| pubmed-article:12324447 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12324447 | lifeskim:mentions | umls-concept:C0085089 | lld:lifeskim |
| pubmed-article:12324447 | lifeskim:mentions | umls-concept:C0029144 | lld:lifeskim |
| pubmed-article:12324447 | lifeskim:mentions | umls-concept:C0449468 | lld:lifeskim |
| pubmed-article:12324447 | lifeskim:mentions | umls-concept:C0205234 | lld:lifeskim |
| pubmed-article:12324447 | lifeskim:mentions | umls-concept:C0037812 | lld:lifeskim |
| pubmed-article:12324447 | lifeskim:mentions | umls-concept:C0016315 | lld:lifeskim |
| pubmed-article:12324447 | lifeskim:mentions | umls-concept:C1707520 | lld:lifeskim |
| pubmed-article:12324447 | lifeskim:mentions | umls-concept:C1517004 | lld:lifeskim |
| pubmed-article:12324447 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:12324447 | pubmed:dateCreated | 2002-9-26 | lld:pubmed |
| pubmed-article:12324447 | pubmed:abstractText | Fluorescence correlation spectroscopy (FCS) can provide a wealth of information about biological and chemical systems on a broad range of time scales (<1 micros to >1 s). Numerical modeling of the FCS observation volume combined with measurements has revealed, however, that the standard assumption of a three-dimensional Gaussian FCS observation volume is not a valid approximation under many common measurement conditions. As a result, the FCS autocorrelation will contain significant, systematic artifacts that are most severe with confocal optics when using a large detector aperture and aperture-limited illumination. These optical artifacts manifest themselves in the fluorescence correlation as an apparent additional exponential component or diffusing species with significant (>30%) amplitude that can imply extraneous kinetics, shift the measured diffusion time by as much as approximately 80%, and cause the axial ratio to diverge. Artifacts can be minimized or virtually eliminated by using a small confocal detector aperture, underfilled objective back-aperture, or two-photon excitation. However, using a detector aperture that is smaller or larger than the optimal value (approximately 4.5 optical units) greatly reduces both the count rate per molecule and the signal-to-noise ratio. Thus, there is a tradeoff between optimizing signal-to-noise and reducing experimental artifacts in one-photon FCS. | lld:pubmed |
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| pubmed-article:12324447 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12324447 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12324447 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12324447 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12324447 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:12324447 | pubmed:issn | 0006-3495 | lld:pubmed |
| pubmed-article:12324447 | pubmed:author | pubmed-author:HessSamuel... | lld:pubmed |
| pubmed-article:12324447 | pubmed:author | pubmed-author:WebbWatt WWW | lld:pubmed |
| pubmed-article:12324447 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12324447 | pubmed:volume | 83 | lld:pubmed |
| pubmed-article:12324447 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12324447 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12324447 | pubmed:pagination | 2300-17 | lld:pubmed |
| pubmed-article:12324447 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:12324447 | pubmed:meshHeading | pubmed-meshheading:12324447... | lld:pubmed |
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| pubmed-article:12324447 | pubmed:meshHeading | pubmed-meshheading:12324447... | lld:pubmed |
| pubmed-article:12324447 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12324447 | pubmed:articleTitle | Focal volume optics and experimental artifacts in confocal fluorescence correlation spectroscopy. | lld:pubmed |
| pubmed-article:12324447 | pubmed:affiliation | Department of Physics, Cornell University, Ithaca, New York 14853, USA. | lld:pubmed |
| pubmed-article:12324447 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12324447 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:12324447 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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