Optimized clinical-scale culture conditions for ex vivo selective depletion of host-reactive donor lymphocytes: a strategy for GvHD prophylaxis in allogeneic PBSC transplantation.

Source:http://linkedlifedata.com/resource/pubmed/id/12473206

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Authors

Barrett AJ, Tran T, Carter CS, Vitetta ES, Schindler J, Ghetie V, Michálek J, Donnelly S, Read EJ, Hensen L, Mavroudis D, Solomon SR, Bahceci E

Affiliation

Stem Cell Allotransplantation Section, Hematology Branch, NHLBI, National Institutes of Health, Bethesda, MD 20892-1652, USA.

Abstract

Ex vivo selective depletion (SD) is a strategy to prevent GvHD, in which host-reactive donor lymphocytes are selectively eliminated from a PBSC allograft while conserving useful donor immune function. Prior to testing this strategy in patients, our goal was to develop a clinical-scale SD process, which involves co-culture of donor lymphocytes and irradiated recipient cells, followed by the addition of an immunotoxin (IT) directed against the alpha-chain of the IL-2 receptor (CD25), expressed on activated donor T cells.

PMID
12473206