| pubmed-article:12385028 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12385028 | lifeskim:mentions | umls-concept:C1167250 | lld:lifeskim |
| pubmed-article:12385028 | lifeskim:mentions | umls-concept:C0034790 | lld:lifeskim |
| pubmed-article:12385028 | lifeskim:mentions | umls-concept:C1514762 | lld:lifeskim |
| pubmed-article:12385028 | lifeskim:mentions | umls-concept:C0681840 | lld:lifeskim |
| pubmed-article:12385028 | lifeskim:mentions | umls-concept:C1511545 | lld:lifeskim |
| pubmed-article:12385028 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:12385028 | pubmed:dateCreated | 2003-1-30 | lld:pubmed |
| pubmed-article:12385028 | pubmed:abstractText | The current model suggesting that raft integrity is required for T cell activation is mostly (but not exclusively) based on the use of drugs, such as methyl-beta-cyclodextrin (M beta CD), that disorganize rafts and inhibit T cell receptor (TCR)-induced Ca2+ influx. Here we show that conditions that disrupt lipid raft integrity do not inhibit TCR triggering in Jurkat cells and normal T lymphocytes. Indeed, we found that the reported inhibition of TCR-induced Ca2+ influx by M beta CD treatment is mainly due to (a) nonspecific depletion of intracellular Ca2+ stores and (b) plasma membrane depolarization of T cells. When these side-effects are taken into account, raft disorganization does not alter TCR-dependent Ca2+ signaling. In line with these results, also TCR-induced tyrosine phosphorylation is not inhibited by dispersion of lipid rafts. By contrast, in the same conditions, Ca2+ signaling via the glycosylphosphatidylinositol (GPI)-anchored protein CD59 is totally abolished. These results indicate that, while signaling through GPI-anchored proteins requires lipid raft integrity, CD3-dependent TCR activation occurs independently of cholesterol extraction. | lld:pubmed |
| pubmed-article:12385028 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12385028 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12385028 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12385028 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12385028 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12385028 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12385028 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12385028 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12385028 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12385028 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12385028 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:12385028 | pubmed:issn | 0014-2980 | lld:pubmed |
| pubmed-article:12385028 | pubmed:author | pubmed-author:ViolaAntonell... | lld:pubmed |
| pubmed-article:12385028 | pubmed:author | pubmed-author:PozzanTullioT | lld:pubmed |
| pubmed-article:12385028 | pubmed:author | pubmed-author:GiurisatoEman... | lld:pubmed |
| pubmed-article:12385028 | pubmed:author | pubmed-author:PizzoPaolaP | lld:pubmed |
| pubmed-article:12385028 | pubmed:author | pubmed-author:TassiMaristel... | lld:pubmed |
| pubmed-article:12385028 | pubmed:author | pubmed-author:BenedettiAnge... | lld:pubmed |
| pubmed-article:12385028 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12385028 | pubmed:volume | 32 | lld:pubmed |
| pubmed-article:12385028 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12385028 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12385028 | pubmed:pagination | 3082-91 | lld:pubmed |
| pubmed-article:12385028 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12385028 | pubmed:meshHeading | pubmed-meshheading:12385028... | lld:pubmed |
| pubmed-article:12385028 | pubmed:meshHeading | pubmed-meshheading:12385028... | lld:pubmed |
| pubmed-article:12385028 | pubmed:meshHeading | pubmed-meshheading:12385028... | lld:pubmed |
| pubmed-article:12385028 | pubmed:meshHeading | pubmed-meshheading:12385028... | lld:pubmed |
| pubmed-article:12385028 | pubmed:meshHeading | pubmed-meshheading:12385028... | lld:pubmed |
| pubmed-article:12385028 | pubmed:meshHeading | pubmed-meshheading:12385028... | lld:pubmed |
| pubmed-article:12385028 | pubmed:meshHeading | pubmed-meshheading:12385028... | lld:pubmed |
| pubmed-article:12385028 | pubmed:meshHeading | pubmed-meshheading:12385028... | lld:pubmed |
| pubmed-article:12385028 | pubmed:meshHeading | pubmed-meshheading:12385028... | lld:pubmed |
| pubmed-article:12385028 | pubmed:meshHeading | pubmed-meshheading:12385028... | lld:pubmed |
| pubmed-article:12385028 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12385028 | pubmed:articleTitle | Lipid rafts and T cell receptor signaling: a critical re-evaluation. | lld:pubmed |
| pubmed-article:12385028 | pubmed:affiliation | Department of Biomedical Sciences, University of Padova, Padova, Italy. | lld:pubmed |
| pubmed-article:12385028 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12385028 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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