:meshHeading | pubmed-meshheading:12524423... | lld:pubmed |
| pubmed-article:12524423 | pubmed:meshHeading | pubmed-meshheading:12524423... | lld:pubmed |
| pubmed-article:12524423 | pubmed:meshHeading | pubmed-meshheading:12524423... | lld:pubmed |
| pubmed-article:12524423 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12524423 | pubmed:articleTitle | Protection of islets by in situ peptide-mediated transduction of the Ikappa B kinase inhibitor Nemo-binding domain peptide. | lld:pubmed |
| pubmed-article:12524423 | pubmed:affiliation | Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA. | lld:pubmed |
| pubmed-article:12524423 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12524423 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:12524423 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12524423 | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12524423 | lld:pubmed |
