| pubmed-article:12412135 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12412135 | lifeskim:mentions | umls-concept:C0006901 | lld:lifeskim |
| pubmed-article:12412135 | lifeskim:mentions | umls-concept:C0022169 | lld:lifeskim |
| pubmed-article:12412135 | lifeskim:mentions | umls-concept:C0032167 | lld:lifeskim |
| pubmed-article:12412135 | lifeskim:mentions | umls-concept:C1449573 | lld:lifeskim |
| pubmed-article:12412135 | pubmed:issue | 20 | lld:pubmed |
| pubmed-article:12412135 | pubmed:dateCreated | 2002-11-4 | lld:pubmed |
| pubmed-article:12412135 | pubmed:abstractText | We report the demonstration of miniaturized capillary isoelectric focusing (CIEF) in plastic microfluidic devices. Conventional CIEF technique was adapted to the microfluidic devices to separate proteins and to detect protein-protein interactions. Both acidic and basic proteins with isoelectric points (pI) ranging from 5.4 to 11.0 were rapidly focused, mobilized, and detected in a 1.2 cm long channel (50 microm deep x 120 microm wide) with a total analysis time of 150 s. In a device with a focusing distance of 4.7 cm, the separation efficiency for a basic protein, lysozyme, was achieved as high as 1.5 x 10(5) plates, corresponding to 3.2 million plates per meter. We also experimentally confirmed that IEF resolution is essentially independent of focusing length when the applied voltage is kept the same and within a range that it does not cause Joule heating. Further, we demonstrated the use of miniaturized CIEF to study the interactions between two pairs of proteins, immunoglobulin G (IgG) with protein G and anti-six histidine (anti-6xHis) with 6xHis-tagged green fluorescent protein (GFP). Using this approach, protein-protein interactions can be detected for as little as 50 fmol of protein. We believe miniaturized CIEF is useful for studying protein-protein interactions when there is a difference in pI between a protein-protein complex and its constitutent proteins. | lld:pubmed |
| pubmed-article:12412135 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12412135 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12412135 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12412135 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12412135 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12412135 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12412135 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12412135 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12412135 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12412135 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12412135 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:12412135 | pubmed:issn | 0173-0835 | lld:pubmed |
| pubmed-article:12412135 | pubmed:author | pubmed-author:FanZ HughZH | lld:pubmed |
| pubmed-article:12412135 | pubmed:author | pubmed-author:RiccoAntonio... | lld:pubmed |
| pubmed-article:12412135 | pubmed:author | pubmed-author:TanWoeiW | lld:pubmed |
| pubmed-article:12412135 | pubmed:author | pubmed-author:QiuCharmaine... | lld:pubmed |
| pubmed-article:12412135 | pubmed:author | pubmed-author:GibbonsIanI | lld:pubmed |
| pubmed-article:12412135 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12412135 | pubmed:volume | 23 | lld:pubmed |
| pubmed-article:12412135 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12412135 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12412135 | pubmed:pagination | 3638-45 | lld:pubmed |
| pubmed-article:12412135 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12412135 | pubmed:meshHeading | pubmed-meshheading:12412135... | lld:pubmed |
| pubmed-article:12412135 | pubmed:meshHeading | pubmed-meshheading:12412135... | lld:pubmed |
| pubmed-article:12412135 | pubmed:meshHeading | pubmed-meshheading:12412135... | lld:pubmed |
| pubmed-article:12412135 | pubmed:meshHeading | pubmed-meshheading:12412135... | lld:pubmed |
| pubmed-article:12412135 | pubmed:meshHeading | pubmed-meshheading:12412135... | lld:pubmed |
| pubmed-article:12412135 | pubmed:meshHeading | pubmed-meshheading:12412135... | lld:pubmed |
| pubmed-article:12412135 | pubmed:meshHeading | pubmed-meshheading:12412135... | lld:pubmed |
| pubmed-article:12412135 | pubmed:meshHeading | pubmed-meshheading:12412135... | lld:pubmed |
| pubmed-article:12412135 | pubmed:meshHeading | pubmed-meshheading:12412135... | lld:pubmed |
| pubmed-article:12412135 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12412135 | pubmed:articleTitle | Miniaturized capillary isoelectric focusing in plastic microfluidic devices. | lld:pubmed |
| pubmed-article:12412135 | pubmed:affiliation | ACLARA BioSciences, 1288 Pear Avenue, Mountain View, CA 94043, USA. | lld:pubmed |
| pubmed-article:12412135 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12412135 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:12412135 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12412135 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12412135 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12412135 | lld:pubmed |
