| pubmed-article:12145302 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12145302 | lifeskim:mentions | umls-concept:C0134835 | lld:lifeskim |
| pubmed-article:12145302 | lifeskim:mentions | umls-concept:C1145667 | lld:lifeskim |
| pubmed-article:12145302 | lifeskim:mentions | umls-concept:C1510827 | lld:lifeskim |
| pubmed-article:12145302 | lifeskim:mentions | umls-concept:C0332256 | lld:lifeskim |
| pubmed-article:12145302 | lifeskim:mentions | umls-concept:C0205251 | lld:lifeskim |
| pubmed-article:12145302 | lifeskim:mentions | umls-concept:C0084119 | lld:lifeskim |
| pubmed-article:12145302 | pubmed:issue | 42 | lld:pubmed |
| pubmed-article:12145302 | pubmed:dateCreated | 2002-10-15 | lld:pubmed |
| pubmed-article:12145302 | pubmed:abstractText | P-selectin glycoprotein ligand-1 (PSGL-1), a dimeric mucin on leukocytes, is the best characterized ligand for selectins. P-selectin binds stereospecifically to the extreme N terminus of PSGL-1, which contains three clustered tyrosine sulfates (TyrSO3-) adjacent to a Thr residue with a core 2-based O-glycan expressing sialyl Lewis x (C2-O-sLe(x)). GSP-6, a synthetic glycosulfopeptide modeled after the N terminus of PSGL-1, containing three TyrSO3- residues and a short, monofucosylated C2-O-sLe(x) bound to P-selectin with high affinity (K(d) approximately 650 nm). However, PSGL-1 from human HL-60 cells contains higher levels of O-glycans that are sialylated and polyfucosylated polylactosamines (PFPL). Furthermore, studies with fucosyltransferase-deficient mice suggest that sialylated PFPL structures contribute to binding to P-selectin. To resolve whether sialylated PFPL O-glycans participate in binding of PSGL-1 to human P-selectin, we synthesized glycosulfopeptides, designated GSP-6' and GSP-6", with three TyrSO3- residues and either difucosylated polylactosamine (C2-O-Le(x)-sLe(x)) or trifucosylated polylactosamine (C2-O-Le(x)-Le(x)-sLe(x)). Binding of the GSPs to P-selectin was measured by affinity chromatography, fluorescence solid-phase assays, and equilibrium gel filtration. Unexpectedly, both GSP-6' and GSP-6" bound to P-selectin with low affinity (K(d) approximately 37 microm for GSP-6' and K(d) approximately 50 microm for GSP-6"). Binding of GSP-6' and GSP-6" to P-selectin required fucosylation and, to a lesser extent, sialylation as well as the sulfated peptide backbone of GSP-6' and GSP-6". These results demonstrate that contrary to expectations, a core 2 O-glycan containing sialylated PFPL does not promote high affinity binding of PSGL-1 to P-selectin. | lld:pubmed |
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| pubmed-article:12145302 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12145302 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12145302 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12145302 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:12145302 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12145302 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12145302 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:12145302 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:12145302 | pubmed:author | pubmed-author:CummingsRicha... | lld:pubmed |
| pubmed-article:12145302 | pubmed:author | pubmed-author:RenkonenOssiO | lld:pubmed |
| pubmed-article:12145302 | pubmed:author | pubmed-author:McEverRodger... | lld:pubmed |
| pubmed-article:12145302 | pubmed:author | pubmed-author:LeppanenAnneA | lld:pubmed |
| pubmed-article:12145302 | pubmed:author | pubmed-author:PenttilaLeena... | lld:pubmed |
| pubmed-article:12145302 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12145302 | pubmed:day | 18 | lld:pubmed |
| pubmed-article:12145302 | pubmed:volume | 277 | lld:pubmed |
| pubmed-article:12145302 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12145302 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12145302 | pubmed:pagination | 39749-59 | lld:pubmed |
| pubmed-article:12145302 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:12145302 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12145302 | pubmed:articleTitle | Glycosulfopeptides with O-glycans containing sialylated and polyfucosylated polylactosamine bind with low affinity to P-selectin. | lld:pubmed |
| pubmed-article:12145302 | pubmed:affiliation | Department of Biochemistry and Molecular Biology, Oklahoma Center for Medical Glycobiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA. | lld:pubmed |
| pubmed-article:12145302 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12145302 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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