Source:http://linkedlifedata.com/resource/pubmed/id/12462561
| Subject | Predicate | Object | Context |
|---|---|---|---|
| pubmed-article:12462561 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12462561 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:12462561 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:12462561 | lifeskim:mentions | umls-concept:C0023884 | lld:lifeskim |
| pubmed-article:12462561 | lifeskim:mentions | umls-concept:C0134835 | lld:lifeskim |
| pubmed-article:12462561 | lifeskim:mentions | umls-concept:C0007582 | lld:lifeskim |
| pubmed-article:12462561 | lifeskim:mentions | umls-concept:C0035126 | lld:lifeskim |
| pubmed-article:12462561 | lifeskim:mentions | umls-concept:C0086597 | lld:lifeskim |
| pubmed-article:12462561 | lifeskim:mentions | umls-concept:C1515655 | lld:lifeskim |
| pubmed-article:12462561 | pubmed:dateCreated | 2002-12-3 | lld:pubmed |
| pubmed-article:12462561 | pubmed:abstractText | Platelets are suggested to participate in the pathogenesis of hepatic ischemia-reperfusion (I/R) injury. This study was designed to analyze platelet-endothelial cell interactions in the postischemic mouse liver in vivo and to define the role of endothelial versus platelet P-selectin for these interactions. Platelet-endothelial cell interactions were quantitatively analyzed using intravital fluorescence microscopy after lobar hepatic I/R in C57BL/6 wild-type and P-selectin-deficient mice after infusion of ex vivo rhodamine-6G-labeled wild-type and P-selectin-deficient platelets. Reperfusion injury and apoptosis were assessed by established methods. In wild-type animals, hepatic I/R caused significantly enhanced platelet-endothelial cell interactions in terminal arterioles and postsinusoidal venules as well as platelet stagnation in sinusoids. Concomitantly, transaminase and caspase-3 activities were elevated and sinusoidal perfusion was impaired. In contrast, platelet-endothelial cell interactions were nearly absent in arterioles and venules of mice lacking endothelial P-selectin, irrespective of the presence of P-selectin on infused platelets, but still significantly elevated in sinusoids. Simultaneously, sinusoidal perfusion failure was ameliorated, and transaminase- and caspase-3 activities were significantly reduced in P-selectin-deficient mice as compared with wild-type animals. The present intravital microscopic study provides, for the first time, quantitative analyses of platelet-endothelial cell interactions in the postischemic hepatic microcirculation. Our in vivo data show that endothelial P-selectin is critical for postischemic platelet-endothelial cell interactions within hepatic presinusoidal arterioles and postsinusoidal venules. P-selectin deficiency prevents microvascular injury and apoptosis after warm hepatic I/R. | lld:pubmed |
| pubmed-article:12462561 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12462561 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12462561 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12462561 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12462561 | pubmed:author | pubmed-author:BiberthalerPe... | lld:pubmed |
| pubmed-article:12462561 | pubmed:author | pubmed-author:KrombachFritz... | lld:pubmed |
| pubmed-article:12462561 | pubmed:author | pubmed-author:MessmerKonrad... | lld:pubmed |
| pubmed-article:12462561 | pubmed:author | pubmed-author:TeupserDaniel... | lld:pubmed |
| pubmed-article:12462561 | pubmed:author | pubmed-author:KhandogaAndre... | lld:pubmed |
| pubmed-article:12462561 | pubmed:author | pubmed-author:EndersGeorgG | lld:pubmed |
| pubmed-article:12462561 | pubmed:author | pubmed-author:AxmannStefanS | lld:pubmed |
| pubmed-article:12462561 | pubmed:author | pubmed-author:HutterJoergJ | lld:pubmed |
| pubmed-article:12462561 | pubmed:author | pubmed-author:LuchtingBenja... | lld:pubmed |
| pubmed-article:12462561 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12462561 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12462561 | pubmed:pagination | 529-35 | lld:pubmed |
| pubmed-article:12462561 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:12462561 | pubmed:articleTitle | P-selectin mediates platelet-endothelial cell interactions and reperfusion injury in the mouse liver in vivo. | lld:pubmed |
| pubmed-article:12462561 | pubmed:affiliation | Institute for Surgical Research, University of Munich, Germany. | lld:pubmed |
| entrez-gene:20344 | entrezgene:pubmed | pubmed-article:12462561 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:12462561 | lld:entrezgene |