| pubmed-article:12388259 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12388259 | lifeskim:mentions | umls-concept:C0024109 | lld:lifeskim |
| pubmed-article:12388259 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:12388259 | lifeskim:mentions | umls-concept:C0225336 | lld:lifeskim |
| pubmed-article:12388259 | lifeskim:mentions | umls-concept:C0302600 | lld:lifeskim |
| pubmed-article:12388259 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:12388259 | pubmed:dateCreated | 2002-12-17 | lld:pubmed |
| pubmed-article:12388259 | pubmed:abstractText | Angiogenesis is one of the most recent physiological functions attributed to products of cytochrome P-450 (CYP450) enymes. To test this at a molecular level in human cells, we used a cloned cDNA for the human endothelial enzyme CYP450 2C9 (CYP2C9) to study growth as well as differentiation of human microvascular endothelial cells from the lung (HMVEC-L). Using adenoviral vectors overexpressing mRNA for CYP2C9, we show that the presence of CYP2C9 doubles thymidine incorporation and stimulates proliferation of primary cultures of endothelial cells compared with Ad5-GFP (control) in 24 h. In addition, there is a significant increase of tube formation in Matrigel after infection of HMVEC-L with Ad5-2C9 than with Ad5-GFP. More interestingly, Ad5-2C9 expressing the antisense product of CYP2C9 (2C9AS) inhibited tube formation compared with both Ad5-GFP as well as the Ad5-2C9 constructs. Finally, we tested the most abundant arachidonic acid metabolite of CYP2C9, 14,15-epoxyeicosatrienoic acid, which induced angiogenesis in vivo when embedded in Matrigel plugs and implanted in adult rats. These data support an important role for CYP2C9 in promoting angiogenesis. | lld:pubmed |
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| pubmed-article:12388259 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12388259 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12388259 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12388259 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:12388259 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12388259 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12388259 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:12388259 | pubmed:issn | 0363-6135 | lld:pubmed |
| pubmed-article:12388259 | pubmed:author | pubmed-author:MedhoraMeetha... | lld:pubmed |
| pubmed-article:12388259 | pubmed:author | pubmed-author:JacobsElizabe... | lld:pubmed |
| pubmed-article:12388259 | pubmed:author | pubmed-author:HarderDavid... | lld:pubmed |
| pubmed-article:12388259 | pubmed:author | pubmed-author:FisslthalerBe... | lld:pubmed |
| pubmed-article:12388259 | pubmed:author | pubmed-author:BusseRudiR | lld:pubmed |
| pubmed-article:12388259 | pubmed:author | pubmed-author:DanielsJohnJ | lld:pubmed |
| pubmed-article:12388259 | pubmed:author | pubmed-author:MundeyKavitaK | lld:pubmed |
| pubmed-article:12388259 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12388259 | pubmed:volume | 284 | lld:pubmed |
| pubmed-article:12388259 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12388259 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12388259 | pubmed:pagination | H215-24 | lld:pubmed |
| pubmed-article:12388259 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:12388259 | pubmed:meshHeading | pubmed-meshheading:12388259... | lld:pubmed |
| pubmed-article:12388259 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12388259 | pubmed:articleTitle | Epoxygenase-driven angiogenesis in human lung microvascular endothelial cells. | lld:pubmed |
| pubmed-article:12388259 | pubmed:affiliation | Division of Pulmonary and Critical Care, Department of Medicine, Cardiovascular Center, Medical College of Wisconsin, Milwaukee 53226, USA. medhoram@mcw.edu | lld:pubmed |
| pubmed-article:12388259 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12388259 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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