| pubmed-article:12176897 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12176897 | lifeskim:mentions | umls-concept:C0042776 | lld:lifeskim |
| pubmed-article:12176897 | lifeskim:mentions | umls-concept:C0004561 | lld:lifeskim |
| pubmed-article:12176897 | lifeskim:mentions | umls-concept:C0039195 | lld:lifeskim |
| pubmed-article:12176897 | lifeskim:mentions | umls-concept:C0003320 | lld:lifeskim |
| pubmed-article:12176897 | lifeskim:mentions | umls-concept:C0679058 | lld:lifeskim |
| pubmed-article:12176897 | lifeskim:mentions | umls-concept:C0205369 | lld:lifeskim |
| pubmed-article:12176897 | lifeskim:mentions | umls-concept:C2826170 | lld:lifeskim |
| pubmed-article:12176897 | lifeskim:mentions | umls-concept:C1547699 | lld:lifeskim |
| pubmed-article:12176897 | lifeskim:mentions | umls-concept:C1517294 | lld:lifeskim |
| pubmed-article:12176897 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:12176897 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:12176897 | pubmed:dateCreated | 2002-8-14 | lld:pubmed |
| pubmed-article:12176897 | pubmed:abstractText | Lymphoblastoid cell lines (LCLs) are human B cells latently infected and immortalized by Epstein-Barr virus (EBV). Presenting viral antigens, they efficiently induce EBV-specific T-cell responses in vitro. Analogous ways to generate T-cell cultures specific for other antigens of interest are highly desirable. Previously, we constructed a mini-EBV plasmid that consists of less than half the EBV genome, is unable to cause virus production, but still immortalizes B cells in vitro. Mini-EBV-immortalized B-cell lines (mini-LCLs) are efficiently produced by infection of B cells with viruslike particles carrying only mini-EBV DNA. Mini-EBV plasmids can be engineered to express an additional gene in immortalized B cells. Here we present a mini-EBV coding for a potent CD8(+) T-cell antigen, the matrix phosphoprotein pp65 of human cytomegalovirus (CMV). By means of this pp65 mini-EBV, pp65-expressing mini-LCLs could be readily established from healthy donors in a one-step procedure. We used these pp65 mini-LCLs to reactivate and expand effector T cells from autologous peripheral blood cells in vitro. When generated from cytomegalovirus (CMV)-seropositive donors, these effector T-cell cultures displayed strong pp65-specific HLA-restricted cytotoxicity. A large fraction of CD8(+) T cells with pp65 epitope specificity was present in such cultures, as demonstrated by direct staining with HLA/peptide tetramers. We conclude that the pp65 mini-EBV is an attractive tool for CMV-specific adoptive immunotherapy. Mini-EBVs could also facilitate the generation of T cells specific for various other antigens of interest. | lld:pubmed |
| pubmed-article:12176897 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12176897 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12176897 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:12176897 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12176897 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12176897 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12176897 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:12176897 | pubmed:issn | 0006-4971 | lld:pubmed |
| pubmed-article:12176897 | pubmed:author | pubmed-author:MossPaul A... | lld:pubmed |
| pubmed-article:12176897 | pubmed:author | pubmed-author:CobboldMarkM | lld:pubmed |
| pubmed-article:12176897 | pubmed:author | pubmed-author:RickinsonAlan... | lld:pubmed |
| pubmed-article:12176897 | pubmed:author | pubmed-author:HislopAndrew... | lld:pubmed |
| pubmed-article:12176897 | pubmed:author | pubmed-author:KhanNaeemN | lld:pubmed |
| pubmed-article:12176897 | pubmed:author | pubmed-author:MoosmannAndre... | lld:pubmed |
| pubmed-article:12176897 | pubmed:author | pubmed-author:ZentzCaroline... | lld:pubmed |
| pubmed-article:12176897 | pubmed:author | pubmed-author:DelecluseHenr... | lld:pubmed |
| pubmed-article:12176897 | pubmed:author | pubmed-author:HollweckGabiG | lld:pubmed |
| pubmed-article:12176897 | pubmed:author | pubmed-author:BlakeNeil WNW | lld:pubmed |
| pubmed-article:12176897 | pubmed:author | pubmed-author:Croom-CarterD... | lld:pubmed |
| pubmed-article:12176897 | pubmed:author | pubmed-author:WollenbergBar... | lld:pubmed |
| pubmed-article:12176897 | pubmed:author | pubmed-author:ZeidlerReinha... | lld:pubmed |
| pubmed-article:12176897 | pubmed:author | pubmed-author:Hammerschmidt... | lld:pubmed |
| pubmed-article:12176897 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12176897 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:12176897 | pubmed:volume | 100 | lld:pubmed |
| pubmed-article:12176897 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12176897 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12176897 | pubmed:pagination | 1755-64 | lld:pubmed |
| pubmed-article:12176897 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:12176897 | pubmed:meshHeading | pubmed-meshheading:12176897... | lld:pubmed |
| pubmed-article:12176897 | pubmed:meshHeading | pubmed-meshheading:12176897... | lld:pubmed |
| pubmed-article:12176897 | pubmed:meshHeading | pubmed-meshheading:12176897... | lld:pubmed |
| pubmed-article:12176897 | pubmed:meshHeading | pubmed-meshheading:12176897... | lld:pubmed |
| pubmed-article:12176897 | pubmed:meshHeading | pubmed-meshheading:12176897... | lld:pubmed |
| pubmed-article:12176897 | pubmed:meshHeading | pubmed-meshheading:12176897... | lld:pubmed |
| pubmed-article:12176897 | pubmed:meshHeading | pubmed-meshheading:12176897... | lld:pubmed |
| pubmed-article:12176897 | pubmed:meshHeading | pubmed-meshheading:12176897... | lld:pubmed |
| pubmed-article:12176897 | pubmed:meshHeading | pubmed-meshheading:12176897... | lld:pubmed |
| pubmed-article:12176897 | pubmed:meshHeading | pubmed-meshheading:12176897... | lld:pubmed |
| pubmed-article:12176897 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12176897 | pubmed:articleTitle | B cells immortalized by a mini-Epstein-Barr virus encoding a foreign antigen efficiently reactivate specific cytotoxic T cells. | lld:pubmed |
| pubmed-article:12176897 | pubmed:affiliation | Department of Otorhinolaryngology, Ludwig-Maximilians-Universität, Munich, Germany. moosmann@hno.med.uni-muenchen.de | lld:pubmed |
| pubmed-article:12176897 | pubmed:publicationType | Journal Article | lld:pubmed |
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