| pubmed-article:12185196 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12185196 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:12185196 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:12185196 | lifeskim:mentions | umls-concept:C1956003 | lld:lifeskim |
| pubmed-article:12185196 | lifeskim:mentions | umls-concept:C1704675 | lld:lifeskim |
| pubmed-article:12185196 | lifeskim:mentions | umls-concept:C0069139 | lld:lifeskim |
| pubmed-article:12185196 | lifeskim:mentions | umls-concept:C1705241 | lld:lifeskim |
| pubmed-article:12185196 | lifeskim:mentions | umls-concept:C0439097 | lld:lifeskim |
| pubmed-article:12185196 | lifeskim:mentions | umls-concept:C1547348 | lld:lifeskim |
| pubmed-article:12185196 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:12185196 | pubmed:dateCreated | 2002-8-19 | lld:pubmed |
| pubmed-article:12185196 | pubmed:abstractText | We examined the expression and cytolocalization of the protein phosphatase type 1 delta (PP1delta) isoform and nucleolin in human osteoblastic MG63 and Saos-2 cells. Cellular fractionation of MG63 cells was done and protein was prepared from each fraction. Anti-nucleolin antibody interacted with the 100- and 95-kD proteins present in the whole-cell lysate. The 100-kD protein was detected in nuclear and nucleolar fractions. The 95-kD protein was detected in cytosolic and nucleoplasmic fractions. PP1delta and nucleolin were co-localized in the nucleolus in MG63 and Saos-2 cells revealed by an immunofluorescence method. PP1delta and nucleolin were also co-immunoprecipitated with anti-nucleolin and anti-PP1delta antibodies. In the actinomycin D-treated cells, the subcellular localization of PP1delta and nucleolin was changed. Expression of PP1delta was upregulated with actinomycin D treatment. The level of 100-kD protein did not change in the actinomycin D-treated cells. However, the level of the 95-kD band increased with actinomycin D treatment. These results indicate that PP1delta was associated with nucleolin in the nucleolus of MG63 and Saos-2 cells and that nucleolin is a possible candidate substrate for PP1delta. | lld:pubmed |
| pubmed-article:12185196 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12185196 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12185196 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12185196 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12185196 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12185196 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12185196 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12185196 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12185196 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12185196 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12185196 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12185196 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12185196 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:12185196 | pubmed:issn | 0022-1554 | lld:pubmed |
| pubmed-article:12185196 | pubmed:author | pubmed-author:MorimotoHiroy... | lld:pubmed |
| pubmed-article:12185196 | pubmed:author | pubmed-author:OkamuraHirohi... | lld:pubmed |
| pubmed-article:12185196 | pubmed:author | pubmed-author:HanejiTatsuji... | lld:pubmed |
| pubmed-article:12185196 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12185196 | pubmed:volume | 50 | lld:pubmed |
| pubmed-article:12185196 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12185196 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12185196 | pubmed:pagination | 1187-93 | lld:pubmed |
| pubmed-article:12185196 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:12185196 | pubmed:meshHeading | pubmed-meshheading:12185196... | lld:pubmed |
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| pubmed-article:12185196 | pubmed:meshHeading | pubmed-meshheading:12185196... | lld:pubmed |
| pubmed-article:12185196 | pubmed:meshHeading | pubmed-meshheading:12185196... | lld:pubmed |
| pubmed-article:12185196 | pubmed:meshHeading | pubmed-meshheading:12185196... | lld:pubmed |
| pubmed-article:12185196 | pubmed:meshHeading | pubmed-meshheading:12185196... | lld:pubmed |
| pubmed-article:12185196 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12185196 | pubmed:articleTitle | Interaction of protein phosphatase 1 delta with nucleolin in human osteoblastic cells. | lld:pubmed |
| pubmed-article:12185196 | pubmed:affiliation | Department of Histology and Oral Histology, School of Dentistry, The University of Tokushima, Tokushima, Japan. | lld:pubmed |
| pubmed-article:12185196 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12185196 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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