| pubmed-article:12164923 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12164923 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:12164923 | lifeskim:mentions | umls-concept:C0022567 | lld:lifeskim |
| pubmed-article:12164923 | lifeskim:mentions | umls-concept:C0021745 | lld:lifeskim |
| pubmed-article:12164923 | lifeskim:mentions | umls-concept:C1948023 | lld:lifeskim |
| pubmed-article:12164923 | lifeskim:mentions | umls-concept:C1150150 | lld:lifeskim |
| pubmed-article:12164923 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:12164923 | pubmed:dateCreated | 2002-8-7 | lld:pubmed |
| pubmed-article:12164923 | pubmed:abstractText | Keratinocytes are an integral component of the skin immune system and function as nonprofessional antigen-presenting cells in pathophysiologic conditions when they express major histocompatibility complex class II molecules, e.g., in psoriasis. In order to analyze further this function we investigated the activity of cathepsin S in comparison with cathepsins B and L. These enzymes were suggested to be involved in antigen presentation. Specific catalytic activities of these cathepsins were determined fluorometrically by hydrolysis of a synthetic substrate (Z-Phe-Arg-7-amido-4-methylcoumarin) in subcellular fractions of human keratinocytes. It was found that the human keratinocyte cell line HaCaT exhibits activities of all three cathepsins investigated. Endosomal/lysosomal compartments show highest cathepsin activities. Normal human keratinocytes in primary culture show a comparable pattern of cathepsin activities. In contrast to this, in syngeneic Epstein-Barr virus-transformed B cells the level of cathepsin B activity was found to be 10% of that in the corresponding keratinocytes, whereas the activities for cathepsins L and S were in a similar range. Interferon-gamma stimulation of primary keratinocytes and HaCaT cells resulted in a selective upregulation of the cathepsin S activity, the extent of which was very similar. The mechanism of this upregulation was demonstrated as induction at the mRNA and protein levels. This report documents that cathepsin S in human keratinocytes is selectively upregulated, in parallel to major histocompatibility complex class II molecules, in response to a pro-inflammatory cytokine. Our observations support the concept of keratinocytes functioning as nonprofessional antigen-presenting cells in states of inflammation. | lld:pubmed |
| pubmed-article:12164923 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12164923 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12164923 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12164923 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:12164923 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12164923 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12164923 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12164923 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:12164923 | pubmed:issn | 0022-202X | lld:pubmed |
| pubmed-article:12164923 | pubmed:author | pubmed-author:WeberEkkehard... | lld:pubmed |
| pubmed-article:12164923 | pubmed:author | pubmed-author:SchröterChris... | lld:pubmed |
| pubmed-article:12164923 | pubmed:author | pubmed-author:KalbacherHube... | lld:pubmed |
| pubmed-article:12164923 | pubmed:author | pubmed-author:BoehnckeWolf-... | lld:pubmed |
| pubmed-article:12164923 | pubmed:author | pubmed-author:SchwarzGerold... | lld:pubmed |
| pubmed-article:12164923 | pubmed:author | pubmed-author:FladThomasT | lld:pubmed |
| pubmed-article:12164923 | pubmed:author | pubmed-author:BraunManuelaM | lld:pubmed |
| pubmed-article:12164923 | pubmed:author | pubmed-author:BursterTimoT | lld:pubmed |
| pubmed-article:12164923 | pubmed:author | pubmed-author:DresselDaniel... | lld:pubmed |
| pubmed-article:12164923 | pubmed:author | pubmed-author:SchmidHeideH | lld:pubmed |
| pubmed-article:12164923 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12164923 | pubmed:volume | 119 | lld:pubmed |
| pubmed-article:12164923 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12164923 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12164923 | pubmed:pagination | 44-9 | lld:pubmed |
| pubmed-article:12164923 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:12164923 | pubmed:meshHeading | pubmed-meshheading:12164923... | lld:pubmed |
| pubmed-article:12164923 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12164923 | pubmed:articleTitle | Cathepsin S activity is detectable in human keratinocytes and is selectively upregulated upon stimulation with interferon-gamma. | lld:pubmed |
| pubmed-article:12164923 | pubmed:affiliation | Medical and Natural Sciences Research Center, University of Tübingen, Ob dem Himmelreich 7, D-72074 Tübingen, Germany. | lld:pubmed |
| pubmed-article:12164923 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12164923 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:1520 | entrezgene:pubmed | pubmed-article:12164923 | lld:entrezgene |
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