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pubmed-article:12430015pubmed:dateCreated2002-11-13lld:pubmed
pubmed-article:12430015pubmed:abstractTextThree cDNAs encoding very similar but unique isoforms of chalcone synthase (EC 2.3.1.74) were isolated from a cDNA library prepared from RNA from root tissue of the Thai medicinal plant Cassia alata L. (ringworm bush, Leguminosae). Gene transcript for these three type-III polyketide synthases was found to accumulate predominantly in roots. The heterologously expressed enzymes accepted acetyl-, n-butyryl-, isovaleryl-, n-hexanoyl-, benzoyl-, cinnamoyl-, and p-coumaroyl-CoA as starter molecules and together with the co-substrate malonyl-CoA, formed multiple products. With the exception of the assay in which acetyl-CoA was used as the starter molecule, all substrates yielded a phloroglucinol derivative resulting from three sequential condensations of acetate units derived from three malonyl-CoA decarboxylations. Every substrate tested also produced two pyrone derivatives, one resulting from two acetate unit condensations (a bis-noryangonin-type pyrone derailment product) and one resulting from three acetate unit condensations (a 4-coumaroyltriacetic acid lactone-type pyrone derailment). C. alata accumulates the flavonoids quercetin, naringenin and kaempferol in roots, suggesting that the in planta function of these enzymes is the biosynthesis of root flavonoids.lld:pubmed
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pubmed-article:12430015pubmed:statusMEDLINElld:pubmed
pubmed-article:12430015pubmed:authorpubmed-author:KutchanToni...lld:pubmed
pubmed-article:12430015pubmed:authorpubmed-author:SchmidtJürgen...lld:pubmed
pubmed-article:12430015pubmed:authorpubmed-author:SamappitoSupa...lld:pubmed
pubmed-article:12430015pubmed:authorpubmed-author:PageJonathanJlld:pubmed
pubmed-article:12430015pubmed:authorpubmed-author:De-EknamkulWa...lld:pubmed
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pubmed-article:12430015pubmed:pagination64-71lld:pubmed
pubmed-article:12430015pubmed:dateRevised2006-11-15lld:pubmed
pubmed-article:12430015pubmed:articleTitleMolecular characterization of root-specific chalcone synthases from Cassia alata.lld:pubmed
pubmed-article:12430015pubmed:affiliationLeibniz-Institut für Pflanzenbiochemie, Halle/Saale, Germany.lld:pubmed
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