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| pubmed-article:12429912 | lifeskim:mentions | umls-concept:C0024660 | lld:lifeskim |
| pubmed-article:12429912 | lifeskim:mentions | umls-concept:C0069141 | lld:lifeskim |
| pubmed-article:12429912 | lifeskim:mentions | umls-concept:C0111429 | lld:lifeskim |
| pubmed-article:12429912 | lifeskim:mentions | umls-concept:C1334894 | lld:lifeskim |
| pubmed-article:12429912 | lifeskim:mentions | umls-concept:C1704851 | lld:lifeskim |
| pubmed-article:12429912 | lifeskim:mentions | umls-concept:C1332733 | lld:lifeskim |
| pubmed-article:12429912 | lifeskim:mentions | umls-concept:C1155767 | lld:lifeskim |
| pubmed-article:12429912 | lifeskim:mentions | umls-concept:C1658773 | lld:lifeskim |
| pubmed-article:12429912 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:12429912 | lifeskim:mentions | umls-concept:C1314939 | lld:lifeskim |
| pubmed-article:12429912 | lifeskim:mentions | umls-concept:C0332120 | lld:lifeskim |
| pubmed-article:12429912 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:12429912 | pubmed:dateCreated | 2002-11-13 | lld:pubmed |
| pubmed-article:12429912 | pubmed:abstractText | Centrosome duplication in mammalian cells is a highly regulated process, occurs in coordination of other cell cycle events. However, molecular exploration of this important cellular process had been difficult due to unavailability of a simple assay system. Here, using centrosomes loosely associated with nuclei isolated from cultured cells, we developed a cell-free centriole (duplication unit of the centrosome) duplication system: unduplicated centrosomes bound to the nuclei are able to undergo duplication in the presence of G1/S extracts. We show that the ability of G1/S extracts to induce centriole duplication in vitro depends on the presence of active CDK2/cyclin E. It has been shown that dissociation of centro-somal nucleophosmin (NPM)/B23 triggered by CDK2/cyclin E-mediated phosphorylation is required for initiation of centrosome duplication. We show that centriole duplication is blocked when nuclei were preincubated with the anti-NPM/B23 antibody that prevents phosphorylation of NPM/B23 by CDK2/cyclin E. These studies provide not only direct evidence for the requirement of CDK2/cyclin E and phosphorylation of NPM/B23 for centrosomes to initiate duplication, but a valuable experimental system for further exploration of the molecular regulation of centrosome duplication in somatic cells of higher animals. | lld:pubmed |
| pubmed-article:12429912 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12429912 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12429912 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12429912 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12429912 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12429912 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:12429912 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12429912 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12429912 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12429912 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12429912 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:12429912 | pubmed:issn | 1538-4101 | lld:pubmed |
| pubmed-article:12429912 | pubmed:author | pubmed-author:FukasawaKenji... | lld:pubmed |
| pubmed-article:12429912 | pubmed:author | pubmed-author:OkudaMasaruM | lld:pubmed |
| pubmed-article:12429912 | pubmed:author | pubmed-author:TaraporePheru... | lld:pubmed |
| pubmed-article:12429912 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12429912 | pubmed:volume | 1 | lld:pubmed |
| pubmed-article:12429912 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12429912 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12429912 | pubmed:pagination | 75-81 | lld:pubmed |
| pubmed-article:12429912 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:12429912 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12429912 | pubmed:articleTitle | A mammalian in vitro centriole duplication system: evidence for involvement of CDK2/cyclin E and nucleophosmin/B23 in centrosome duplication. | lld:pubmed |
| pubmed-article:12429912 | pubmed:affiliation | Department of Cell Biology, University of Cincinnati College of Medicine, P.O. Box 670521, Ohio 45267-0521, USA. | lld:pubmed |
| pubmed-article:12429912 | pubmed:publicationType | Journal Article | lld:pubmed |
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