| pubmed-article:1238407 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1238407 | lifeskim:mentions | umls-concept:C0024264 | lld:lifeskim |
| pubmed-article:1238407 | lifeskim:mentions | umls-concept:C0031858 | lld:lifeskim |
| pubmed-article:1238407 | lifeskim:mentions | umls-concept:C0032821 | lld:lifeskim |
| pubmed-article:1238407 | lifeskim:mentions | umls-concept:C0031164 | lld:lifeskim |
| pubmed-article:1238407 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:1238407 | lifeskim:mentions | umls-concept:C0439831 | lld:lifeskim |
| pubmed-article:1238407 | lifeskim:mentions | umls-concept:C1515926 | lld:lifeskim |
| pubmed-article:1238407 | pubmed:issue | 2 PT 2 SUPPL 1 | lld:pubmed |
| pubmed-article:1238407 | pubmed:dateCreated | 1976-2-9 | lld:pubmed |
| pubmed-article:1238407 | pubmed:abstractText | The exposure of rat and human lymphoid cells to mitogenic concentrations of phytohemagglutinin resulted in an apparent decrease in cellular K+ without a significant change in cellular Na+ when the cells were washed with isotonic Hepes buffered choline chloride prior to cation determination. The apparent reduction in total cellular Na+ plus K+ concentration, however, was not accompanied by a change in cell volume. We inferred that the constant cell volume could occur only if the lost intracellular K+ was exchanged for an external cation during the washing procedure used to prepare cells for Na+ and K+ measurement. This inference was supported by the quantitative recovery of lost cellular K+ in the choline chloride washing solution and the demonstration that a comparable proportion of 86Rb+ (K+ analogue) 42K+ was lost from prelabelled cells during choline chloride washing. Use of medium 199 with Hanks salts, 150 mM NaCl, or 100 mM MgCl2 as the washing solution did not prevent K+ exchange although exchange was less in the presence of MgCl2. These findings indicate that phytohemagglutinin produces a rapid alteration in lymphocyte plasma membranes so as to allow abnormal K+ exchange. This observation is of importance because investigators who measure intracellular solutes in phytohemagglutinin-treated lymphocytes must consider the possibility of lossduring preparative washes. Also, changes in membrane permeability following phytohemagglutinin treatment may modulate mitogenesis and/or permit the transmission of chemical messages between cells. | lld:pubmed |
| pubmed-article:1238407 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1238407 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1238407 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1238407 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1238407 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1238407 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1238407 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1238407 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1238407 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1238407 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1238407 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:1238407 | pubmed:issn | 0021-9541 | lld:pubmed |
| pubmed-article:1238407 | pubmed:author | pubmed-author:LichtmanM AMA | lld:pubmed |
| pubmed-article:1238407 | pubmed:author | pubmed-author:ScanuMM | lld:pubmed |
| pubmed-article:1238407 | pubmed:author | pubmed-author:KlempererM... | lld:pubmed |
| pubmed-article:1238407 | pubmed:author | pubmed-author:GordonB RBR | lld:pubmed |
| pubmed-article:1238407 | pubmed:author | pubmed-author:HollanderM... | lld:pubmed |
| pubmed-article:1238407 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1238407 | pubmed:volume | 86 | lld:pubmed |
| pubmed-article:1238407 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1238407 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1238407 | pubmed:pagination | 327-35 | lld:pubmed |
| pubmed-article:1238407 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:1238407 | pubmed:meshHeading | pubmed-meshheading:1238407-... | lld:pubmed |
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| pubmed-article:1238407 | pubmed:meshHeading | pubmed-meshheading:1238407-... | lld:pubmed |
| pubmed-article:1238407 | pubmed:meshHeading | pubmed-meshheading:1238407-... | lld:pubmed |
| pubmed-article:1238407 | pubmed:meshHeading | pubmed-meshheading:1238407-... | lld:pubmed |
| pubmed-article:1238407 | pubmed:meshHeading | pubmed-meshheading:1238407-... | lld:pubmed |
| pubmed-article:1238407 | pubmed:meshHeading | pubmed-meshheading:1238407-... | lld:pubmed |
| pubmed-article:1238407 | pubmed:year | 1975 | lld:pubmed |
| pubmed-article:1238407 | pubmed:articleTitle | A rapid phytohemagglutinin induced alteration in lymphocyte potassium permeability. | lld:pubmed |
| pubmed-article:1238407 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1238407 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1238407 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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