| pubmed-article:12114502 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12114502 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:12114502 | lifeskim:mentions | umls-concept:C0027740 | lld:lifeskim |
| pubmed-article:12114502 | lifeskim:mentions | umls-concept:C0085103 | lld:lifeskim |
| pubmed-article:12114502 | lifeskim:mentions | umls-concept:C1181031 | lld:lifeskim |
| pubmed-article:12114502 | lifeskim:mentions | umls-concept:C0001471 | lld:lifeskim |
| pubmed-article:12114502 | lifeskim:mentions | umls-concept:C0221099 | lld:lifeskim |
| pubmed-article:12114502 | lifeskim:mentions | umls-concept:C0018270 | lld:lifeskim |
| pubmed-article:12114502 | lifeskim:mentions | umls-concept:C1706968 | lld:lifeskim |
| pubmed-article:12114502 | lifeskim:mentions | umls-concept:C0599177 | lld:lifeskim |
| pubmed-article:12114502 | lifeskim:mentions | umls-concept:C0752319 | lld:lifeskim |
| pubmed-article:12114502 | lifeskim:mentions | umls-concept:C0205224 | lld:lifeskim |
| pubmed-article:12114502 | pubmed:issue | 37 | lld:pubmed |
| pubmed-article:12114502 | pubmed:dateCreated | 2002-9-9 | lld:pubmed |
| pubmed-article:12114502 | pubmed:abstractText | We found in the present study that stimulation of the A(2A) adenosine receptor (A(2A)-R) using an A(2A)-selective agonist (CGS21680) rescued the blockage of nerve growth factor (NGF)-induced neurite outgrowth when the NGF-evoked MAPK cascade was suppressed by an MEK inhibitor (PD98059) or by a dominant-negative MAPK mutant (dnMAPK). This action of A(2A)-R (designated as the A(2A)-rescue effect) can be blocked by two inhibitors of protein kinase A (PKA) and was absent in a PKA-deficient PC12 variant. Activation of the cAMP/PKA pathway by forskolin exerted the same effect as that by A(2A)-R stimulation. PKA, thus, appears to mediate the A(2A)-rescue effect. Results from cAMP-response element-binding protein (CREB) phosphorylation at serine 133, trans-reporting assays, and overexpression of two dominant-negative CREB mutants revealed that A(2A)-R stimulation led to activation of CREB in a PKA-dependent manner and subsequently reversed the damage of NGF-evoked neurite outgrowth by PD98059 or dnMAPK. Expression of an active mutant of CREB readily rescued the NGF-induced neurite outgrowth impaired by dnMAPK, further strengthening the importance of CREB in the NGF-mediated neurite outgrowth process. Moreover, simultaneous activation of the A(2A)-R/PKA/CREB-mediated and the phosphatidylinositol 3-kinase pathways caused neurite outgrowth that was not suppressed by a selective inhibitor of TrkA, indicating that transactivation of TrkA was not involved. Collectively, CREB functions in conjunction with the phosphatidylinositol 3-kinase pathway to mediate the neurite outgrowth process in PC12 cells. | lld:pubmed |
| pubmed-article:12114502 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12114502 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12114502 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12114502 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:12114502 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12114502 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:12114502 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:12114502 | pubmed:author | pubmed-author:ChernYijuangY | lld:pubmed |
| pubmed-article:12114502 | pubmed:author | pubmed-author:ShihHsiu-Ming... | lld:pubmed |
| pubmed-article:12114502 | pubmed:author | pubmed-author:ChengHsiao-Ch... | lld:pubmed |
| pubmed-article:12114502 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12114502 | pubmed:day | 13 | lld:pubmed |
| pubmed-article:12114502 | pubmed:volume | 277 | lld:pubmed |
| pubmed-article:12114502 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12114502 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12114502 | pubmed:pagination | 33930-42 | lld:pubmed |
| pubmed-article:12114502 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:12114502 | pubmed:meshHeading | pubmed-meshheading:12114502... | lld:pubmed |
| pubmed-article:12114502 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12114502 | pubmed:articleTitle | Essential role of cAMP-response element-binding protein activation by A2A adenosine receptors in rescuing the nerve growth factor-induced neurite outgrowth impaired by blockage of the MAPK cascade. | lld:pubmed |
| pubmed-article:12114502 | pubmed:affiliation | Division of Neuroscience, Institute of Biomedical Sciences, Academia Sinica, Taipei 11529, Taiwan, Republic of China. | lld:pubmed |
| pubmed-article:12114502 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12114502 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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