| pubmed-article:12495557 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12495557 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:12495557 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
| pubmed-article:12495557 | lifeskim:mentions | umls-concept:C0031727 | lld:lifeskim |
| pubmed-article:12495557 | lifeskim:mentions | umls-concept:C0075193 | lld:lifeskim |
| pubmed-article:12495557 | lifeskim:mentions | umls-concept:C0001884 | lld:lifeskim |
| pubmed-article:12495557 | lifeskim:mentions | umls-concept:C0679823 | lld:lifeskim |
| pubmed-article:12495557 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:12495557 | lifeskim:mentions | umls-concept:C1709843 | lld:lifeskim |
| pubmed-article:12495557 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:12495557 | pubmed:dateCreated | 2002-12-23 | lld:pubmed |
| pubmed-article:12495557 | pubmed:abstractText | Staurosporine induced apoptosis of RAW 264.7 cells, a mouse macrophage-like cell line, as determined by DNA fragmentation, the increase of annexin V-stained cells, and the cleavage of poly(ADP-ribose)polymerase (PARP), a substrate of caspase. Analysis of the increase in the percentage of sub-G(1) cells revealed that the DNA fragmentation occurred in a time- and concentration-dependent manner at 0.021-2.1 microM of staurosporine. Staurosporine induced phosphorylation of p38 mitogen-activated protein kinase (MAPK) but suppressed spontaneous phosphorylation of p44/42 MAPK. The p38 MAPK inhibitor SB203580, the MAPK/extracellular signal-regulated kinase kinase inhibitor PD98059 and the phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 potentiated the staurosporine-induced PARP cleavage and DNA fragmentation. The protein kinase A (PKA) inhibitor H-89 potentiated the staurosporine-induced DNA fragmentation without potentiating the PARP cleavage. In contrast, the protein kinase C (PKC) inhibitor Ro-31-8425 suppressed the PARP cleavage and DNA fragmentation. These findings suggested that staurosporine induces apoptosis via the caspase cascade in RAW 264.7 cells. The staurosporine-induced apoptosis is positively regulated by PKC, negatively regulated by p38 MAPK, p44/42 MAPK and PI3K via the caspase cascade, and negatively regulated by PKA without regulation of caspase activation. | lld:pubmed |
| pubmed-article:12495557 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12495557 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12495557 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:12495557 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12495557 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:12495557 | pubmed:issn | 0022-3573 | lld:pubmed |
| pubmed-article:12495557 | pubmed:author | pubmed-author:OhuchiKazuoK | lld:pubmed |
| pubmed-article:12495557 | pubmed:author | pubmed-author:YamakiKouyaK | lld:pubmed |
| pubmed-article:12495557 | pubmed:author | pubmed-author:HiraizumiKenj... | lld:pubmed |
| pubmed-article:12495557 | pubmed:author | pubmed-author:ZeeOkPyoO | lld:pubmed |
| pubmed-article:12495557 | pubmed:author | pubmed-author:HongJangjaJ | lld:pubmed |
| pubmed-article:12495557 | pubmed:author | pubmed-author:AhnJong... | lld:pubmed |
| pubmed-article:12495557 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12495557 | pubmed:volume | 54 | lld:pubmed |
| pubmed-article:12495557 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12495557 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12495557 | pubmed:pagination | 1535-44 | lld:pubmed |
| pubmed-article:12495557 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:12495557 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12495557 | pubmed:articleTitle | Participation of various kinases in staurosporine induced apoptosis of RAW 264.7 cells. | lld:pubmed |
| pubmed-article:12495557 | pubmed:affiliation | Laboratory of Pathophysiological Biochemistry, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba Aramaki, Aobaku, Sendai, Miyagi 980-8578, Japan. | lld:pubmed |
| pubmed-article:12495557 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12495557 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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