Linked Life Data

12206828

Source:http://linkedlifedata.com/resource/pubmed/id/12206828

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:12206828rdf:typepubmed:Citationlld:pubmed
pubmed-article:12206828lifeskim:mentionsumls-concept:C0010453lld:lifeskim
pubmed-article:12206828lifeskim:mentionsumls-concept:C0034721lld:lifeskim
pubmed-article:12206828lifeskim:mentionsumls-concept:C0034693lld:lifeskim
pubmed-article:12206828lifeskim:mentionsumls-concept:C0227525lld:lifeskim
pubmed-article:12206828lifeskim:mentionsumls-concept:C0178719lld:lifeskim
pubmed-article:12206828lifeskim:mentionsumls-concept:C0242485lld:lifeskim
pubmed-article:12206828lifeskim:mentionsumls-concept:C0034917lld:lifeskim
pubmed-article:12206828lifeskim:mentionsumls-concept:C1704711lld:lifeskim
pubmed-article:12206828lifeskim:mentionsumls-concept:C0242842lld:lifeskim
pubmed-article:12206828lifeskim:mentionsumls-concept:C0242841lld:lifeskim
pubmed-article:12206828lifeskim:mentionsumls-concept:C0066736lld:lifeskim
pubmed-article:12206828pubmed:issue5lld:pubmed
pubmed-article:12206828pubmed:dateCreated2002-9-3lld:pubmed
pubmed-article:12206828pubmed:abstractTextIntracellular reduced glutathione (GSH) plays a key role in protecting cells from toxicity by maintaining intracellular redox status, conjugating with electrophilic xenobiotics and free radicals, and detoxifying reactive peroxides. Several toxic chemicals interact with GSH during their metabolism, and in many cases it would be advantageous to monitor intracellular GSH distribution during that process. We present a novel method to monitor intracellular GSH levels utilising a new laser light source, InGaN laser, for confocal microscopy and fluorescent detection of monochlorobimane (mBCl) binding to GSH. The sensitivity of the method was compared with that obtained using o-phthalaldehyde (OPT) as a fluorochrome. In the presence of a source of glutathione S-transferase (GST), mBCl was specific for GSH, forming a fluorescent conjugate that was retained in hepatocytes for at least 35 min. mBCl was able to detect the GSH depleting effects caused by progressive inhibition of GSH synthesis by increasing concentrations of buthionine sulfoximine. It effectively monitored the rapid effects of menadione and chromium VI metabolism on intracellular GSH levels in the cytosol and nuclear compartments of the cells. The combination of a specific stain, a novel laser light source and confocal microscopy provide a valuable system for mechanistic studies of intracellular GSH distribution in toxicology studies.lld:pubmed
pubmed-article:12206828pubmed:languageenglld:pubmed
pubmed-article:12206828pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12206828pubmed:citationSubsetIMlld:pubmed
pubmed-article:12206828pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12206828pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12206828pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12206828pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12206828pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12206828pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:12206828pubmed:statusMEDLINElld:pubmed
pubmed-article:12206828pubmed:monthOctlld:pubmed
pubmed-article:12206828pubmed:issn0887-2333lld:pubmed
pubmed-article:12206828pubmed:authorpubmed-author:StevensonDDlld:pubmed
pubmed-article:12206828pubmed:authorpubmed-author:GrantM HMHlld:pubmed
pubmed-article:12206828pubmed:authorpubmed-author:WokosinDDlld:pubmed
pubmed-article:12206828pubmed:authorpubmed-author:GirkinJJlld:pubmed
pubmed-article:12206828pubmed:issnTypePrintlld:pubmed
pubmed-article:12206828pubmed:volume16lld:pubmed
pubmed-article:12206828pubmed:ownerNLMlld:pubmed
pubmed-article:12206828pubmed:authorsCompleteYlld:pubmed
pubmed-article:12206828pubmed:pagination609-19lld:pubmed
pubmed-article:12206828pubmed:dateRevised2009-4-10lld:pubmed
pubmed-article:12206828pubmed:meshHeadingpubmed-meshheading:12206828...lld:pubmed
pubmed-article:12206828pubmed:meshHeadingpubmed-meshheading:12206828...lld:pubmed
pubmed-article:12206828pubmed:meshHeadingpubmed-meshheading:12206828...lld:pubmed
pubmed-article:12206828pubmed:meshHeadingpubmed-meshheading:12206828...lld:pubmed
pubmed-article:12206828pubmed:meshHeadingpubmed-meshheading:12206828...lld:pubmed
pubmed-article:12206828pubmed:meshHeadingpubmed-meshheading:12206828...lld:pubmed
pubmed-article:12206828pubmed:meshHeadingpubmed-meshheading:12206828...lld:pubmed
pubmed-article:12206828pubmed:meshHeadingpubmed-meshheading:12206828...lld:pubmed
pubmed-article:12206828pubmed:meshHeadingpubmed-meshheading:12206828...lld:pubmed
pubmed-article:12206828pubmed:meshHeadingpubmed-meshheading:12206828...lld:pubmed
pubmed-article:12206828pubmed:meshHeadingpubmed-meshheading:12206828...lld:pubmed
pubmed-article:12206828pubmed:meshHeadingpubmed-meshheading:12206828...lld:pubmed
pubmed-article:12206828pubmed:meshHeadingpubmed-meshheading:12206828...lld:pubmed
pubmed-article:12206828pubmed:meshHeadingpubmed-meshheading:12206828...lld:pubmed
pubmed-article:12206828pubmed:meshHeadingpubmed-meshheading:12206828...lld:pubmed
pubmed-article:12206828pubmed:year2002lld:pubmed
pubmed-article:12206828pubmed:articleTitleMeasurement of the intracellular distribution of reduced glutathione in cultured rat hepatocytes using monochlorobimane and confocal laser scanning microscopy.lld:pubmed
pubmed-article:12206828pubmed:affiliationBioengineering Unit, University of Strathclyde, Wolfson Centre, 106 Rottenrow, Glasgow G4 0NW, Scotland, UK.lld:pubmed
pubmed-article:12206828pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:12206828pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:12206828pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12206828lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:12206828lld:pubmed