| pubmed-article:12135922 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12135922 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:12135922 | lifeskim:mentions | umls-concept:C0007578 | lld:lifeskim |
| pubmed-article:12135922 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:12135922 | lifeskim:mentions | umls-concept:C0679058 | lld:lifeskim |
| pubmed-article:12135922 | lifeskim:mentions | umls-concept:C1527148 | lld:lifeskim |
| pubmed-article:12135922 | lifeskim:mentions | umls-concept:C0012120 | lld:lifeskim |
| pubmed-article:12135922 | lifeskim:mentions | umls-concept:C0007616 | lld:lifeskim |
| pubmed-article:12135922 | lifeskim:mentions | umls-concept:C0181586 | lld:lifeskim |
| pubmed-article:12135922 | lifeskim:mentions | umls-concept:C1547699 | lld:lifeskim |
| pubmed-article:12135922 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:12135922 | lifeskim:mentions | umls-concept:C0332453 | lld:lifeskim |
| pubmed-article:12135922 | pubmed:issue | 16 | lld:pubmed |
| pubmed-article:12135922 | pubmed:dateCreated | 2002-7-23 | lld:pubmed |
| pubmed-article:12135922 | pubmed:abstractText | The cadA gene in Dictyostelium encodes the Ca2+-dependent cell adhesion molecule DdCAD-1, which is expressed soon after the initiation of development. To investigate the biological role of DdCAD-1, the cadA gene was disrupted by homologous recombination. The cadA-null cells showed a 50% reduction in EDTA-sensitive cell adhesion. The remaining EDTA-sensitive adhesion sites were resistant to dissociation by anti-DdCAD-1 antibody, suggesting that they were distinct adhesion sites. Cells that lacked DdCAD-1 were able to complete development and form fruiting bodies. However, they displayed abnormal slug morphology and culmination was delayed by approximately 6 hours. The yield of spores was reduced by approximately 50%. The proportion of prestalk cells in cadA(-) slugs showed a 2.5-fold increase over the parental strain. When cadA(-) cells were transfected with pcotB::GFP to label prespore cells, aberrant cell-sorting patterns in slugs became apparent. When mutant prestalk cells were mixed with wild-type prespore cells, mutant prestalk cells were unable to return to the anterior position of chimeric slugs, suggesting defects in the sorting mechanism. The wild-type phenotype was restored when cadA(-) cells were transfected with a cadA-expression vector. These results indicate that, in addition to cell-cell adhesion, DdCAD-1 plays a role in cell type proportioning and pattern formation. | lld:pubmed |
| pubmed-article:12135922 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12135922 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12135922 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:12135922 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12135922 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:12135922 | pubmed:issn | 0950-1991 | lld:pubmed |
| pubmed-article:12135922 | pubmed:author | pubmed-author:WangJunJ | lld:pubmed |
| pubmed-article:12135922 | pubmed:author | pubmed-author:FullerDannyD | lld:pubmed |
| pubmed-article:12135922 | pubmed:author | pubmed-author:LoomisWilliam... | lld:pubmed |
| pubmed-article:12135922 | pubmed:author | pubmed-author:WongEstellaE | lld:pubmed |
| pubmed-article:12135922 | pubmed:author | pubmed-author:YangChunzhong... | lld:pubmed |
| pubmed-article:12135922 | pubmed:author | pubmed-author:SiuChi-HungCH | lld:pubmed |
| pubmed-article:12135922 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12135922 | pubmed:volume | 129 | lld:pubmed |
| pubmed-article:12135922 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12135922 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12135922 | pubmed:pagination | 3839-50 | lld:pubmed |
| pubmed-article:12135922 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:12135922 | pubmed:meshHeading | pubmed-meshheading:12135922... | lld:pubmed |
| pubmed-article:12135922 | pubmed:meshHeading | pubmed-meshheading:12135922... | lld:pubmed |
| pubmed-article:12135922 | pubmed:meshHeading | pubmed-meshheading:12135922... | lld:pubmed |
| pubmed-article:12135922 | pubmed:meshHeading | pubmed-meshheading:12135922... | lld:pubmed |
| pubmed-article:12135922 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12135922 | pubmed:articleTitle | Disruption of the gene encoding the cell adhesion molecule DdCAD-1 leads to aberrant cell sorting and cell-type proportioning during Dictyostelium development. | lld:pubmed |
| pubmed-article:12135922 | pubmed:affiliation | Banting and Best Department of Medical Research and Department of Biochemistry, University of Toronto, Toronto, Ontario M5G 1L6, Canada. | lld:pubmed |
| pubmed-article:12135922 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12135922 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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