| pubmed-article:12509487 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12509487 | lifeskim:mentions | umls-concept:C0030281 | lld:lifeskim |
| pubmed-article:12509487 | lifeskim:mentions | umls-concept:C0015283 | lld:lifeskim |
| pubmed-article:12509487 | lifeskim:mentions | umls-concept:C1414685 | lld:lifeskim |
| pubmed-article:12509487 | lifeskim:mentions | umls-concept:C0030685 | lld:lifeskim |
| pubmed-article:12509487 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:12509487 | lifeskim:mentions | umls-concept:C0680255 | lld:lifeskim |
| pubmed-article:12509487 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:12509487 | lifeskim:mentions | umls-concept:C0391871 | lld:lifeskim |
| pubmed-article:12509487 | lifeskim:mentions | umls-concept:C1283071 | lld:lifeskim |
| pubmed-article:12509487 | lifeskim:mentions | umls-concept:C1963578 | lld:lifeskim |
| pubmed-article:12509487 | lifeskim:mentions | umls-concept:C1521871 | lld:lifeskim |
| pubmed-article:12509487 | pubmed:issue | Pt 1 | lld:pubmed |
| pubmed-article:12509487 | pubmed:dateCreated | 2003-1-1 | lld:pubmed |
| pubmed-article:12509487 | pubmed:abstractText | Functional coupling between Ca(2+)-induced Ca(2+) release (CICR) and quantal exocytosis in 5-hydroxytryptamine-loaded INS-1 beta cells was assessed through the use of carbon fibre amperometry in combination with Fura-2. CICR was evoked by the glucagon-like-peptide-1 (GLP-1) receptor agonist exendin-4 (Ex-4) and was accompanied by quantal secretory events appearing as amperometric current spikes time-locked to the increase of [Ca(2+)](i). The action of Ex-4 was reproduced by treatment with caffeine, and the source of Ca(2+) serving as a stimulus for exocytosis originated from ryanodine and thapsigargin-sensitive Ca(2+) stores. Two distinct patterns of exocytosis occurred within 5 s following the initiation of CICR. Non-summating exocytosis (NS-type) was defined as multiple asynchronous current spikes, and the half-height duration of each spike was 12-48 ms. Summating exocytosis (S-type) was defined as a cluster of spikes. It generated a macroscopic current, the half-height duration of which was 243-682 ms. The release charge of S-type exocytosis was 3.2-fold greater than that of NS-type when measured 2 s following the initiation of secretion. NS-type exocytosis was observed frequently under conditions in which the basal Ca(2+) concentration ([Ca(2+)](B)) was low (75-150 nM), whereas S-type exocytosis predominated under conditions in which the [Ca(2+)](B) was elevated (200-275 nM). Depolarization-induced Ca(2+) influx triggered NS-type exocytosis in most cells tested, irrespective of [Ca(2+)](B). It is concluded that CICR is a highly effective stimulus for exocytosis in INS-1 cells. The increase of [Ca(2+)](i) that accompanies CICR stimulates the asynchronous release of a small number of secretory granules under conditions of low [Ca(2+)](B). When [Ca(2+)](B) is slightly elevated, CICR targets a much larger pool of secretory granules that undergo summating exocytosis. The transition from NS-type to S-type exocytosis may represent an amplification mechanism for Ca(2+)-dependent exocytosis. | lld:pubmed |
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| pubmed-article:12509487 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12509487 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12509487 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12509487 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:12509487 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12509487 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:12509487 | pubmed:issn | 0022-3751 | lld:pubmed |
| pubmed-article:12509487 | pubmed:author | pubmed-author:HolzGeorge... | lld:pubmed |
| pubmed-article:12509487 | pubmed:author | pubmed-author:KangGuoxinG | lld:pubmed |
| pubmed-article:12509487 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12509487 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:12509487 | pubmed:volume | 546 | lld:pubmed |
| pubmed-article:12509487 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12509487 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12509487 | pubmed:pagination | 175-89 | lld:pubmed |
